6. TROUBLESHOOTING
Problem
Transmitted-light interference contrast
No Transmitted Light interference contrast is possible.
Integrated Modulation Contrast
IMC cannot be adjusted.
Fluorescence
The image is completely dark (no fluorescence).
The fluorescence is too weak.
Cause/remedy
The specimen is too thick or too thin.
The mounting medium or specimen are of birefringent
material. Rotate the specimen.
The difference in the refractive indices of the specimen
and the mounting medium is too small.
The cover slip is too thick.
Check the Koehler illumination.
Bring the polarizer and analyzer into cross position until
they reach maximum darkness (without specimen).
Check whether the compatible condenser prism and the
corresponding objective prism are configured.
Check the correct placement of the IC condenser prisms.
Check that the correct objective is positioned (eyepoint C
or D).
Check the position of the aperture diaphragm.
For a fixed Transmitted Light arm:
Check that the IMC modulator and the IMC slit diaphragm
slider are inserted correctly and engaged in the IMC posi-
tion.
For a tiltable Transmitted Light arm:
Check that the IMC module is inserted correctly and en-
gaged in the position compatible to the objective.
Check that the correct condenser (S40/0.45 or S80/0.30) is
positioned and that the condenser height is correct.
Switch off the fluorescence filter.
Open the aperture diaphragm completely.
IMC modulator in IMC position.
Open the shutter.
Check the antigen-antibody combination.
Switch over to the active port (or Infinity Port).
Unsuitable specimen (stored incorrectly, too old,
bleached).
Unspecific filter combination.
Objective with too low of a numerical aperture.
Eyepiece magnification too high.
Room where microscope is located too bright.
Incorrect beamsplit on the trinocular tube.
Secondary light due to reflection at condenser.
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Summary of Contents for DMi8
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