68
7. Start-up
7.6.2 Incident Light - Fluorescence
Suitable aperture and field diaphragm values
have been preset for each objective. The inci-
dent light module has also been centered at the
factory.
However, it may be necessary to readjust the in-
cident light module in some cases after trans-
porting and setting up the stand. Therefore,
check the field diaphragm centering.
The following procedure is provided for the inci-
dent light-brightfield illumination.
• Select an objective with moderate
magnification (10x-20x).
• Activate the incident light axis with the
TL/IL
button (84.1).
• Press the
IL-BF / Fluo
button to activate the
brightfield contrast method (one of the variab-
le function buttons on the stand).
• Place a specimen on the stage.
• Focus the specimen using the SmartMove or
the focus wheels.
• Adjust the light intensity with the
INT
buttons
(84.3).
Adjusting the Field Diaphragm
• Close the field diaphragm with the
FD
button
(84.4) or manually until the edge of the
diaphragm (round or rectangular) appears in
the field of view.
• If the limits of the field diaphragm are not in
the center of the field of view, move the
position of the field diaphragm to the center
with the two centering screws (92.1) on the
right side of the stand.
• Use the function buttons
FD
(84.4) to open the
field diaphragm to the point that they just
disappear from the field of view.
• We recommend the use of a rectangular field
diaphragm when using a digital camera.
Match the size of the diaphragm to the chip
size of the camera.
Fig. 92
Adjusting the field diaphragm (incident light-
fluorescence)
1
Adjusting screws for moving the field diaphragm
1