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Lit M00078 Rev 3; September 2016

ENDURO™

GEL XL

User Manual

HORIZONTAL GEL ELECTROPHORESIS UNIT

E0160

E0160-230V

E0160-230V-UK

E160-CAN

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Summary of Contents for ENDURO GEL XL E0160

Page 1: ...Lit M00078 Rev 3 September 2016 ENDURO GEL XL User Manual HORIZONTAL GEL ELECTROPHORESIS UNIT E0160 E0160 230V E0160 230V UK E160 CAN...

Page 2: ...is manual is designed to assist you in the optimal usage of your Enduro GelXL The manual is available in English French German Italian Portuguese and Spanish on our website at www labnetinternational...

Page 3: ...on of the Agarose Gel and Electrophoresis Buffer RNA 5 C Casting the Gel 6 D Removing the Comb 7 E Loading the Samples onto the Gel 8 F Electrical Connections to the Safety Lid 8 G Sample Electrophore...

Page 4: ...ts benzene xylene toluene chloroform carbon tetrachloride alcohols phenols ketones or esters Do not expose the ABS combs supplied with this unit to formaldehyde for extended periods When casting gels...

Page 5: ...y E0164 2 1 mm x 14 28 tooth Reversible Comb E0165 2 1mm 5 8 tooth Reversible comb E0166 Micro casting set 4 6 x 6cm UV Transmittant Casting Tray 2 1mm 5 8 tooth Reversible combs Casting stand with di...

Page 6: ...as a guideline Table 1 Gel Concentrations and Resolving Ranges Table taken from Sambrook J Fritsch E F Maniatis T 1989 Molecular Cloning A Laboratory Manual 1 6 8 613 2 Weigh an appropriate quantity o...

Page 7: ...The addition of ethidium bromide to both the gel and the running buffer will result in maximum detection levels by providing high levels of sample fluorescence with an evenly low level of background 5...

Page 8: ...e blotting efficiency Therefore if samples are to be analyzed by northern hybridization after electrophoresis run a duplicate lane s for staining or minimize the exposure of RNA samples to ethidium br...

Page 9: ...ge 12 C Casting the Gel 1 Place the gel casting stand on a lab bench CAUTION Cast agarose gels containing formaldehyde in a hood 2 Insert the gel casting tray into the casting Stand If you are using t...

Page 10: ...ove the comb s from formaldehyde gels as soon as gel hardening is complete and rinse them well prior to storage If a gel is not to be used immediately after preparation remove it from the casting fixt...

Page 11: ...Power Supply into a wall outlet Ensure an approved power cord that satisfies your regional voltage standard is used Input voltage is automatically detected by the system A transformer is not necessar...

Page 12: ...place within a fume hood Follow the sample migration into the gel using the loading dye as an indicator See Appendix A for the Sample Loading Buffer recipe Allow the samples to migrate until the frag...

Page 13: ...peration stops with alarm The screen displays LOAD Electrophoresis tank is not connected to the power supply or there is a broken circuit in the electrophoresis cell Check the connections to the power...

Page 14: ...Boric Acid 2 0 mM EDTA pH 8 0 6 72g EDTA or 40ml 0 5M EDTA pH 8 0 H2O to 1 liter RNA electrophoresis Running Buffer MOPS Acetate EDTA MAE Solutions containing MOPS should be wrapped in aluminum foil...

Page 15: ...of electrophoresis without removing the gel from the tray Figure A above clearly delineates the absorption specifications of the UV transmittant plastic gel tray Minimal transmission is seen below V R...

Page 16: ...he presence of a potential hazard which could result in electrical shock CAUTION This symbol refers you to important operating and maintenance servicing instructions within the product Instruction Man...

Page 17: ...trical and electronic equipment WEEE Enduro GelXL is marked with the crossed out wheeled bin and must not be disposed of with domestic waste Consequently the buyer shall follow the instructions for re...

Page 18: ...NOTES...

Page 19: ...31 Mayfield Ave Edison NJ 08837 P 732 417 0700 F 732 417 1750 Toll Free 888 522 6381 labnetinfo corning com www labnetinternational com 9300130000...

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