C-16
SNAP* Feline proBNP
Test Description
The SNAP* Feline proBNP Test is a rapid immunoassay to assess the level of NTproBNP (N-terminal
pro-brain natriuretic peptide) in Feline serum or EDTA plasma samples. NTproBNP is released by
the myocardium in response to stress and stretch. A normal result indicates that moderate to severe
heart disease is unlikely at the time of testing. An abnormal result indicates increased stretch and
stress on the heart muscle. As NTproBNP levels may be increased in other conditions, such as
hyperthyroidism, hypertension or severe azotemia, an abnormal result should be evaluated in the
context of complete evaluation of the patient, including history, physical examination, and complete
laboratory profile.
Note:
If the color intensity of the sample spot is equal to or darker than the color intensity of the
reference spot, the NTproBNP level is abnormal. If the color intensity of the sample spot is lighter
than the color intensity of the reference spot, the NTproBNP level is normal.
Preparing and Running the SNAP Feline proBNP Test
Important
: Allow all SNAP Feline proBNP test components to equilibrate at room temperature for at
least 30 minutes. Do not heat.
1. Enter the patient information on the IDEXX VetLab Station (for more information, see the
“Analyzing Samples” chapter of the
IDEXX VetLab Station Operator’s Guide
).
2. Once the patient’s name appears in the Pending list on the SNAPshot Dx Home screen, tap
the patient’s name and then tap
Select
. The Identify Sample screen appears.
3. Select
fBNP
as the SNAP Test Type.
4. Select the applicable sample type.
5. Tap
Optional Prep Instructions
if you would like to view on-screen sample preparation
instructions.
a. Hold the provided pipette vertically and dispense
3 drops of sample
into a disposable
sample tube.
b. Holding the bottle vertically, add
5 drops of green conjugate
to the sample tube.
c. Cap the sample tube and invert it 4–5 times to mix.
6. Tap
Next
and follow the on-screen instructions to properly prepare the SNAP test:
a. Place the device on a horizontal surface.
b. Add the entire contents of the sample tube to the sample well, being careful not to
splash the contents outside of the sample well. The sample will flow across the result
window, reaching the activation circle in 30–60 seconds. Some sample may remain in
the sample well.
c. When color FIRST appears in the activation circle, press the activator firmly until it is
flush with the device body.
Note
: Some samples may not flow to the activation circle within 60 seconds. In this case,
press the activator after the sample has flowed across the result window.
7. Insert the SNAP device into the SNAPshot Dx Analyzer immediately. The SNAPshot Dx
Analyzer automatically begins to process the patient sample and the Home screen displays.
The patient’s name moves from the Pending list to the In Process list.
When analysis is complete, the patient’s name will move from the In Process list to the Results
list (you may also receive a “New Results” alert message and/or hear a beep on the IDEXX
VetLab Station, depending on the option(s) you selected in the New Results Alert tab on the
IDEXX VetLab Station’s Settings screen). You can then remove the SNAP device from the
analyzer by gently pushing the SNAP device until you hear a click. The device will slide out of
the port.
SNAP Test Procedures
Summary of Contents for SNAPshot Dx
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