Idexx SNAPshot Dx, Operator'S Manual

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10. Insert the SNAP device into the SNAPshot Dx Analyzer immediately. The SNAPshot Dx
Analyzer automatically begins to process the patient sample and the Home screen displays.
The patient’s name moves from the Pending list to the In Process list.
When analysis is complete, the patient’s name will move from the In Process list to the Results
list (you may also receive a “New Results” alert message and/or hear a beep on the IDEXX
VetLab Station, depending on the option(s) you selected in the New Results Alert tab on the
IDEXX VetLab Station’s Settings screen). You can then remove the SNAP device from the
analyzer by gently pushing the SNAP device until you hear a click. The device will slide out of
the port.
Total T
4
Dilution Guidelines
The SNAPshot Dx Analyzer allows you to dilute canine and feline Total T
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samples that initially report
>7.0 μg/dL (90.1 nmol/L). Total T
4
dilutions have not been validated on equine samples.
To Ensure that You Receive the Most Accurate Results
• Only perform a dilution when the undiluted sample result is >7.0 μg/dL (90.1 nmol/L).
• Use the lowest dilution factor possible.
• Use only normal saline as the diluent.
Note: Conjugate volume remains at 300 μL even if you are running a diluted sample.
Preparing a 1:2 Dilution (1 part sample, 1 part saline)
1. Using the purple handheld pipette, dispense 65 μL of sample (serum or plasma) into a
disposable sample tube (e.g., a disposable tube from the SNAP Total T
4
Test Kit).
2. Using the purple handheld pipette, add 65 μL of normal saline to the tube.
3. Cap the tube and gently invert 4–5 times to mix the sample and saline.
4. Using the purple handheld pipette, aspirate 65 μL of the diluted sample and run the SNAP
Total T
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Test as normal.
Note : When prompted, select 1 in the Dilution drop-down list.
Preparing a 1:4 Dilution (1 part sample, 3 parts saline)
Important: A 1:4 dilution is only recommended if the result of a 1:2 diluted sample is
>14.0 μg/dL (180.2 nmol/L).
1. Using the purple handheld pipette, dispense 65 μL of sample (serum or plasma) into a
disposable sample tube (e.g., a disposable tube from the SNAP Total T
4
Test Kit).
2. Using the purple handheld pipette, add 65 μL of normal saline to the tube 3 times.
3. Cap the tube and gently invert 4–5 times to mix the sample and saline.
4. Using the purple handheld pipette, aspirate 65 μL of the sample/saline combination and
dispense it into a new disposable sample tube.
5. Using the diluted sample, prepare and run the SNAP Total T
4
Test as normal.
Note : When prompted, select 3 in the Dilution drop-down list.
SNAP Test Procedures