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SQUIRE
PLUS
2
OPERATIONS
MANUAL
PROCESS & PACKAGING, INC.
P
AGE
44
OF
125
www.iddeas.com
water zones) of the pasteurizer. A 20 to 30 ppm solution is normally adequate and safe on
equipment
Tank-Truck Washing:
As in the typical use for tank washing and sanitizing, stabilized ClO
2
can be used for the sanitization and cleanup
of bulk tankers and related pipes and hoses. Wash down tank internals, pumps, and hoses at 100 ppm,
externals at 250 to 500 ppm. This will prevent transfer of micro-organisms to or from the connecting systems.
Washing Of Yeast Slurry With Stabilized CLO
2
(Oxine):
Stabilized ClO
2
(Oxine) will effectively kill unwanted bacteria in yeast at 20 to 40 ppm, while leaving the culture
yeast unharmed. Because of stabilized ClO
2
's unique action, it does not have the drawbacks of phosphoric acid
because it does not form chlorophenols (which contribute to a much feared medicinal character in beers) in the
presence of organic materials.
Mold And Mildew Cleanup And Control:
Clean off walls and floors as well as air handling units and machinery using a 200 to 500 ppm solution of
stabilized ClO
2
. This effectively kills mold and mildew spores while still retaining some bleaching ability to remove
the tough, black stains. This is good procedure prior to painting, or prior to the application of a mold and slime
growth inhibitor such as
Alltech's Mold-Zap
.
Process Water Treatment:
The FDA has approved stabilized ClO
2
for process water sanitization at between 100 to 200 ppm available ClO
2
.
Potable water treatment is approved for human consumption at 4 ppm available ClO
2
.
Glass Washing/Rinsing:
Even at the final stage of the brewer's trade, where bars and pubs have a need for an effective and safe
drinking-glass sanitizer, stabilized ClO
2
can fill the need. Since stabilized ClO
2
can be safely consumed at 4 ppm,
it makes an ideal glass rinse. Unlike typical quaternary ammonium rinses, it will not have any deleterious effect
on beer flavor, nor on beer foam quality. Use at 20 to 50 ppm in final rinse water.
W
ASHING
M
ETHOD
:
Normally, an initial washing at 20 to 40 ppm (20 ppm for homogenous yeast, 40 ppm for "clumpy" or highly
flocculant strains) is effective against most Lactobacilli and Pediococci species. This "wash" is accomplished by
adding stabilized ClO
2
directly to yeast slurry on a volume/ppm basis. It does not need to be activated as the pH of
the yeast slurry is low enough to convert to ClO
2
.
Stabilized ClO
2
is supplied at a concentration of 20,000 ppm or 2% Chlorine Dioxide when activated. Therefore, on
a volume basis with one gallon of stabilized ClO
2
concentrate, can be made one thousand gallons of solution at 20
ppm. By this method, we can also assume with one gallon of concentrate to make up one thousand gallons of
yeast slurry at 20 ppm. It is impractical to measure the concentration of ClO
2
in the presence of yeast, so the
dilution must be made based on the strength of the concentrate.
Yeast slurry should be thin enough to allow for homogeneous mixing, usually 20 to 40% solids. The killing action of
stabilized ClO
2
is very fast compared to the traditional phosphoric acid wash with only 15 to 30 minutes needed for
the full bactericidal effect of stabilized ClO
2
to occur, with no resultant stress on yeast cells.
D
OSING
R
ATE
–
N
ORMAL
W
ASH
:
For regular washing of homogenous recycled brewer’s yeast, only a 20 to 40 ppm wash for a minimum of 30
minutes to 5 hours prior to pitching is required. Mix 1 to 2 ml of stabilized ClO
2
concentrate with each liter of yeast
slurry and agitate thoroughly during this process.
Summary of Contents for SQUIRE PLUS 2
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