INS0057 EN
DEC 2014
Rev. E
Directions for Use -
Aquasnap™ Total & Aquasnap™ Free
ATP Tests for Water Samples
Part Number: Aquasnap Total - AQ-100X (100 tests)
Aquasnap Free - AQ-100FX (100 tests)
Description / Intended Use:
Aquasnap
TM
water testing devices are self-contained ATP tests
for use with Hygiena luminometers. Tests are used to monitor
ATP levels in water as a quality indicator in areas like Clean-In-
Place (CIP) systems and rinse water samples. It is also used for
water treatment applications in healthcare and monitoring
biomass in cooling towers. Aquasnap
TM
Total measures both
ATP contained within living cells and particulate matter
(microbial ATP) as well as ATP dissolved in water (non-
microbial or dead microbial ATP). Aquasnap
TM
Free measures
only dissolved ATP outside of living cells (non-microbial ATP).
Used together, Aquasnap
TM
Total and Aquasnap
TM
Free can be
an effective quality monitoring system. The difference between
Total and Free test results represents ATP from living
organisms (also referred to as biomass).
Total ATP= Free ATP + Microbial ATP, therefore,
Microbial ATP = Total ATP
– Free ATP
For more information about differences between Aquasnap
TM
Total and Aquasnap
TM
Directions:
Instructional Video:
www.youtube.com/HygienaTV
1.
Allow Aquasnap
TM
test devices to equilibrate to room
temperature (21-25°C) before use. Forcefully flick device in
a downward motion to shake liquid extractant from
collection dipper to bottom of tube. Shaking liquid
extractant to bottom of tube assists with accurate
extraction of ATP and helps collect a more consistent
sample. Holding swab tube firmly, twist and pull collection
device out of tube. Submerge sample collection dipper in
water sample for 1-2 seconds.
2.
Lift sample collection device up vertically and reinsert in
test tube. Gently shake device for 1-2 seconds to release
water sample from collection tip and to mix sample with
extractant at bottom of test tube.
3.
Activate
Aquasnap
TM
by holding tube firmly and using
thumb and forefinger to break
Snap Valve™ by bending
bulb forward and backward. Squeeze bulb twice, expelling
all liquid into tube.
4.
Shake for 3-5 seconds to mix sample.
5.
Insert entire Aquasnap
TM
device into luminometer, close lid
and press “OK” to initiate measurements. Device should
be measured within 15 seconds of activation.
6.
Hygiena recommends setting RLU thresholds according to
user’s test application. Higher RLU results indicate higher
contamination in sample. In clean or treated water
samples, Free ATP results are similar to Total ATP results.
In some circumstances, when organic matter is present
and where microbial contamination is low, Total ATP
results may appear lower than Free ATP results; this is
normal and is due to presence of extractant in Aquasnap
TM
Total. To determine Pass/Fail settings, visit the resource
library at
www.hygiena.com
or email a technical
representative at:
Sampling
Recommendations:
When possible, split water sample into two aliquots. Dip each
Aquasnap
TM
test (Total and Free) in a separate aliquot of same
water sample. If two aliquots of water sample cannot be made
and two devices have to be dipped in same sample container,
use Aquasnap
TM
Free first, then Aquasnap
TM
Total.
Calibration Control:
It is advisable to run positive and negative controls according to
Good Laboratory Practices. Hygiena offers the following control:
Calibration Control Kit (Part # PCD4000)
Safety & Precautions:
Components of Aquasnap
TM
do not pose any health risk when
used correctly.
1.
Do not use Aquasnap
TM
test if it is already activated.
2.
Hold luminometer upright when measuring test devices.
3.
Hold Aquasnap
TM
test upright when activating.
4.
Read Aquasnap
TM
test within 15 seconds of activation.
5.
Keep Aquasnap
TM
test out of direct sunlight.
Storage & Shelf Life:
Store at 2-8°C. Devices may be stored at room temperature
(21-25°C) for up to 4 weeks. Aquasnap
TM
has a shelf life of 15
months. Check expiration date on label.
Hygiena Liability:
Hygiena will not be liable to user or others for any loss or damage
whether direct or indirect, incidental or consequential from use of
this device. If this product is prov
en to be defective, Hygiena’s
sole obligation will be to replace product or at its discretion, refund
the purchase price. Promptly notify Hygiena within 5 days of
discovery of any suspected defect and return product to Hygiena.
Contact information:
If more information is required, please visit us at
www.hygiena.com
or contact us at:
Hygiena - Americas
Phone: 805.388.8007
Fax: 805.388.5531
Email:
Hygiena - International
Phone: +44 (0)1923 818821
Fax: +44 (0)1923 818825
Email:
