HemoCue WBC DIFF, Operating Manual, Page: 41 / 84

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Important! Always handle blood specimens with care, they might be infectious.
Consult local environmental authorities for proper disposal. Always wear protective
gloves when handling blood specimens. The microcuvette is for single use only.
For the measuring procedure see Routine Use/QC Test or Routine Use/Patient Test .
Control material shall always be measured using QC test procedure.
1. Venous blood samples shall be stored at room temperature (18–30 °C, 64–86°F) and
analyzed within eight hours after sample collection. Mix the venous sample tubes
thoroughly using a roller mixer for 1-2 minutes or invert the tube 10–20 times by hand.
Samples may not be diluted. For control material always follow instructions for use
provided by the manufacturer.
2. Place a drop of blood or control material onto a hydrophobic surface using a pipette (2a)
or other suitable transfer device (2b).
3. Fill the microcuvette in one continuous process. Do NOT refill!
NOTE: Make sure that the microcuvette is filled from the tip, forming an angle (about
45 °) with the blood drop.
4. Wipe off excess blood from the outside of the microcuvette with a clean, lint-free wipe.
Do not touch the open end of the microcuvette.
5. Look for air bubbles in the filled microcuvette. If present, discard the microcuvette and
fill a new microcuvette from a new drop of blood.