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Hamamatsu Photonics C13440-20CU Instruction Manual Download Page 83

C13440-20CU / C13440-20CU01 Instruction manual_Ver.1.3 

 

 

83 

 

 

Camera Link interface 

USB 3.0 interface 

Readout mode 

Binning readout mode 2×2,4×4 (Digital binning) *

3

 

Sub-array readout mode 

(Configurable for each vertical 4 

pixels and horizontal 128 pixels.) 

Sub-array readout mode 

(Configurable for each vertical 4 

pixels and horizontal 128 pixels.) 

Lightsheet 
Readout Mode 

Readout time 

20 ms to 204.8 s 

Readout mode 

Full/Sub-array 

(Configurable for each vertical 4 pixels and horizontal 128 pixels.) 

Readout direction 

Top to bottom readout / Bottom to top readout 

W-VIEW mode 

Readout mode 

Binning readout mode 2×2,4×4 (Digital binning) *

3

 

Sub-array readout mode 

(Configurable for each vertical 4 pixels and horizontal 128 pixels, the 

position can be set independently for top and bottom area.) 

Readout direction 

Top to bottom readout / Bottom to top readout 

(The direction can be set independently for top and bottom area.) 

Dual Lightsheet 
Readout Mode 

Readout time 

10 ms to 102.4 s 

Readout mode 

Full/Sub-array 

(Configurable for each vertical 4 pixels and horizontal 128 pixels, and 

the position can be set independently for top and bottom area.) 

Readout direction 

Top to bottom readout / Bottom to top readout 

(The direction can be set independently for top and bottom area.) 

Exposure time

 

Free running mode 

Standard scan 

1 ms to 10 s 

Slow scan 

3 ms to 10 s 

Free running mode 
/ Sub-array mode 

Standard scan 

38.96 µs to 10 s 

Slow scan 

129.99 µs to 10 s 

External trigger 
mode 

Standard scan 

1 ms to 10 s 

Slow scan 

3 ms to 10 s 

Free running mode 
/ W-VIEW mode *

5

 

Standard scan 

1 ms to 2 s 

Slow scan 

3 ms to 6 s 

Free running mode 
/ Sub-array mode 
/ W-VIEW mode *

5

 

Standard scan 

38.96 µs to 2 s   

(The same exposure time is set for top and bottom areas when the 

exposure time is 1ms or shorter.) 

Slow scan 

129.99 µs to 6 s 

(The same exposure time is set for top and bottom areas when the 

exposure time is 3ms or shorter.) 

External trigger 
mode / W-VIEW 
mode *

5

 

Standard scan 

1 ms to 2 s 

Slow scan 

3 ms to 6 s 

Lightsheet Readout Mode 

9.7 µs to 10 s 

(The exposure time is the same for top and bottom area.) 

Dual Lightsheet Readout Mode 

External trigger 
Function 

Normal area mode 

Edge trigger mode / Global reset Edge trigger mode / 

Level trigger mode / Global reset Level trigger mode / 

Synchronous readout trigger mode / 

Start trigger mode 

Lightsheet Readout 
Mode 

Edge trigger mode / Start trigger mode 

W-VIEW mode   

Edge trigger mode / Global reset Edge trigger mode 

(The exposure time can be set independently for top and bottom area.) 

Level trigger mode / Global reset Level trigger mode 

(The exposure time is the same for top and bottom area.) 

Synchronous readout trigger mode 

(The exposure time is the same for top and bottom area.) 

Start trigger mode 

(The exposure time can be set independently for top and bottom area.) 

Dual Lightsheet 
Readout Mode 

Edge trigger mode / Start trigger mode 

Summary of Contents for C13440-20CU

Page 1: ...oid personal injury and damage to property when using this system The manual describes the correct handling method of the system and provides instructions that should be followed to avoid accidents Re...

Page 2: ...Blank page...

Page 3: ...d even death Improper handling of the camera without observing these cautions could lead to personal injury to the user or damage to property This symbol indicates a cautionary item that should be fol...

Page 4: ...can lead to electric shock Do not attempt to dismantle or modify the camera Doing so can also lead to damage and even injury as some internal components become very hot or high voltage Do not touch p...

Page 5: ...into the C mount of the camera Doing so can scratch the protective glass Some wide angle lenses in particular can have a thread of 7 mm or more Shipping precautions When transporting the camera by tru...

Page 6: ...is attached to your circulating water cooler for an appropriate temperature range of cooling water If you plan on using water other than soft water as recommended for example antifreeze etc please ref...

Page 7: ...D ROM 1 QC sheet 1 Option Cooling water hose 2 hoses A10788 04 SMA BNC cable A12106 05 SMA SMA cable A12107 05 Camera Link interface board M9982 29 Camera Link interface cable A14038 04 USB 3 0 interf...

Page 8: ...of magnetism or ratio waves Places where there are vibrations Places where the camera may come into contact with corrosive gases such as chlorine or fluorine Places where there is a lot of dust How t...

Page 9: ...IMAGING 25 9 4 END OF IMAGING 25 9 5 STARTUP DCAM CONFIGURATOR 26 10 DESCRIPTION OF CMOS IMAGE SENSOR 28 10 1 THEORY OF CMOS IMAGE SENSOR 28 10 2 READOUT METHOD OF CMOS IMAGE SENSOR 29 10 3 PRECAUTION...

Page 10: ...CATIONS 82 14 2 CONDENSATION 85 14 3 SPECTRAL RESPONSE CHARACTERISTICS TYP 86 14 4 INTERFACE SPECIFICATIONS 87 14 5 OUTPUT TIMING SPECIFICATIONS 89 15 DIMENSIONAL OUTLINES 93 15 1 C13440 20CU 93 15 2...

Page 11: ...6 electrons r m s readout noise performance Moreover the camera delivers high sensitivity through its on chip micro lens 37 000 1 high dynamic range that makes the camera suitable for almost any scien...

Page 12: ...mage sensor has 6 5 m x 6 5 m pixel sizes that is equivalent to conventional CCD image sensor 2 3 inch 1 3 megapixels Also the camera can observe a wider field of view because the pixel number is abou...

Page 13: ...data transfer is not required This interface does not require a Camera Link interface board It transfers image with moderate transfer speed When a connection interface is changed from Camera Link to U...

Page 14: ...operation modes External trigger modes Normal Area Mode Free running mode External trigger mode Edge trigger mode Start trigger mode Global reset Edge trigger mode Level trigger mode Global reset Lev...

Page 15: ...0 20CU C13440 20CU01 Instruction manual_Ver 1 3 15 6 NAME AND FUNCTION OF PARTS 1 C13440 20CU C mount type Figure 6 1 2 C13440 20CU01 F mount type Figure 6 2 b a d c e k f g h i j l b a d c e k f g h...

Page 16: ...2 on the computer When a connection interface is changed from Camera Link to USB 3 0 and vice versa the application software must be closed and the camera must be turned off Do not connect Camera Lin...

Page 17: ...nterface is changed from Camera Link to USB 3 0 and vice versa the application software must be closed and the camera must be turned off Do not connect Camera Link and USB 3 0 interface simultaneously...

Page 18: ...not place the rear panel of the camera which connectors are located to be at the bottom Do not block ventilation openings When you connect cables turn off the power supply of the camera and the perip...

Page 19: ...be closed and the camera must be turned off Do not connect Camera Link and USB 3 0 interface simultaneously Note Hamamatsu recommends A14038 04 optional Camera Link interface cable for this camera Th...

Page 20: ...mmended to use soft water except pure water for cooling water If you plan on using water other than soft water as recommended for example antifreeze etc please refer to description of cooling water wh...

Page 21: ...Figure 8 1 If the hose size on circulating water cooler is the same as blue hose remove gray hose from the joint part The gray hose can be removed when blue hose is pulled with pushing the button of t...

Page 22: ...d on the microscope If it is easy to connect the hose onto the camera after the camera is set onto the microscope then it is OK to connect the hose after the camera is set on the microscope 4 Connect...

Page 23: ...e water or water drop inside the hose and the camera by air Blow air from one side of hose Prepare water absorption sheet such as Waste Towel or so and catch pan on another side of hose in order to av...

Page 24: ...culating water cooler Optional is used for cooling the heated side of a peltier element Cooling does not start just turning on the camera Cooling water circulation must be started before start operati...

Page 25: ...ing properly and air is circulating When cooling method of the camera is set by water cooling method the fan does not start rotating 9 2 2 WHEN USING WATER COOLING 1 Connect the equipment as shown in...

Page 26: ...startup DCAM Configurator 1 Open Setup exe in the DCAM API software s folder If the DCAM API software is not installed on your computer insert the media of DCAM API software in the slot of your comput...

Page 27: ...f the camera s power supply is turned off the state of setting is kept The state of setting can confirm according to Hardware icon on DCAM Configurator window After the startup operate DCAM Configurat...

Page 28: ...switch one by one Figure 10 1 The scientific CMOS image sensor used in this camera has an on chip CDS correlated double sampling circuit which plays an important role in achieving low noise In additi...

Page 29: ...s may cause failure in part of the silicon wafer resulting in white spots Currently this phenomenon is not preventable If CMOS image sensor is at a fixed temperature recurrence of the white spot incre...

Page 30: ...ent pixels in the digital domain Binning readout is a method for achieving high sensitivity in exchange for losing resolution 3 Sub array readout Sub array readout is a procedure only a region of inte...

Page 31: ...ink interface is necessary to transfer the image data with 100 fps of fast frame rate for full resolution When you use USB 3 0 interface the frame rate is up to 40 fps 16 bit of digital output for ful...

Page 32: ...801 128 1603 64 3206 8 25 655 External trigger mode Edge trigger Level trigger 1 Vn 2 1H Exp1 10 1H 2048 2048 90 1024 164 512 278 256 425 128 579 64 707 8 877 External trigger mode Synchronous readout...

Page 33: ...12 512 400 512 8 25 655 2 2 64 Hn 1024 4 Vn 1024 1 Vn 1H 1024 1024 100 4 4 32 Hn 512 2 Vn 512 1 Vn 2 1H 512 512 100 External trigger mode Edge trigger Level trigger 1 1 Hn 512 V 152 1 round Vn 2048 40...

Page 34: ...ger mode Edge trigger Level trigger 1 1 Hn 512 Vn 256 1 round Vn 2048 53 1H 1H 2048 2048 53 2048 512 212 Hn 512 Vn 248 1 Vn 2 1H Exp1 10 1H 2048 64 707 2048 8 877 Hn 512 8 Vn 2048 1 Vn 2 1H Exp1 10 1H...

Page 35: ...Hn 512 Vn 904 1 Vn 2 1H Exp1 10 1H 2048 512 278 2048 64 707 2048 8 877 Hn 512 8 Vn 2048 1 Vn 2 1H Exp1 10 1H 512 2048 90 512 512 278 512 8 877 2 2 64 Hn 1024 4 Vn 1024 1 Vn 1H Exp1 10 1H 1024 1024 90...

Page 36: ...120 256 240 128 481 64 962 8 7696 External trigger mode Edge trigger Level trigger 1 Vn 2 1H Exp1 10 1H 2048 2048 27 1024 50 512 85 256 133 128 184 64 228 8 287 External trigger mode Synchronous reado...

Page 37: ...to 10 s 129 99 s to 10 s with sub array setting input in units of seconds Exp2 Exp1 32 4812 s round up to integer Calculation formula 32 4812 s Exp2 Available setting range of the exposure time is th...

Page 38: ...idiary or your local distributor for the detail of the timing information 1 Normal readout The normal readout mode is suitable for observation monitoring field of view and focus adjustment and animati...

Page 39: ...r signal level when signal overflow happens due to too much input photons in normal readout mode In this mode the fastest frame rate is 100 fps standard scan via Camera Link 40 fps standard scan via U...

Page 40: ...ternal signal Exposure time is set In this mode the exposure of the first line begins on the edge rising falling timing of the input trigger signal into the camera 1023H and 1024H in the following fig...

Page 41: ...eset Edge trigger mode the exposure of all pixels begins on the edge rising falling timing of the input trigger signal into the camera Figure 11 6 Ex rising edge 0H 1H 1023H 1024H 2046H 2047H Sensor r...

Page 42: ...nd the exposure of the second line begins after the readout time of line one passes Each exposure begins one by one for each line The exposure of the first line is finished when the trigger signal bec...

Page 43: ...for the trigger level High With this Global reset Level trigger mode the exposure of all pixels begins when the trigger signal becomes High Figure 11 8 Ex level High 0H 1H 1023H 1024H 2046H 2047H Sens...

Page 44: ...r signals Normal operation when the Trigger Time is set as 1 The Synchronous readout trigger mode is used for continuous imaging when it is necessary to control the exposure start timing of each frame...

Page 45: ...exposure time The following figure shows the exposure timing when the Trigger Times is set as 3 Figure 11 10 Trigger Times Sensor readout Camera Link data output USB 3 0 data output Global exposure ti...

Page 46: ...rnal trigger mode by the edge of an external trigger signal rising falling edge Figure 11 11 Ex rising edge 11 1 6 4 External trigger delay function In most cases when a delay between the laser pulse...

Page 47: ...exposure timing output shows the period where all lines expose at the same time Note There is no output signal when the exposure time is less than the frame rate 2 Programmable timing output By using...

Page 48: ...s working in the Edge trigger mode the next frame can start after the previous frame exposure is done Thus the camera cannot accept a trigger for the next frame during the exposure period To reduce us...

Page 49: ...www hamamatsu com jp en technology innovation lightsheetreadout index html 11 2 1 READOUT DIRECTION The camera reads out from the center line to the top line and to the bottom line simultaneously in...

Page 50: ...a is used with DCAM 2 Camera operation modes This mode can use Free running mode Edge trigger mode External trigger mode and Start trigger mode 11 2 3 FRAME RATE CALCULATION The frame rate calculation...

Page 51: ...512 2048 49 512 512 196 2 12 bit 8 bit Digital output Readout Horizontal Calculation formula Hn Vn Frame rate fps Vertical 1 1 128 Hn 2048 4 Vn 2048 1 Exp1 Vn 10 1H 2048 2048 49 2048 512 196 2048 64...

Page 52: ...3440 20CU01 Instruction manual_Ver 1 3 52 11 2 5 TIMING DIAGRAM 1 Free running mode Figure 11 17 Ex Top to bottom readout 2 Edge trigger mode External trigger mode Figure 11 18 Ex rising edge Top to b...

Page 53: ...C13440 20CU C13440 20CU01 Instruction manual_Ver 1 3 53 3 Start trigger mode Figure 11 19 Ex rising edge Top to bottom readout...

Page 54: ...h external devices is simple A system which needs simple timing signal does not require a delay unit or pulse generator It is possible to program and output a pulse that has an optional pulse width an...

Page 55: ...1 21 Programmable timing output referenced with Hsync Top to bottom readout When you choose Hsync for the reference of programmable timing output camera can output some pulses before start the exposur...

Page 56: ...ed for simultaneous image acquisition of dual wavelength images 11 3 1 READOUT DIRECTION The readout direction can be set for the top half area and the bottom half area independently in W VIEW Mode Fi...

Page 57: ...Size Position Horizontal Vertical Horizontal Vertical 128 pixels 4 lines 4 pixels 4 lines Note Minimum settable step of the size and position on the table is in only the case that the camera is used w...

Page 58: ...100 512 200 256 400 128 801 64 1603 32 3206 4 25 655 External trigger mode Edge trigger Level trigger 1 Vn 1H Exp1 10 1H 2048 1024 90 512 164 256 278 128 425 64 579 32 707 4 877 External trigger mode...

Page 59: ...655 2 2 64 Hn 1024 2 Vn 512 1 Vn 2 1H 1024 512 100 4 4 32 Hn 512 1 Vn 256 1 Vn 4 1H 512 256 100 External trigger mode Edge trigger Level trigger 1 1 Hn 512 Vn 76 1 round Vn 1024 40 1H 1H 2048 1024 40...

Page 60: ...6 100 External trigger mode Edge trigger Level trigger 1 1 Hn 512 Vn 128 1 round Vn 1024 53 1H 1H 2048 1024 53 2048 256 212 Hn 512 Vn 124 1 Vn 1H Exp1 10 1H 2048 32 707 2048 4 877 Hn 512 4 Vn 1024 512...

Page 61: ...Vn 456 1 round Vn 1024 80 1H 1H 2048 1024 80 Hn 512 Vn 452 1 Vn 1H Exp1 10 1H 2048 256 278 2048 32 707 2048 4 877 Hn 512 4 Vn 1024 512 1024 90 512 256 278 512 4 877 2 2 64 Hn 1024 2 Vn 512 1 Vn 2 1H E...

Page 62: ...8 1024 30 512 60 256 120 128 240 64 481 32 962 4 7696 External trigger mode Edge trigger Level trigger 1 Vn 1H Exp1 10 1H 2048 1024 27 512 50 256 85 128 133 64 184 32 228 4 287 External trigger mode S...

Page 63: ...to the calculation formula Exp2 Exp1 32 4812 s round up to integer input in units of seconds to the calculation formula Calculation formula 32 4812 s Exp2 Available setting range of the exposure time...

Page 64: ...unning mode Electrical shutter mode works for the half area whose exposure time is shorter than the other half area exposure in W VIEW Mode When the exposure time for the both half areas are shorter t...

Page 65: ...posure time for both half areas is the same with Level trigger mode Global reset Level trigger mode and Synchronous readout trigger mode Note The each timing diagram of Level trigger mode Global reset...

Page 66: ...28 Global reset Edge trigger mode Ex Top top to center Bottom center to bottom Figure 11 29 Start trigger mode Ex Top top to center Bottom center to bottom Note Please contact a Hamamatsu subsidiary o...

Page 67: ...mable timing output synchronizing external devices is simple A system that needs simple timing signal does not require a delay unit or pulse generator It is possible to program and output a pulse that...

Page 68: ...t Mode can achieve 2 time faster frame rate than that of Lightsheet Readout Mode The position of sub array readout can be set for the top half area and the bottom half area independently in Dual Light...

Page 69: ...he camera is used with DCAM 2 Camera operation modes This mode can use Free running mode Edge trigger mode External trigger mode and Start trigger mode 11 4 3 FRAME RATE CALCULATION The frame rate cal...

Page 70: ...2 1282 Hn 512 Vn 4 1 Exp1 Vn 10 1H 2048 4 6841 Hn 512 4 Vn 1024 1 Exp1 Vn 10 1H 512 1024 99 512 256 384 2 12 bit Digital output Readout Horizontal Calculation formula Hn Vn Frame rate fps Vertical 1 1...

Page 71: ...Vn 1H The range of exposure time with Lightsheet Readout Mode is as shown below The maximum exposure time can be decided according to the readout time of a frame which is longer than 10 s or not 1H t...

Page 72: ...20CU01 Instruction manual_Ver 1 3 72 2 Edge trigger mode Figure 11 36 Ex rising edge Top top to center Bottom center to bottom 3 Start trigger mode Figure 11 37 Ex rising edge Top top to center Botto...

Page 73: ...does not require a delay unit or pulse generator It is possible to program and output a pulse that has an optional pulse width and an optional delay time to Read End the end of readout timing Vsync or...

Page 74: ...the silicon wafer in CMOS image sensor The camera has real time variant pixel correction features to improve image quality The correction is performed in real time without sacrificing the readout spe...

Page 75: ...mount of data by compressing data of unrequired area User can specify the required area in units of 4 x4 pixels using camera driver software DCAM API The DCAM API reconstructs the original image from...

Page 76: ...t and 8 bit digital output The steps of gradation for each pixel would be reduced with 12 bit and 8 bit digital output However the look up table function can minimize the lack of gradation by choosing...

Page 77: ...r This function can be turned ON and OFF Default is OFF The master pulse supports free running mode start trigger mode and burst mode The range of interval time is 10 s to 10 s and the step is 1 s for...

Page 78: ...stops generating pulses after the specified number of pulses are generated And then the camera will be ready for the next input trigger signal Figure 11 45 Camera Normal area Edge trigger mode The nu...

Page 79: ...center area of the window gently 3 Confirm whether dust is not left Attach the camera to an optics and check if there is dust or not under the uniform light condition If there is dust on the image pl...

Page 80: ...ssible to use distilled water or deionized water by conducting periodical check However please notice it increases possibility of corrosion inside cooling water path lowering flow rate or water flow s...

Page 81: ...asures Chapter Scratches or discoloration visible on the screen Lens is dirty Wipe the lens 12 Image is blurred Lens is not focused Contact a Hamamatsu subsidiary or your local distributor 17 Condensa...

Page 82: ...8 electrons median 1 4 electrons r m s Dark current 1 at 10 C 0 06 electrons pixel s at 30 C 0 006 electrons pixel s Dynamic range 2 37 000 1 Dark Signal Non Uniformity DSNU 1 0 3 electrons r m s Phot...

Page 83: ...s Slow scan 3 ms to 10 s Free running mode Sub array mode Standard scan 38 96 s to 10 s Slow scan 129 99 s to 10 s External trigger mode Standard scan 1 ms to 10 s Slow scan 3 ms to 10 s Free running...

Page 84: ...f Low Medium High Data extraction Interface Camera Link Full configuration 4 USB 3 0 Super Speed Lens mount C mount C13440 20CU F mount C13440 20CU01 1 Typical value 2 Calculated from the ratio of the...

Page 85: ...foot when making it move because it is approx 2 2 kg Note Please refer to 15 DIMENSIONAL OUTLINES for detail of dimensions 5 Applicable standards EMC EN61326 1 2013 Class A 14 2 CONDENSATION At the Wa...

Page 86: ...C13440 20CU C13440 20CU01 Instruction manual_Ver 1 3 86 14 3 SPECTRAL RESPONSE CHARACTERISTICS TYP Figure 14 2...

Page 87: ...16 11 X1 4 23 X2 17 10 X2 5 22 Xclk 18 9 Xclk 6 21 X3 19 8 X3 7 20 SerTC 20 7 SerTC 8 19 SerTFG 21 6 SerTFG 9 18 CC1 22 5 CC1 10 17 CC2 23 4 CC2 11 16 CC3 24 3 CC3 12 15 CC4 25 2 CC4 13 13 Inner Shie...

Page 88: ...D4_5 TxIN17 Port C1 D1_1 Port F3 D2_11 Port I6 D4_6 TxIN18 Port C2 D1_2 Port F4 D2_12 Port I7 D4_7 TxIN19 Port C3 D1_3 Port F5 D2_13 Port J0 D4_8 TxIN20 Port C4 D1_4 Port F6 D2_14 Port J1 D4_9 TxIN21...

Page 89: ...L H20 L H0 U H5 U H10 U H15 U H20 U H2035 U H2040 U H2045 U H0 U H5 U H10 U H15 U H20 U L Lower byte U Upper byte H1 L H6 L H11 L H16 L H21 L H2036 L H2041 L H2046 L H1 L H6 L H11 L H16 L H21 L H1 U H...

Page 90: ...H5 U H10 U H15 U H20 U L Lower byte U Upper byte H1 L H6 L H11 L H16 L H21 L H1011 L H1016 L H1021 L H1 L H6 L H11 L H16 L H21 L H1 U H6 U H11 U H16 U H21 U H1011 U H1016 U H1021 U H1 U H6 U H11 U H16...

Page 91: ...U H500 U H505 U H510 U H0 U H5 U H10 U H15 U H20 U L Lower byte U Upper byte H1 L H6 L H11 L H16 L H21 L H501 L H506 L H511 L H1 L H6 L H11 L H16 L H21 L H1 U H6 U H11 U H16 U H21 U H501 U H506 U H51...

Page 92: ...1 L H16 L H21 L H2036 L H2041 L H2046 L H1 L H6 L H11 L H16 L H21 L H1 U H6 U H11 U H16 U H21 U H2036 U H2041 U H2046 U H1 U H6 U H11 U H16 U H21 U H2 L H7 L H12 L H17 L H22 L H2037 L H2042 L H2047 L...

Page 93: ...C13440 20CU C13440 20CU01 Instruction manual_Ver 1 3 93 15 DIMENSIONAL OUTLINES 15 1 C13440 20CU...

Page 94: ...C13440 20CU C13440 20CU01 Instruction manual_Ver 1 3 94 15 2 C13440 20CU01...

Page 95: ...the system You may be liable for repairs during the warranty period in the event of a natural disaster or if you handle the system contrary to the instructions in this manual use it without due cautio...

Page 96: ...p Local contact information worldwide can be found at www hamamatsu com l The contents of this manual are subject to change without notice l The unauthorized duplication or distribution of all or part...

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