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7

5 - prE-rInSIng

some liquids (e.g. protein-containing solutions and 

organic solvents) can leave a film of liquid on the 

inside the wall of the tip; pre-rinse the tip to minimize 

any errors that may be related to this phenomenon.

Pre-rinsing  consists  of  aspirating  the  first  volume 

of liquid and then dispensing it back into the same 

vessel (or to waste). subsequent volumes that you 

pipette  will  have  levels  of  accuracy  and  precision 

within specifications.

this pre-rinsing operation should be repeated when 

a new tip is used.

6 - pIpEtman dIamond

 

tIpS

PiPetman diamOnd tips are made to the highest 

specifications,  strict  quality  control  is  maintained 

throughout the manufacturing process. these tips 

are  used  to  calibrate  PiPetman Fixed,  therefore 

for  optimum  performance  we  recommend  using 

PiPetman diamOnd tips with your PiPetman Fixed. 

PiPetman  diamOnd  tips  have  the  Gilson  logo 

engraved on their collar, ensuring that you have a 

genuine  Gilson  product.  to  ensure  accuracy  and 

precision, Gilson's Quality assurance system focuses 

on the following critical parameters.

•  PIPETMAN 

diamOnd tips  are  made  from  pure 

polypropylene  (virgin,  metal-free,  to  avoid  the 

possibility of contamination). they are available 

sterilized and with filters.

•  Optimized  shape  (revised  collar  for  optimum 

sealing, thin walls, and fine point), making them 

easier to mount, more flexible, with no vortexing, 

and improved precision.

•  PIPETMAN 

diamOnd  tips  are  free  from  even 

microscopic defects, especially at the orifice. all 

surfaces  are  smooth  and  hydrophobic,  thereby 

avoiding the excessive retention of liquids that 

causes poor accuracy and a lack of precision.

Summary of Contents for Pipetman Fixed

Page 1: ...User s Guide...

Page 2: ...sable tipsmeannoneedtohandle tips protecting the user from contamination Thetip holder theconnect ing nut and the tip ejector are autoclavable for 20 minutes at 121 C and 0 1 MPa pressure CONTENTS pag...

Page 3: ...y guaranteed using genuine PIPETMAN Diamond Tips The data given in the table conform to the ISO 8655 2 Standard Maximum Permissible Errors Model Volume Gilson ISO 8655 Reference L Systematic Random Sy...

Page 4: ...n measurements for each test volume 3 OPERATION Place a tip on the tip holder Press the tip on firmly using a slight twisting motion to ensure a positive airtight seal See Chapter 6 for the appropriat...

Page 5: ...calibrated using distilled waterandveryhighprecisionbalances Thenominal valueofthePipetmanFixedmaybeslightlyadjusted to compensate for liquids of different density or viscosity Adjustmentofthefactoryc...

Page 6: ...n displayrepresents0 4 LfortheF100 1 10of4 L thecalibra tiontoolmustbeturned3unitsuntilthedisplayreads B0 2 Itispossibletoalterthevolumebymorethanonefull turn in the counterclockwise direction In this...

Page 7: ...rate Pipetman Fixed therefore for optimum performance we recommend using PIPETMANDiamondTipswithyourPipetmanFixed PIPETMAN Diamond Tips have the Gilson logo engraved on their collar ensuring that you...

Page 8: ...oided Gilson ssterilizedDiamondFilterTipsarecertifiedfree ofdetectableRNase DNase DNA RNA andprotease pipetman DiamondTipstouseforbestresults Gilson Logo ID Number Pipette PIPETMAN Diamond FilterTips...

Page 9: ...older To prevent this Press and release the push button slowly and smoothly Never turn the pipette upside down Never lay the pipette on its side when there is liquidinthetip Useadedicatedstandorhang e...

Page 10: ...bly Disassemblethepipetteandreassemblethepipette correctly Ifthereisliquidinthetip holder cleanthepipette as follows Remove the tip ejector Unscrew the connecting nutandrinsethetip holder piston seala...

Page 11: ...cekit1stlevel 1tip holder 3piston sealsand3O rings F144495 F144496 F144497 F144498 F144499 Adjustmentkey F123674 F123674 F123674 F123674 F123674 Bagsof10filters F161280 Bagsof100filters F161241 If a p...

Page 12: ...12...

Page 13: ...Directives Villiers le Bel October 1st 2010 GILSON S A S 19 avenue des Entrepreneurs BP 145 95400 VILLIERS LE BEL FRANCE Tel 33 0 1 34 29 50 00 Fax 33 0 1 34 29 50 20 PIPETMAN Fixed F2 F5 F10 F20 F25...

Page 14: ...14 NOTES...

Page 15: ...15...

Page 16: ...ed Gilson Inc World Headquarters 3000 Parmenter Street I P O Box 620027 Middleton WI 53562 0027 USA Tel 1 800 445 7661 or 1 608 836 1551 I Fax 1 608 831 4451 Gilson S A S 19 avenue des Entrepreneurs I...

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