10
8 - trouBLESHootIng
If there is a leak or the pipette is not accurate,
check for the following:
• The connecting nut is loose.
Tighten the connecting nut.
• The tip-holder is cracked or scored.
remove the tip ejector and inspect the tip-holder.
For F2 to F20 models, if the tip-holder is damaged,
the piston may also be damaged. replace the dam-
aged parts (see Chapter 9). When re-assembling the
pipette, the connecting nut should be hand tightened.
• Chemical damage to the piston and seals.
replace the piston and seals (see Chapter 9).
Wash
the inside of the tip-holder using distilled water.
• Improper reassembly.
Disassemble the pipette and reassemble the pipette
correctly.
If there is liquid in the tip-holder, clean the pipette
as follows:
• Remove the tip ejector. Unscrew the connecting
nut and rinse the tip-holder, piston, seal and O-ring
with distilled water. dry these parts and reassemble
the pipette.
If necessary, the tip-holder, connecting nut and the
tip ejector can be autoclaved for 20 minutes at 121°C
and at 0.1 mPa pressure. The seal and the O-ring
should not be autoclaved.
If there is an air bubble when the sample is
aspirated:
• Dispense the sample into its original vessel.
• Ensure that the tip is properly immersed in the
sample liquid.
• Aspirate the sample more slowly.
if the bubble appears a second time, replace the tip.
the outside of the PiPetman Fixed can be cleaned
using a cloth dampened with isopropanol.
