6
to adjust the volume setting to
compensate for a specific density
or viscosity, slide the key over the
push-button until notches fit into
the grooves in the calibration sleeve
(Figure 3), then turn the key:
• clockwise to decrease the volume;
• counterclockwise to increase the
volume; go above the desired
value, then decrease the volume
to reach the required setting.
Example
: When using a PIPeTmAn F100 to aspirate a par-
ticularly viscous solution, you may determine gravimetrically
that the volume delivered is 98.8 µL, and the display reads “e
0.2”. Therefore, you wish to increase the volume dispensed by
1.2 µL. As the interval between each letter on the calibration
display represents 0.4 µL for the F100 (1/10 of 4 µL), the calibra-
tion tool must be turned 3 units until the display reads “B 0.2”.
it is possible to alter the volume by more than one full
turn in the counterclockwise direction. in this case,
remember to readjust the same number of turns
when it is necessary to return to the original setting.
Check the new volume gravimetrically. if the volumes
delivered are still not sufficiently close to the desired
value, make another slight adjustment in the relevant
direction. Be sure to change tips between volume
setting adjustments and to pre-rinse each new tip.
When the
PiPetman Fixed is read-
justed to compensate for a particular
solution, the original setting (ex. 0.2)
can be noted on the self-adhesive
labels supplied (Figure 4).
Liquids of high density, vapor pres-
sure, or viscosity are best pipetted
by the Gilson mICrOmAn
®
positive
displacement pipette.
Figure 3
Figure 4
