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5

dispensing

•  Place the end of the tip against the inside wall of 

the vessel at an angle of 10 to 40 degrees. Press 

the push-button smoothly to the first stop (Fig. 

2C). Wait one second. Press the push-button to 

the second stop to expel any residual liquid from 

the tip (Fig. 2d).

•  Keep  the  push-button  pressed  fully  down  and 

(while removing the pipette) draw the tip along 

the inside surface of the vessel.

•  Release the push-button smoothly.
•  Eject the tip by pressing the tip-ejector button.

4 - uSEr adjuStmEntS

PiPetman Fixed is factory calibrated using distilled 

water and very high precision balances. the nominal 

value of the PiPetman Fixed may be slightly adjusted 

to  compensate  for  liquids  of  different  density  or 

viscosity.

Adjustment of the factory calibration must 
only be performed using a special calibra-
tion key (Figures 3 and 5G).

this adjustment to accomodate for density or viscos-

ity is limited to one full turn of the calibration key in 

either direction, which equals:
±  1.0 µL 

(models F2  to F20)

±  4.0 µL 

(models F25  to F100)

±  10.0 µL  (models F120  to F200)

±  40.0 µL  (models F250  to F1000)

±  200.0 µL  (model F5000).

The display is graduated in tenths of the key revolu-

tion (letters A, B,... J) and is read from left to right. each 

small graduation mark represents 0.2 of the distance 

between each letter.

Summary of Contents for Pipetman Fixed

Page 1: ...User s Guide...

Page 2: ...sable tipsmeannoneedtohandle tips protecting the user from contamination Thetip holder theconnect ing nut and the tip ejector are autoclavable for 20 minutes at 121 C and 0 1 MPa pressure CONTENTS pag...

Page 3: ...y guaranteed using genuine PIPETMAN Diamond Tips The data given in the table conform to the ISO 8655 2 Standard Maximum Permissible Errors Model Volume Gilson ISO 8655 Reference L Systematic Random Sy...

Page 4: ...n measurements for each test volume 3 OPERATION Place a tip on the tip holder Press the tip on firmly using a slight twisting motion to ensure a positive airtight seal See Chapter 6 for the appropriat...

Page 5: ...calibrated using distilled waterandveryhighprecisionbalances Thenominal valueofthePipetmanFixedmaybeslightlyadjusted to compensate for liquids of different density or viscosity Adjustmentofthefactoryc...

Page 6: ...n displayrepresents0 4 LfortheF100 1 10of4 L thecalibra tiontoolmustbeturned3unitsuntilthedisplayreads B0 2 Itispossibletoalterthevolumebymorethanonefull turn in the counterclockwise direction In this...

Page 7: ...rate Pipetman Fixed therefore for optimum performance we recommend using PIPETMANDiamondTipswithyourPipetmanFixed PIPETMAN Diamond Tips have the Gilson logo engraved on their collar ensuring that you...

Page 8: ...oided Gilson ssterilizedDiamondFilterTipsarecertifiedfree ofdetectableRNase DNase DNA RNA andprotease pipetman DiamondTipstouseforbestresults Gilson Logo ID Number Pipette PIPETMAN Diamond FilterTips...

Page 9: ...older To prevent this Press and release the push button slowly and smoothly Never turn the pipette upside down Never lay the pipette on its side when there is liquidinthetip Useadedicatedstandorhang e...

Page 10: ...bly Disassemblethepipetteandreassemblethepipette correctly Ifthereisliquidinthetip holder cleanthepipette as follows Remove the tip ejector Unscrew the connecting nutandrinsethetip holder piston seala...

Page 11: ...cekit1stlevel 1tip holder 3piston sealsand3O rings F144495 F144496 F144497 F144498 F144499 Adjustmentkey F123674 F123674 F123674 F123674 F123674 Bagsof10filters F161280 Bagsof100filters F161241 If a p...

Page 12: ...12...

Page 13: ...Directives Villiers le Bel October 1st 2010 GILSON S A S 19 avenue des Entrepreneurs BP 145 95400 VILLIERS LE BEL FRANCE Tel 33 0 1 34 29 50 00 Fax 33 0 1 34 29 50 20 PIPETMAN Fixed F2 F5 F10 F20 F25...

Page 14: ...14 NOTES...

Page 15: ...15...

Page 16: ...ed Gilson Inc World Headquarters 3000 Parmenter Street I P O Box 620027 Middleton WI 53562 0027 USA Tel 1 800 445 7661 or 1 608 836 1551 I Fax 1 608 831 4451 Gilson S A S 19 avenue des Entrepreneurs I...

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