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ASPEC® PoSitivE PrESSurE MAnifold
|
User's GUide
USER’S GUIDE
|
ASPEC™ POSITIVE PRESSURE MANIFOLD
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Troubleshooting Table
Symptoms
Possible Causes
SPE Steps
Solutions
Lack of flow
No inlet pressure.
All
Check that the gas pressure source is set to 15 psi.
O-ring damaged or missing.
Replace the O-ring. Refer to
page 31
.
Damaged process control dial.
Replace the front panel. Contact your local Gilson representative. Refer to
page 36
.
Liquid flows unevenly
Channeling inside the sorbent
of the SPE cartridge/s.
Condition
Specific Causes
Specific Solutions
Drying of the cartridge/s.
Repeat the condition step.
Insufficient condition volume.
Increase the condition volume.
Excessive flow rate.
Reduce the flow rate.
Clogged SPE cartridge/s.
Condition
Replace the SPE cartridge/s.
Load
Reconsider the sample preparation workflow; e.g., sample filtration,
centrifugation, etc.
Heterogeneous viscosity
inside the SPE cartridge/s,
caused by the use of
different liquids in successive
SPE steps.
Condition
Reduce the residual level of liquid above the frit to 1 to 2 mm before
conditioning with another type of liquid.
Wash and
Elute
Before using a new solvent, ensure that all effluent has been expelled from
the SPE cartridges.
Leak in the manifold gasket.
All
Replace the manifold gasket. Refer to
page 31
.
Liquid continues to flow,
even after the SPE control
dial has been set to
off
.
The process control dial is set
to
dry
.
All
Switch the process control dial to
SPE
.
Residual positive
pressure remains in the
SPE cartridge/s.
Switch the SPE control dial to
low flow
before stopping. Unseat the
manifold. Refer to
page 24
.
