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ASPEC® POSITIVE PRESSURE MANIFOLD
|
USER'S GUIDE
O
PERA
TION
General Flow Guidelines
The following table provides general process and flow guidelines.
Table 1
SPE Process and Control Guidelines for SPE Steps
SPE Steps
Process
Control
Setting
SPE Control Setting
NOTE
For all low and high flow recommendations, refer to the
SPE Format Flow Guidelines table below.
Condition
SPE
High Flow, and then switch to Low Flow
Load
SPE
Low Flow, based on viscosity
Wash
SPE
High Flow, based on viscosity
Dry
Dry
Off
Elute
SPE
Low Flow, based on viscosity
Table 2
SPE Format Flow Guidelines
SPE Format
High Flow
(drops per second)
Low Flow
(drops per second)
6 mL
3 to 5
1
3 mL
1
1 drop per 2 to 3 seconds
1 mL
microplate
0.5 to 1
1 drop per 3 to 4 seconds
