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ASPEC® Positive Pressure Manifold

User's Guide

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Summary of Contents for ASPEC

Page 1: ...ASPEC Positive Pressure Manifold User s Guide...

Page 2: ...This Page Is Intentionally Left Blank...

Page 3: ...ll rights reserved All product and or company names are trademarks of their respective holders Use of the trademark s in this document does not imply any affiliation with or endorsements by the tradem...

Page 4: ...7 Signs of Damage 7 Site Requirements 7 Storage and Movement 7 S curit 8 Symboles et Notes 8 Risques Chimiques 9 Solvants Inflammables 9 Pi ces de Remplacement 9 Signes de D t rioration 9 Exigences du...

Page 5: ...uidelines 27 Basic SPE Technique 28 Maintenance 31 Clean the ASPEC PPM 31 Install or Replace the O Ring 31 Replace the Manifold Gasket 31 Maintenance Parts 31 Troubleshooting Table 32 Repair and Retur...

Page 6: ...andards of design manufacture and intended use of the instrument Gilson assumes no liability for customers failing to comply with these requirements Refer to the rear panel label on the instrument for...

Page 7: ...e not exposed to hazardous levels of toxic substances as outlined in the material safety data sheets MSDSs or any documentation provided by local governing bodies such as The Health Protection Agency...

Page 8: ...uide compromet les normes de s curit de conception de fabrication et d utilisation pr vue de l instrument Gilson d cline toute responsabilit en cas d incapacit du client se conformer ces exigences Mer...

Page 9: ...indiqu dans les fiches de donn es de s curit fDS ou toute documentation fournie par les instances locales telles que l agence de protection de la sant Royaume Uni ou l administration de la s curit et...

Page 10: ...also an ideal choice for SPE cleanup prior to LCMS for bioanalyses Drain Well Refer to page 12 for a detailed description SPE Racks 15 24 and 96 positions available Figure 1 Standard Components of th...

Page 11: ...PE control dial Refer to page 13 Low Flow Adjustment Knob Used to fine tune flow when Low Flow is selected on the SPE control dial Refer to page 13 Manifold Positioning Lever Raises and lowers the man...

Page 12: ...paration solvents and matrix to waste Ensure that the waste outlet tubing flows to a large waste container Ejection Plate Used to quickly eject all the SPE cartridges from the cartridge rack Slide the...

Page 13: ...e the valve and stop flow NOTE The loss of tension in the adjustment knobs indicates that the valve is fully closed Flow Adjustment Locks Used to lock in flow adjustments made with the low and high fl...

Page 14: ...Symbol unlocked and pull the lever forward to lock Green Lock Symbol locked Manifold Positioning Lever Raises and lowers the manifold over the SPE cartridge holder The manifold locking lever must be...

Page 15: ...he operating pressure of the system which should not exceed 15 psi SPE Cartridge Plugs Optional Used to fill empty positions in the SPE cartridge racks 15 and 24 position Figure 11 Pressure Gauge Plug...

Page 16: ...a quality control checklist 3 Read the user s guide 4 Remove the box containing the standard equipment and accessory kit and then set it aside 5 Check that all items specific to the configuration orde...

Page 17: ...Tygon 0 25 OD 3 ft qty 1 Documentation ASPEC Positive Pressure Manifold User s Guide qty 1 USB drive with video of a sample protocol qty 1 Configuration Kit 96 Position SPE Manifold Kit PN 37417014 Ga...

Page 18: ...L qty 1 Gasket with adhesive backed seal 24 positions qty 2 Manifold SPE 24 positions qty 1 O ring manifold seal qty 1 Tube rack SPE collection 1 3 mL 12 x 75 mm qty 1 Recommended Accessories All Conf...

Page 19: ...D x H 33 x 48 3 x 44 2 cm 13 x 19 x 17 4 in Liquid contact materials Description Material Gasket Neoprene Locator plate Ertalyte Site requirements Specification Definition Environment Indoor use only...

Page 20: ...ining clip aside 4 If necessary remove the pre installed manifold NOTE Ensure that the O ring does not fall out while removing the manifold 5 Slide the new manifold into the manifold carrier with the...

Page 21: ...he instrument NOTE The orange plunger insert will automatically engage the tubing Pull back the tubing to check for proper engagement Depress the orange plunger insert to release the tubing NOTICE Ens...

Page 22: ...r Flow to Waste 1 Slide the locator plate to the front of the locator bed 2 Place the drain well on the front position of the locator plate 3 Connect the waste outlet tubing to the barbed fitting on t...

Page 23: ...well to the rear position of the locator plate 3 Place the 15 or 24 position tube rack with tubes on the front position of the locator plate 4 Set the appropriate 15 or 24 position SPE cartridge rack...

Page 24: ...with the tops of the SPE cartridges 5 Pull the manifold locking lever forward to the fully locked position Green Lock Symbol locked to create a seal between the manifold and the SPE cartridges NOTICE...

Page 25: ...tment knobs counterclockwise to reduce flow and close the valve The loss of tension in the adjustment knobs indicates that the valve is fully closed High Flow Recommended for SPE cartridge wetting dur...

Page 26: ...e flow reaches 1 to 2 drops per second 4 To turn off low flow switch the SPE control dial to Off Dry Recommended for drying the SPE cartridges with maximum flow To turn on the dry setting 1 Ensure the...

Page 27: ...r all low and high flow recommendations refer to the SPE Format Flow Guidelines table below Condition SPE High Flow and then switch to Low Flow Load SPE Low Flow based on viscosity Wash SPE High Flow...

Page 28: ...he desired flow rate is achieved 7 When the solvent levels decrease to 2 mm above the fastest eluting cartridge of any one of the SPE cartridge s quickly switch the SPE control dial to Low Flow 8 Turn...

Page 29: ...to Low Flow 15 Turn the low flow adjustment knob clockwise until the desired flow rate is achieved 16 When the sample stops dripping from the SPE cartridges turn the SPE control dial to Off 17 Unseat...

Page 30: ...the appropriate volume of eluent into each available SPE cartridge 30 Switch the SPE control dial to Low Flow 31 Turn the low flow adjustment knob clockwise until the desired flow rate is achieved 32...

Page 31: ...manifold Replace the Manifold Gasket 1 Remove the manifold from the manifold carrier 2 Set the manifold down with the gasket facing up 3 Peel the gasket away starting from the corner Discard the gaske...

Page 32: ...low rate Reduce the flow rate Clogged SPE cartridge s Condition Replace the SPE cartridge s Load Reconsider the sample preparation workflow e g sample filtration centrifugation etc Heterogeneous visco...

Page 33: ...estarts and the liquid level equilibrates for all cartridges Not enough time for extraction to be completed Load Decrease the flow rate to better extract analytes from the sample matrices Elution Decr...

Page 34: ...n Serial number and model number of the instrument located on the rear of the base List of concise symptoms List of operating procedures and conditions you were using when the problem arose Warranty R...

Page 35: ...son representative for damage caused by improperly packaged instruments Indicate the authorization on the carton and on the packing slip Always insure for the replacement value of the unit Include a d...

Page 36: ...ain well qty 1 37117000 Ejection plate for SPE cartridges qty 1 37114006 External gas regulator 15 psi qty 1 37117100 Gasket manifold 96 positions qty 10 37417005 Gasket manifold 24 positions qty 10 3...

Page 37: ...onfiguration Kits Configuration Kits Description Part Number 96 position 37417014 24 position 1 mL 37417011 24 position 3 mL 37417012 15 position 6 mL 37417013 ASPEC Positive Pressure Unit Description...

Page 38: ...LT380170 01 www gilson com ContactUs...

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