GE FineLINE 35 oligo, Operating Instructions Manual

Page: 9 / 44

FineLINE 35 oligo column Operating Instructions 28-9649-57 AA
9
Introduction 1
Column description 1.3
1.3 Column description
Usage
FineLINE 35 oligo column is intended for method development at pilot scale or small
scale production of oligonucleotides.
It is designed for maximum scalability and coupling efficiency using Primer Support™
200. The column is easy to operate.
Design
The FineLINE 35 oligo column design incorporates a number of features.
The FineLINE 35 oligo column has a simple design. It comprises a number of complete
units, including a hydraulically or manually adjustable adapter and special nets in the
end-piece and adapter, which are easily assembled together.
The column tube is held in place between the top end-piece and bottom end-piece by
three support rods.
The top endpiece houses the hydraulically adjustable adapter. The adapter contains a
filter unit with a stainless steel (316L) filter.
The adapter is held in place by a mechanical locking unit on the adapter piston. The
adapter lid is secured to the top end-piece with a bayonet fitting. O-rings seal the lid
against the adapter rod and top end-piece. The adapter is sealed against the column
with two O-rings.
The bottom end-piece contains the bottom filter unit and stainless steel (316L) filter and
an O-ring seals the unit against the column tube.
Part Description
1 Column top connection
2 Adapter shaft
3 Mechanical locking unit
4 Bayonet adapter lid lock
5 Column top lid
6 Connecting rod
7 Column tube
8 Adapter filter unit
9 Bottom filter unit
10 Bottom lid with bottom
connection
11 Hydraulic chamber connection
1
2
11
6
5
7
8
10
3
4
9