GE FineLINE 35 oligo, Operating Instructions Manual

Page: 10 / 44

1 Introduction
1.3 Column description
10
FineLINE 35 oligo column Operating Instructions 28-9649-57 AA
Materials
All column parts are manufactured from high quality materials and meet the
requirements of industrial environments. The wetted parts are manufactured from
materials having high chemical resistance.
The materials used in the construction of the FineLINE 35 oligo column have been
carefully selected to be compatible with reagents commonly used in oligonucleotide
synthesis.
Materials of manufacture of FineLINE 35 oligo column include borosilicate glass, PEEK™,
polypropylene, stainless steel, Simriz. PFR91 and Teflon™ (PTFE).
In the current FineLINE 35 oligo column the top end-piece and adapter lid are made of
PEEK. Previous versions have these parts made of POM. PEEK is necessary only if ACN is
to be used in the hydraulic chamber. If ACN is to be used in old versions of the column,
PEEK-parts can be ordered and exchanged,
Section 7.4 Ordering information‚ on
page 38 .
PEEK parts are labelled P to be distinguished from POM parts.
The column material is not compatible with tetrahydrofurane, THF.
Connections
Located on the top end-piece is the hydraulic inlet, complete with a check valve, and
hydraulic outlet.
The inlet and outlet of the column are located at the bottom of the column, in the
bottom lid, and in the column top.
The inlet and outlets have 5/16” fitting and the hydraulic chamber has M6 fitting.