Corrective action
Possible cause
Error symptom
Check that the pump is operating
and is programmed correctly.
Incorrect pump func-
tion
Waves on the gradi-
ent
Check that the mixing chamber is
free from dirt or particles.
Dirty mixing chamber
Change to a larger mixing cham-
ber volume if necessary.
Insufficient mixing
chamber volume
Check the motor operation. Place
a hand on the mixer and start it by
starting the pump at a low flow
rate. You should both hear and feel
the mixer motor and stirrer when
they are spinning.
Incorrect motor func-
tion
Check for loose tubing connec-
tions. Use the flow restrictor.
Air in the flow cell
Ghost peaks appear
in the gradient profile
Wash the tubing and check pump
is operating properly.
Dirty tubing
Unlinear gradients or
slow response to %B
changes
Change to smaller mixer volume.
Incorrect mixer vol-
ume
ÄKTAprime plus Operating Instructions 28-9597-89 AC
51
6 Troubleshooting
6.2 Conductivity curve problems
