Corrective action
Possible cause
Error symptom
Check the lamp run time according
to and replace if necessary. Refer
to
ÄKTAprime plus User Manual
.
Aging UV lamp
Low sensitivity
Check that the lamp position and
the filter position are both set to
the wavelength to be used, 280 nm
or 254 nm. Refer to
ÄKTAprime plus
User Manual
.
UV lamp in wrong po-
sition
Calculate the theoretical extinction
coefficient of the protein. If it is ze-
ro or very low at 280 nm, the pro-
tein cannot be detected.
The theoretical extinc-
tion coefficient too
low
6.2
Conductivity curve problems
Corrective action
Possible cause
Error symptom
Check the flow restrictor after the
flow cell.
Air in the pump or the
flow cell
Baseline drift or noisy
signal
Tighten the clamps. If necessary,
replace the clamps.
Leaking tube connec-
tions
Check the mixer function by plac-
ing a stirrer bar on top of the mixer
housing. The stirrer bar should ro-
tate when the system is in
Run
mode. The mixer function can also
be checked by running the installa-
tion test.
Incorrect mixer func-
tion
Clean the conductivity cell by
flushing 1 M NaOH or 20% ethanol.
Dirty conductivity cell
Equilibrate the column. If neces-
sary, clean the column using a
method plan for column cleaning.
Column not equilibrat-
ed
Clean the flow cell according to
procedure in
Monitor User Manual
.
Dirty flow cell
Conductivity measure-
ment with the same
buffer appears to de-
crease over time
Use a temperature compensation
factor. See
Monitor User Manual
.
Decrease in ambient
temperature
50
ÄKTAprime plus Operating Instructions 28-9597-89 AC
6 Troubleshooting
6.1 UV curve problems
