4
Check that the tube sensor (1) is in the correct position for the tube size. The eluent
tubing should be over the center of the collection tube. Use the red sensor control
knob (2) to position the tube holder (3).
2
1
3
5
Rotate the rack by hand until the rear half of the tube sensor rests against the first
tube.
6
Press
feed tube
on the front panel (see
Figure 1.2
). The bowl moves to the correct
position to collect the first fraction in the first tube.
7
Make sure that drop synchronization is turned on.
Drop synchronization can NOT be used at flowrates above 3 ml/min.
Note:
Preparing the monitors
1
Check the UV lamp filter position and the lamp position.
2
Calibrate the pH electrode (optional).
See
ÄKTAprime plus User Manual
for more information.
Filling the buffer inlet tubing
When running an application templates, the buffer inlet tubing will automatically be
filled with buffer.
For other applications, fill the inlet tubing manually with buffer as described in the
ÄKTAprime plus User Manual
.
Filling the sample loop
Using an injection fill port
ÄKTAprime plus Operating Instructions 28-9597-89 AC
37
4 Operation
4.3 Preparations before start
