2
Press
OK
. The switch valve turns to the inlet
B
port.
3
Draw buffer with the purge syringe until liquid enters the syringe.
4
Replace the purge tubing with the stop plug.
5
Stop the pump by pressing
end
and then
OK
.
Flushing the pump with 100% ethanol:
1
Put inlet tubing
A1
in deionized water.
2
Run the pump at 40 ml/min for 1 min and press
pause/cont
.
3
Move inlet tubing
A1
to 100% ethanol
4
Press
pause/cont
, run the pump for 10–20 s and press
pause/cont
.
5
Set the flow rate to 5 ml/min using the arrow buttons.
6
Press
pause/cont
, run the pump for at least 30 s and press
pause/cont
.
7
Move inlet tubing
A1
to deionized water.
8
Press
pause/cont
and run the pump for 1 min.
9
Finish by pressing
end
and then
OK
.
Preparing the tubing and column
1
Put inlet tubing
A1
in the binding buffer.
2
Put inlet tubing
B
in the elution buffer.
3
Put the three waste capillaries (brown color) from port
4
and
5
on the injection valve
and port
NO
on the fraction collector valve in waste.
4
Connect a column, for example a HisTrap™ HP 1 ml column, between port
1
on the
injection valve and the upper port of the UV flow cell. Use a suitable length of PEEK
tubing and 1/16" male connectors.
1
2
3
4
ÄKTAprime plus Operating Instructions 28-9597-89 AC
35
4 Operation
4.3 Preparations before start
