6
Choose to wash the
A2
–
A8
inlet tubing that is used by pressing
OK
at those cursor
positions.
A1
and
B
will always be washed.
At delivery, only
A1
and
B
are installed.
Note:
7
Scroll to
OK
and press the
OK
button.
8
Press
OK
to start the method.
9
When the method is finished, replace the first collection tube. It will contain a small
amount of water after the system wash.
Purging the pump and inlet tubing
If there are large amounts of air in the tubing or if you suspect air in the pump, use the
Purge kit to purge the flow path. Air bubbles that still are trapped in the pump (causing
increased pulsation) can be removed by flushing 100% ethanol through the pump. These
two procedures are described in the following two sections.
Purging the flow path using the Purge kit:
1
Remove the stop plug from the pump.
2
Connect the Purge kit to the pump.
1
2
3
4
5
6
7
8
9
10
ml
3
Put the used inlet tubing in the appropriate buffers.
4
Run the pump at 0.1 ml/min.
Filling inlet tubing
A1
–
A8
:
1
Go to
Set Buffer Valve
using the arrow buttons.
2
Set the chosen
A
inlet and press
OK
. The valve switches to the selected port.
3
Draw buffer with the purge syringe until liquid enters the syringe.
4
Repeat step 1–3 until all chosen
A
inlet tubing is filled.
Filling inlet tubing
B
:
1
Go to
Set Concentration %B
and set the concentration to
100%
.
34
ÄKTAprime plus Operating Instructions 28-9597-89 AC
4 Operation
4.3 Preparations before start
