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FujiFilm FLA-8000, Operation Manual

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FujiFilm FLA-8000 Operation Manual Download Page 8

2002. Jan. Verson 3.0

6

The FLA-8000 uses the following three lasers:
(1) LD laser, class IIIb, wavelength of 635 nm, maximum power of 20 mW (CW)
(2) SHG laser, class IIIb, wavelength of 532 nm, maximum power of 5.5 mW (CW)
(3) SHG laser, class IIIa, wavelength of 473 nm, maximum power of 2 mW (CW)

(Optional)

This instrument meets the laser radiation safety requirements specified in the Code
of Federal Regulations (21 CFR, Chapter 1, Subchapter J).

It also conforms to EN60825-1/A11 (1996), Class 1 Laser Product.

2. Laser Safety

Lasers used

Applicable safety standards

This instrument conforms to the above-mentioned standards and is designed to be
safe against laser radiation.  The laser leak level is suppressed below 22.1 mJ for
sufficiently safe operation, provided the user carries out operation (including user
maintenance) of the instrument properly.

The cover is fixed with screws to the instrument.  If you loosen these screws or
detach the cover, laser beam may leak out.  Never loosen these screws or detach
the cover.  Such an act is allowed to a serviceman only.

Laser safety

Instrument cover

Never detach the inner cover screwed to this instrument.  If it is
detached, laser beam may leak and you may loose your eye-
sight.

 

WARNING

2. Laser Safety

Summary of Contents for FLA-8000

Page 1: ...FLA 8000 OPERATION MANUAL 2002 Jan Version 3 0 Version 3...

Page 2: ......

Page 3: ...rd Prevention Related to Radioisotope Pollution 10 Controlled area 10 Limit of superficial pollution 10 Installation site of instrument 10 Carrying out of the controlled area 10 7 Radioisotope RI Poll...

Page 4: ...s 54 Part 6 Scanning IPs 57 Part 7 Scanning Gel Samples 77 Part 8 Setting Optional Filters 101 Part 9 Daily Maintenance 105 1 Maintenance of SHG Laser 106 Part 10 Troubleshooting 107 Troubles in FLA 8...

Page 5: ...2002 Jan Verson 3 0 3 Part 1 PRECAUTIONS...

Page 6: ...rocution Do not place a heavy object on this instrument If it falls down or drops it may cause injury WARNING CAUTION Do not modify this instrument Unauthorized modification may cause a fire electric...

Page 7: ...4 Preventing lightning damages This instrument requires class D grounding Class D grounding prevents electric shocks or electrocution in the case where electric current leaks from an instrument whose...

Page 8: ...sed Applicable safety standards This instrument conforms to the above mentioned standards and is designed to be safe against laser radiation The laser leak level is suppressed below 22 1 mJ for suffic...

Page 9: ...2002 Jan Verson 3 0 7...

Page 10: ...detach the cover you may pos sibly get an electric shock or be electrocuted in the worst case This instrument fits or conforms to the regulations and standards shown below Standard List EMC Japan VCCI...

Page 11: ...ple condition and it is quite difficult to foresee the degree of pollution Radioisotope pollution may harm the FLA 8000 body due to improper operation or an unexpected trouble The performances of the...

Page 12: ...s Non RI method Therefore it is recommended that the user should install it outside the controlled area and use RI indicated samples without contact ing them with IPs directly When a sample is in dire...

Page 13: ...pollution may be tran scribed to the non polluted IPs Be very careful IPs may be used repetitively and accordingly should be protected against RI pollu tion If an RI sample is used in direct contact w...

Page 14: ...irect sunlight which is not suitable to IP reading and may cause temperature rise inside the instrument or shield leak age resulting in a failure of proper operation of the instrument Install the inst...

Page 15: ...ION The slide carrier weighs 420 g If it drops onto your feet your feet will be injured The slide carrier consists of precision parts Handle it with great care If it drops or impact or external force...

Page 16: ...drop it Never put any objects other than samples on the glass or apply pressure to the glass Be very careful not to break the gel carrier glass Broken glass may cause injury The gel carrier with the...

Page 17: ...3 0 15 IP Carrier The IP carrier weighs 370 g If it drops onto your feet your feet will be injured The IP carrier becomes unusable if it drops and deforms or its ends are lost IP Carrier 11 Other Inst...

Page 18: ...2002 Jan Verson 3 0 16...

Page 19: ...2002 Jan Verson 3 0 17 Part 2 SYSTEM CONFIGURATION...

Page 20: ...ture saves costs Besides compactly designed IPs are easier to handle than the traditional X ray film With these features a single FLA 8000 enables not only gel imaging of DNA RNA and protein but also...

Page 21: ...to the analyzer unit Hereinafter the instrument body is merely referred to as the body in this manual 2 Standard System Configuration 2 AC Power Cable The AC power cable is exclusive for this instrum...

Page 22: ...ded in the accessories mount the IP filter and set the folder to the instrument 7 Reading Software Image Reader Install Image Reader in the analyzer unit and set the reading conditions to control the...

Page 23: ...2002 Jan Verson 3 0 21 Part 3 Switching On and Off...

Page 24: ...ions as shown below Operation Procedures Switching On and Off the FLA 8000 Switching On Switching Off Operation Procedures Immediately after the FLA 8000 is switched on The POWER lamp goes on During s...

Page 25: ...2002 Jan Verson 3 0 23 Part 4 Scanning Slide Glass Samples...

Page 26: ...on the PC 3 Make sure that warming up is completed and the FLA 8000 is ready Activate the FLA 8000 Image Reader from the Startup menu or using the shortcut key 4 The main window of the FLA 8000 Image...

Page 27: ...side fully to the very end through the opening of the slide glass carrier Note In an image scanned by the FLA 8000 the slide numbers on the slide glass carrier are displayed in the upper part of the m...

Page 28: ...2002 Jan Verson 3 0 26 2 3 Open the sample setting door 2 4 Open the carrier locking cover Scanning Slide Glass Samples Carrier Set Mode...

Page 29: ...oles in the carrier 2 6 Close the carrier locking cover firmly Close the carrier locking cover so that the poles on both sides of the carrier locking cover are securely engaged with the pole holds on...

Page 30: ...oor 3 Go back to the PC and click on Setting in the PC window On the right of Carrier Set in the upper part of the window or lower left of the window 4 The window for setting the scanning conditions a...

Page 31: ...scanning mode speed Std Fast Scans pixels at 400 mm second High Sens Slow Scans pixels at 200 mm second The sensitivity is higher at the slower scanning speed 1 3 Slide glass selection as well as las...

Page 32: ...nations are as shown below 635 mm 675 DF 20 Optional filter added 532 mm 570 DF 20 675 DF 20 Optional filters added 473 mm 530 DF 20 570 DF 20 675 DF 20 Optional filters added See page 99 for the met...

Page 33: ...Integers only b Dragging the mouse The white area is the scannable area and the red frame area is the scan ning range Drag the red frame area with the mouse to vary the scanning range As the red frame...

Page 34: ...background components in the slide glass may be reduced in the confocal mode On the contrary unevenness on the slide glass surface may be detected Non Confocal Mode The apparent evenness of images is...

Page 35: ...and high voltage condition Then click the PreView button 2 2 Preview starts in set conditions In the Preview mode the pixel size is larger and the reading speed is higher than in the final reading 2 3...

Page 36: ...ng three lasers the FLA 8000 pre scans the sample with these lasers twice each amounting to six times in all 2 4 On completion of PreView the window shown below appears Click the change over button to...

Page 37: ...Drag the adjuster button below PMT HV The density of the PreView image changes as the adjuster button is dragged Adjust the high volt age to be applied to the PMT with reference to this image density...

Page 38: ...dition in the pull down menu of the File menu Adialog box opens Input an intended name in the dialog box and save the file Loading the scanning conditions Select Load Condition in the pull down menu o...

Page 39: ...rt final scanning Scan Mode 1 Slide glass being scanned is marked with Scanned images are displayed in the window in sequence 2 Image window in the Scan mode There are several methods to display the s...

Page 40: ...2002 Jan Verson 3 0 38 Cascade Displays overlaid images Tile Horizontal Displays images horizontally Scanning Slide Glass Samples Scan Mode...

Page 41: ...2002 Jan Verson 3 0 39 Tile Vertical Displays images vertically Arrange Icons Windows illustrated as title bars are arranged Scanning Slide Glass Samples Scan Mode...

Page 42: ...og is selected on the Window menu the minimized catalog window is enlarged and displayed in the forefront 4 Change images scanned by three types of lasers in the slide glass window as shown below Scan...

Page 43: ...by the blue 473 nm laser Blue red Image scanned by the blue 473 nm laser overlaid on the image scanned by the red 635 nm laser Red green blue Images scanned by the three lasers all overlaid Click on t...

Page 44: ...displayed image click the button and click the mouse button in a position to be contracted in the image window To move a displayed image without changing the magnification click the button and drag th...

Page 45: ...n the histogram or by dragging the button below the histogram 6 Click on the Save icon in the Scan mode window or the Save button in the lower left The Scan mode window changes into the Save mode wind...

Page 46: ...ciple xxx 1_473 Click the button The Preference dialog box shown below is displayed Automatic Suffix Rule No Automatic file naming is not executed The operator should input a file name of each image f...

Page 47: ...hat character string and the suffix If you input test in the Base Name box for example the file name test1_473 is given to the first one of images scanned by the 473 nm laser Yes repetitive Automatic...

Page 48: ...IFF file Conversion setting Fixed Images are automatically converted into the TIFF file format using the algorithm in the software based on the maximum value of the images Custom Input a numeric value...

Page 49: ...his box is saved together with the image data 5 Selecting an image to be saved The file will be saved as long as the check box on the left side of the slide icon is chosen Deselecting the check box by...

Page 50: ...2002 Jan Verson 3 0 48...

Page 51: ...2002 Jan Verson 3 0 49 Part 5 Usage of IPs...

Page 52: ...it into electric signals A radiation image recorded on the IP during exposure is read as digital image information at the maximum resolution of 10 um per pixel 100 pixels mm and recorded in the analyz...

Page 53: ...vironmental conditions Do not expose IPs to the direct sunlight Do not use them in a place with much ultraviolet rays or natural radiation Do not use them in a hot and humid place Storage Store IPs le...

Page 54: ...age quality is guaranteed when an IP is scanned by the FLA 8000 immediately after it is exposed Therefore it is important to set the exposure start time and exposure time so that exposure ends just be...

Page 55: ...d completely In such a case scan it once by way of trial to check if it is erased completely Refer to the manual of the IP eraser The IP eraser may erase an IP in fourteen minutes if it is not exposed...

Page 56: ...ideoftheIPscanned first i e left side when the IP is set in the FLA 8000 and expose it Partial exposure reduces the scanning time 3 Set the IP on the cassette so that its exposure surface is in contac...

Page 57: ...eful not to apply an impact to the cassette An impact applied to the cassette may shift the sample and IP Do not expose the IP in a place with much environmental radiation in order to avoid the increa...

Page 58: ...2002 Jan Verson 3 0 56...

Page 59: ...2002 Jan Verson 3 0 57 Part 6 Scanning IPs...

Page 60: ...and Software Setup of Hardware and Software 1 Switch on the FLA 8000 and peripheral devices 2 Switch on the PC 3 Make sure that the FLA 8000 has warmed up and is ready for operation Activate the FLA...

Page 61: ...e Click on the IP icon or the radio button on the left of the IP icon to select the IP 2 Set the IP on the IP carrier 2 1 Put the IP carrier with the magnet surface upside as shown below Not selected...

Page 62: ...3 Turn down the room lighting below 20 luxes Keep the lighting turned down until the IP carrier is set and the main cover is closed 2 4 Take the IP out of the IP cassette and set it on the IP carrier...

Page 63: ...llowing figure shows the relationship between the IP setting position and the image position on the monitor When the IP is viewed from the back as shown below scanning starts at the upper left corner...

Page 64: ...7 Set the IP carrier Set the IP carrier so that the large hole for removing the IP in the carrier is located on the front side and the poles on the FLA 8000 are engaged with the holes in the carrier S...

Page 65: ...irmly Close the carrier locking cover so that the poles on both sides of the cover are securely engaged with the pole holds on the FLA 8000 The pole holds are located on the right and left 2 9 Close t...

Page 66: ...4 The window for setting the scanning conditions appears Setting Mode 1 The following shows the details of the scanning conditions 1 1 Resolution um Resolution Select a pixel scanning size The scanni...

Page 67: ...nal FUJI B390 filters for scanning IPs in the FLA 8000 before scanning an IP The FLA 8000 Image Reader does not automatically check whether the B390 filters are set If they are not set properly no ima...

Page 68: ...specified conditions 2 2 Root Folder Option The folder where the scanned data file is stored is displayed in the box below Root Folder Option If you want to change the folder click the button and chan...

Page 69: ...File menu A dialog box opens Input an intended name in the dialog box and save the file Loading the scanning conditions Select Load Condition in the pull down menu of the File menu A dialog box opens...

Page 70: ...displayed in the main window and the small window in the display area as soon as it is scanned 2 You may execute the following in the Scan mode 2 1 Skipping or stopping scanning Stop Click this butto...

Page 71: ...displayed image click the button and click the mouse button in a position to be enlarged in the image window To contract a displayed image click the button and click the mouse button in a position to...

Page 72: ...you want to change the gradation of displayed images select a scale in the pull down menu and adjust the gradation with the adjuster bar on the histogram or by dragging the button below the histogram...

Page 73: ...n the Save mode window 1 1 Giving an image file name It is possible to give names to image files automatically in a certain rule Serial numbers are added to the end of image file names Each file name...

Page 74: ...Automatic file naming is not executed The operator should input a file name of each image file Yes continuative Automatic file naming is executed Suffices are added to file names Select the suffix ty...

Page 75: ...sts of that character string and the suffix If you input test in the Base Name box for example the file name test1_640 is given to the first one of images scanned by the 640 nm laser Yes repetitive Au...

Page 76: ...into a TIFF file Conversion setting Fixed Images are automatically converted into the TIFF file format using the algorithm in the software based on the maximum value of the images Custom Input a numer...

Page 77: ...e Name box The comment input in this box is saved together with the image data 5 Cancelling your saving action If you do not want to save the IP image file read out deselect the check box next to the...

Page 78: ...2002 Jan Verson 3 0 76...

Page 79: ...2002 Jan Verson 3 0 77 Part 7 Scanning Gel Samples...

Page 80: ...1 Switch on the FLA 8000 and peripheral devices 2 Switch on the PC 3 Make sure that the FLA 8000 has warmed up and is ready for operation Activate the FLA 8000 Image Reader from the Startup menu or u...

Page 81: ...Set Mode 1 Select Gel in Select Sample in the Carrier Set mode Click on the Slide icon or the radio button on the left of the Slide icon to select the gel 2 Set gel on the gel carrier 2 1 Place the ge...

Page 82: ...The relationship between the gel setting position and the scanned image dis played on the monitor is as shown below Note Be careful not to make the gel buffer solution exceed the gel stopper inside th...

Page 83: ...2002 Jan Verson 3 0 81 2 3 Open the sample setting door 2 4 Open the carrier locking cover Scanning Gel Samples Carrier Set Mode...

Page 84: ...cover is located on the left and the poles on the FLA 8000 are engaged with the holes in the carrier 2 6 Close the carrier locking cover firmly Close it so that the poles on both sides of the carrier...

Page 85: ...door 3 Go back to the PC and click on Setting in the PC window on the right of Carrier Set in the upper part of the window or lower left of the window 4 The window for the setting the scanning condit...

Page 86: ...to 100 um Note Gel scanning speed The gel scanning speed is fixed to 400 mm second 1 2 Specifying gel to be scanned and laser filter and high voltage conditions Tick the box below an intended gel icon...

Page 87: ...The filter types available for a laser differ with the laser types The available combi nations are as shown below 635 mm 675 DF 20 Optional filter added 532 mm 570 DF 20 675 DF 20 Optional filters add...

Page 88: ...You may save and load recall the scanning conditions Saving the scanning conditions Select Save Condition in the pull down menu of the File menu A dialog box opens Input an intended name in the dialog...

Page 89: ...indow to start final scanning Scan Mode 1 Gel being scanned is marked with Scanned images are displayed in the window in sequence 2 Image window in the Scan mode There are several methods to display t...

Page 90: ...2002 Jan Verson 3 0 88 Cascade Displays overlaid images Tile Horizontal Displays images horizontally Scanning Gel Samples Scan Mode...

Page 91: ...2002 Jan Verson 3 0 89 Tile Vertical Displays images vertically Arrange Icons Windows illustrated as title bars are arranged Scanning Gel Samples Scan Mode...

Page 92: ...2002 Jan Verson 3 0 90 3 When Catalog is selected on the Window menu the minimized catalog window is enlarged and displayed in the forefront Scanning Gel Samples Scan Mode...

Page 93: ...e Image scanned by the green 532 nm laser overlaid on the image scanned by the blue 473 nm laser Blue red Image scanned by the blue 473 nm laser overlaid on the image scanned by the red 635 nm laser R...

Page 94: ...a displayed image click the button and click the mouse button in a position to be contracted in the image window To move a displayed image without changing the magnification click the button and drag...

Page 95: ...d images select a scale in the pull down menu and adjust the gradation with the adjuster bar on the histogram or by dragging the button below the histogram 5 3 Changing the color of displayed images Y...

Page 96: ...n 3 0 94 6 Click on the Save icon in the Scan mode window or the Save button in the lower left The Scan mode window changes into the Save mode window in which scanned images are saved Scanning Gel Sam...

Page 97: ...iles automatically in a certain rule Serial numbers are added to the end of image file names Each file name consists of the characters shown in the following example in principle xxx 1_473 Click the b...

Page 98: ...d im ages Clicking the button resets the suffices added to the previous data and gives the file name xxx 1 to the first one of newly scanned im ages If you input a character string in the Base Name bo...

Page 99: ...mat Click the button or the File Format tag in the Preference dialog box of the Naming Rule Option described above A dialog box for specifying a file format is displayed You may select one of the foll...

Page 100: ...tting Fixed Images are automatically converted into the TIFF file format using the algorithm in the software based on the maximum value of the images Custom Input a numeric value which indicates the c...

Page 101: ...this box is saved together with the image data 5 Selecting an image to be saved The file will be saved as long as the check box on the left side of the gel icon is chosen Deselecting the check box by...

Page 102: ...2002 Jan Verson 3 0 100...

Page 103: ...2002 Jan Verson 3 0 101 Setting Optional Filters Part 8 Setting Optional Filters...

Page 104: ...ndard filters Set the optional filters in the procedures shown below 1 Click on the Option menu to display the pull down menu Select Filter Change 2 Open the filter adding door on the right side of th...

Page 105: ...tting Optional Filters 4 Remove the filter ring from the filter block using the tool and remove the shield plate 5 Prepare a filter of the following dimensions Diameter 25 mm Thickness 4 mm or more 12...

Page 106: ...2002 Jan Verson 3 0 104 Setting Optional Filters 7 Set the filter block with the filter on the front side 8 Close the filter adding door...

Page 107: ...2002 Jan Verson 3 0 105 Part 9 Daily Maintenance...

Page 108: ...e interval shorter than a month 1 Maintenance of SHG Laser Operation procedures 1 Switch on the FLA 8000 body and analyzer unit 2 Make sure that they have started up Then turn them off CAUTION Activat...

Page 109: ...2002 Jan Verson 3 0 107 Part 10 Troubleshooting...

Page 110: ...ective You may not use the specific scanning mode where the defective laser is used Other scanning modes are available 2 Analog circuits are adjusted improperly You may not use the combination of that...

Page 111: ...ver during the self diagnosis and startup adjustment of the FLA 8000 about fifteen minutes after turning on the power Close the cover CASE 2 Didn t you open the cover during scanning Close the cover i...

Page 112: ...urs in the FLA 8000 body Beep sound Be beep be beep be beep An error message dialog box is displayed on the Image Reader screen displayed on the analyzer unit Refer to Countermeasures according to war...

Page 113: ...f diagnosis first when a trouble occurs in order to solve a trouble smoothly Self diagnosis procedures When an error occurs remove the carrier close the cover and execute a self diagnosis without swit...

Page 114: ...ecuted for about five minutes and a text file having the following file name is created automatically xxxx_DIAG_xxxx 5 The following dialog box is displayed again Click the button 6 Click on Short to...

Page 115: ...r execute self diagnosis Countermeasures according to warning and error messages 7 When an error code is displayed memorize it and click the OK button A text file having the following file name is cre...

Page 116: ...ent upper limit A Peltier error bit is detected A thermistor error bit is detected No lasers are installed Close the sample setting door or filter exchanging door If the phenomenon does not change on...

Page 117: ...interruption is failed while monitoring the inte gral current The integral current could not be adjusted within the target value range A line interruption is failed while monitoring the inte gral volt...

Page 118: ...out error while moving toward the part with pin holes Pinhole unit error Sensor OFF error after moving toward the part with pinholes Pinhole unit error Time out error while moving toward the part wit...

Page 119: ...oving to the origin after receiv ing an origin command Sub scanning unit error Time out error while moving out of the origin Sub scanning unit error Sensor ON error after moving out of the origin Sub...

Page 120: ...rror Sensor ON error after moving a specified filter other than the optional filter Main scanning unit error Time out error while moving after receiving specified pulses or an origin command Sub scann...

Page 121: ...mand Focusing unit error Exceeding the specified positive position Focusing unit error Exceeding the specified negative position Sub scanning unit error Time out error while moving after receiving spe...

Page 122: ...e specified number of times Lamp current check error The monitored lamp current is below the standard PMT sensitivity check error The difference between the data of the equipment and the sensitivity f...

Page 123: ...the user manual and run diagnostics FLA 8000 detected a problem Please see the user manual and run diagnostics FLA 8000 detected a problem Please see the user manual and run diagnostics Moving when fo...

Page 124: ...user manual and run diagnostics FLA 8000 detected a problem Please see the user manual and run diagnostics FLA 8000 detected a problem Please see the user manual and run diagnostics FLA 8000 detected...

Page 125: ...er manual and run diagnostics FLA 8000 detected a problem Please see the user manual and run diagnostics FLA 8000 detected a problem Please see the user manual and run diagnostics FLA 8000 detected a...

Page 126: ...the user manual and run diagnostics FLA 8000 detected a problem Please see the user manual and run diagnostics FLA 8000 detected a problem Please see the user manual and run diagnostics FLA 8000 detec...

Page 127: ...lem Please see the user manual and run diagnostics FLA 8000 detected a problem Please see the user manual and run diagnostics FLA 8000 detected a problem Please see the user manual and run diagnostics...

Page 128: ...nd run diagnostics FLA 8000 detected a problem Please see the user manual and run diagnostics FLA 8000 detected a problem Please see the user manual and run diagnostics FLA 8000 detected a problem Ple...

Page 129: ...ected with an AC outlet properly CASE 2 An abnormal image is displayed A virtually impossible image is displayed An image is displayed in a wrong position Other Troubles 1 Impacts or vibrations are ap...

Page 130: ...2002 Jan Verson 3 0 128...

Page 131: ...2002 Jan Verson 3 0 129 Part 11 Specifications...

Page 132: ...rauschpegelmesser Fuji Kalibrierung Nr 8697 in einer Entfernung von 1 m vom Gerat und einer Hohe von 1 2 m uber dem Boden Major Specifications Image Scanning Specifications Applicable samples Slide gl...

Page 133: ...100 V Approx 7 A 200 V IP eraser 1 25 A 100 V 0 7 A 200 V Pictorography 12 A 100 V 6 A 200 V 5 Grounding Class D grounding This equipment must be grounded by connecting the power cable plug with a 2P...

Page 134: ...2002 Jan Verson 3 0 132...

Page 135: ...2002 Jan Verson 3 0 133 Part 12 Warranty...

Page 136: ...is installed 4 If the user carries out improper operation or maintenance 5 If a trouble originates from moving or transportation after installation 6 If a trouble is caused by irresistible forces suc...

Page 137: ...2002 Jan Verson 3 0 135...

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