93
D.3 Examples of Program Settings with Staining Results
Following are examples of buccal specimens prepared using a smear technique and stained using different
program setting. The sides were cleared and then cover slipped and viewed at a magnification of 10x.
Table 15: Program Settings with Staining Results 1 and 2
Program Setting Figure 1 Figure 2
Prewash Alcohol
1
1
Prewash Bluing
0
0
Nuclear/Htox
1
1
Acid Alcohol Wash
3
9
Bluing
1
1
Bluing Wash
2
2
Orange G
1
1
Orange G Wash
2
2
Cytoplasmic/EA
1
1
Final Wash
2
2
End Spin
0
0
Figure 1
Figure 2
Appendix D: Stain Modes and Programming Options
