88
D.1 Staining Sequence
Table 13: Staining Sequence
NOTE:
Table 13 represents the staining sequence with program settings with an approximate stain cycle
duration in minutes:seconds.
Program Step
Progressive
Regressive
1:1
5:5
9:9
1:1
5:5
9:9
Prewash Alcohol
0
0
0
0
0
0
Prewash Bluing
0
0
0
0
0
0
Nuclear/Htox
1
5
9
1
5
9
Acid Alcohol Wash
0
1
3
1
5
7
Bluing
1
2
3
1
2
3
Bluing Wash
2
3
5
2
4
7
Orange G
1
5
9
1
5
9
Orange G Wash
2
3
5
2
5
8
Cytoplasmic/EA
1
5
9
1
5
9
Final Wash
2
3
6
2
5
8
End Spin
1
1
1
1
1
1
Approximate Stain Cycle Duration (minutes:seconds)
12-Slide Carousel
4:39
8:31
19:30
4:53
9:15
22:38
30-Slide Carousel
7:05
12:30
28.03
7:19
13:35
32.03
Appendix D: Stain Modes and Programming Options
Appendix D: Stain Modes and Programming Options
