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2.2 SETTING UP STEP-BY-STEP
1. Interface – PC Connection:
Data acquisition is possible either by connecting the 620M Interface directly to a PC with Labchart Pro and the
DMT Device enabler installed or through the BNC connectors and BNC cables from the 820M interface e.g. to
the Powerlab box and the Powerlab box is then connected using a USB cable to the computer with Labchart
installed(optional).
I. Direct PC Connection:
Connect the 620M Interface using the applied USB cable directly to the PC with
Labchart Pro installed (version 8 or newer).
II. PowerLab (Optional):
Connect the 820M Interface to the PowerLab unit using BNC cables. Connect Rec 1on
the Interface to Input 1on the Power Lab, Rec 2 to Input 2 etc. Connect the PowerLab unit to one of the USB-
ports on the PC using the USB cable delivered with the PowerLab system.Connect the Powerlab box using
the USB cable to the computer with Labchart installed.
2. Oxygen Supply:
Connect the gas supply (95% O2, 5% CO2 or 21% O2, 5% CO2, balance N2) with tubing running from the gas
supply to the gas inlet on the back of the Interface. Oxygen is supplied to the chambers by tubing attached to the
stainless steel vacuum pipe. The oxygen and vacuum tubing need to be inserted into the chamber in order to
aerate the heated buffer. Needle valves on the back of the interface can be adjusted to regulate the amount of
bubbling that occurs. Turning the regulator clockwise increases the bubbling while turning it counter-clockwise
decreases the bubbling. Each regulator has a lock device attached that can be used when the desired bubbling
is achieved. See Figure 2.2.
This chapter contains a complete step-by-step description of how to set up a complete myograph 820M system as
illustrated in Figure 2.1.
3. Vacuum Connection:
The system has a built-in manifold with separate valves that allows each chamber to be drained individually.
After connecting the vacuum source at the back of the Interface, the vacuum pipes need to be inserted into the
chambers in order for this feature to work properly. The pipes are inserted into the chamber by gently pulling
NOTE: The needle valves need to be greased (using the grease for the linear slides – the brown
grease) and turned at regular intervals to prevent them from sticking or permanently freezing.
