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CHAPTER 5 - THE MOUNTING SUPPORTS AND
MOUNTING IN THE 820MS CHAMBER

Each chamber contains supports with mounting clamps to facilitate the mounting of muscle strips in the myograph
chambers. These clamps have been designed to optimally “clamp” the tissue without slipping during a contraction.
They are also designed to effectively “grab” tendon on whole striated muscle preparations without experiencing
“slippage” during a whole striated muscle contraction.

1. Starting position.

2. Loosen the screws on the mounting clamps.

3. Turn the upper wings of the mounting

clamps to expose the base of the support.

4. Place the muscle strip onto the base of the

mounting clamp.

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Summary of Contents for 820MS

Page 1: ...USER MANUAL VOL 3 2 M Y O G R A P H S Y S T E M 8 2 0 M S ...

Page 2: ......

Page 3: ...the time of printing Changes may have been made to the software and hardware it describes since then New information may be supplied separately This documentation is provided with the DMT Muscle Strip Myograph System Model 820MS Document Number 820MS UG2 2A All rights reserved No part of this manual may be reproduced or transmitted in any form or by any means without the written permission of Dani...

Page 4: ...h System 820MS 11 2 2 Setting up step by step 12 CHAPTER 3 THE INTERFACE MENUS 14 3 1 Interface Menus Navigation 14 3 2 Interface Menus Calibrated Mode 15 CHAPTER 4 THE MUSCLE STRIP MYOGRAPH UNIT 27 4 1 Calibration of the Force Transducer 27 4 2 Checking the Force Transducer 29 4 3 Force Transducer Replacement 30 4 4 Myograph Maintenance 32 4 5 Cleaning the Myograph 33 CHAPTER 5 THE MOUNTING SUPPO...

Page 5: ...ttachments and accessories specified by the manufacturer Unplug this apparatus during lightning storms or when unused for long periods of time Be advised that different operating voltages require the use of different types of line cord and attachment plugs Check the voltage in your area and use the correct type See the table below SAFETY Protect the power cord from being walked on or pinched parti...

Page 6: ...em In case of transport and the original packing material is unavailable please contact DMT Sales Department for advice and packing instructions After unpacking your new Myograph System please use the following list to check that the system is complete 1 x interface unit 4 x chamber units with mounted clamp supports 4 x chamber covers 1 x external temperature probe 1 x power cord 1 x calibration k...

Page 7: ...ts of the EMC Directive 2004 108 EC Based on the following specifications applied by EN 61326 1 2006 EN 61326 2 6 2006 EN 61326 2 6 Corr 2007 And with the LVD Directive 2006 95 EC Based on the following specifications applied by EN 61010 1 2010 EN 61010 2 030 2010 General warnings regarding EMC Do not use this device in close proximity to sources of strong electromagnetic radiation e g unshielded ...

Page 8: ...8 CHAPTER 1 SYSTEM OVERVIEW Figure 1 1 Interface Front Panel Myo Interface display Valve buttons Heat indicator Power indicator 1 1 INTERFACE ...

Page 9: ...EAR PANEL Gas regulator needle valve Gas input 4 Transducer connections pH meter connection Temperature probe Connection 4 Recorder outputs Analogue USB output Digital ON OFF switch Power connector Vacuum input Grounding connected to Data Acquisition ...

Page 10: ...raph clamp support connected to micrometer Myograph clamp support connected to force transducer Force transducer pin Figure 1 4 Close up of myograph unit 1 3 MYODYNAMICS MUSCLE STRIP MYOGRAPH 840MD Figure 1 3 MyoDynamics Muscle Strip Myograph unit ...

Page 11: ...em 820MS as illustrated in figure 2 1 above Figure 2 1 The complete Muscle Strip Myograph System 820MS DMT CS4 CS8 Stimulator optional PC data acquisition and analysis software optional Myo Interface front panel PowerLab data acquisition system optional BNC Cables Connection to oxygen supply Vacuum pump optional Suction bottle optional Myo Interface rear panel USB DMT Device enabler option ...

Page 12: ... gas inlet on the back of the Interface Oxygen is supplied to the chambers by tubing attached to the stainless steel vacuum pipe The oxygen and vacuum tubing need to be inserted into the chamber in order to aerate the heated buffer Needle valves on the back of the interface can be adjusted to regulate the amount of bubbling that occurs Turning the regulator clockwise increases the bubbling while t...

Page 13: ...rms and gas vacuum tubes Figure 2 3A Chamber cover Figure 2 4B Chamber cover with built in electrodes optional item Prior to the shipment of the Multi Wire Myograph 820M System has gone through two days of continuous testing including a final force transducer calibration However DMT recommends that a new force transducer calibration is performed before using the myograph system for the first time ...

Page 14: ...re detail in this chapter Menus on the 820MS interface are all accessible by a touch screen To access a menu simply touch the screen A setting can be changed by touching the SELECT icon on the screen corresponding the SELECT icon on the touch screen corresponding to the desired channel to be changed SELECT SELECT SELECT SELECT ENTER The line to be modified will turn blue indicating that the interf...

Page 15: ...put from the BNC connectors is 2 5 volts POWER UP SCREEN After turning on the 820MS Interface an Introduction screen appears The system is auto calibrating the A D converters while this screen is displayed 3 2 INTERFACE MENUS CALIBRATED MODE After a few seconds the ACTUAL FORCE display will appear At any given time if the force applied on any channel is out of range the force reading for the overl...

Page 16: ...CT or all at once by pressing ALL Pressing ENTER will execute the zero function HEAT MENU The heating unit and temperature are controlled from this menu To turn the heat on or change the preset temperature for the system access the temperature control menu Pressing the HEAT key will enter the menu and allow the user to change the default system temperature as well as turn the heat on or off Pressi...

Page 17: ...INTERFACE SETTINGS These are the options that are present when the system is in Calibrated Mode Different options will appear when the system is in Direct Mode 1 Force calibration NOTE everytime a force calibration is performed the MEASUREMENT RANGE is set to default 200 mN Set measurement range after the force calibration Touch SELECT to initiate the calibration for the chosen chamber and the tex...

Page 18: ... clamp support or mounting pin without touching in order to get the most accurate calibration Press NEXT STEP when the calibration kit has been properly placed Step 3 Initiates the heating process for the chambers In order to perform an accurate calibration the transducers must be heated to the desired experimental temperature to accommodate heat induced expansion of the electronic parts in the tr...

Page 19: ...N of force The weight placement should cause a positive increase in the 4 digit number Wait at least 30 to 40 seconds for the applied force to stabilize before pressing NEXT STEP Once the 4 digit number has stabilized press NEXT STEP Step 6 is to verify that the calibration was performed correctly The Force Chamber 1 reading should be 9 81 0 1 mN If the Force Chamber 1 reading is off by more than ...

Page 20: ...ress NEXT STEP NOTE Before the pH calibration is performed be sure to select the way the pH electrode is to be used See the sub menu pH Set up under Interface Settings Step 2 Place the pH electrode and temperature probe in the high buffer solution here pH 7 and turn on stirring of the high buffer solution When the relative pH output in the blue line is stable go to NEXT STEP Step 3 Place the pH el...

Page 21: ...priate setting so as to capture any maximal response from the vessel of interest This value should not exceed the settings for the transducer range which is defined by the sub menu MEASUREMENT RANGE and is explained in the next section The SELECT and ALL functions are the same in this menu as previously described for the VALVE DELAY menu Pressing ENTER will store the numbers in memory for future e...

Page 22: ...LVE DELAY menu Pressing ENTER will store the numbers in memory for future experiments 5 Interface settings The INTERFACE SETTINGS sub menu in SETTINGS has an additional 6 sub menus These 6 additional sub menus are I Temperature Difference II Valve Delay III pH Set up IV Select mN or g V Factory Diagnostics VI Myograph Type I TEMPERATURE DIFFERENCE The TEMPERATURE DIFFERENCE function allows the use...

Page 23: ... enough time to completely empty a chamber with even as small a volume of 5 ml Pressing ALL will cause all the lines to turn blue meaning all chambers can be modified at the same time Again the up and down arrow keys can be used to modify the length of time the vacuum valves stay open Pressing SELECT next to any given channel will cause the line selected to turn blue The up and down arrow keys can...

Page 24: ...mp Value 20 C pH SET UP MENU SELECT SELECT SELECT Low buffer pH 4 0 High buffer pH 7 0 Temperature comp MANUAL ENTER SELECT Manual temp Value 20 C SELECT Force in mN ENTER SELECT mN OR GRAM SELECT Force in mN ENTER SELECT mN OR GRAM IV Select mN or g The contraction force can be shown in gram or in mN on the touch screen and for Labchart Pro and DMT device enabler users the selected force unit wil...

Page 25: ...d for diagnostics and troubleshooting purposes The general user will not have access to this window Entering the proper 5 digit pin number however will allow the trained technician access to Diagnostics panels that will provide information during a malfunction or mechanisms to change other settings controlled by the onboard computer VI Myograph Type The interface of the 820MS system is identical t...

Page 26: ...etters indication that this systems menu structure is selected Turn off the power of the system and turn it ON again Now the system will load the menu s of the select myograph system into the interface and is now ready for use It is important to make a new weight calibration and make sure that the Measurement Range is correct If the device enabler is not used the then also check the Force Rec Outp...

Page 27: ...ep procedure is included in the FORCE CALIBRATION sub menu under SETTINGS as explained in Chapter 3 The Interface Menus on page 17 FORCE TRANSDUCER CALIBRATION PROCEDURE This section contains step by step instructions to calibrate the force transducer NOTE Every time a force calibration is performed the MEASUREMENT RANGE is set to default 200 mN Set measurement range after the force calibration CA...

Page 28: ...the bridge with the pans is not touching any part of the mounting support Go to the main menu displaying the forces and zero the channel being calibrated so the force reads zero Slowly and gently slide the calibration kit forward toward the micromanipulator so that the transducer arm rests on the mounting support creating a force reading on that channel Carefully slide the calibration kit back tow...

Page 29: ...l unstable or noisy then perform a new calibration of the force transducer as described in Chapter 3 The Interface Menus on page 17 and 4 1 Calibration of the force transducer on page 27 During the new calibration monitor the relative force reading values in the FORCE CALIBRATION sub menu on the Interface Steps 4 and 5 of the calibration procedure If the value is 0 a single digit or above 6500 the...

Page 30: ...r house 5 Place a VERY small amount of vacuum grease clear or whitish grease around the bottom inside of the transducer housing to seal the transducer housing when put back in place An arrow in figure 4 5 indicate the place that the grease needs to be applied 6 Carefully realign the transducer housing with the new transducer on the Myograph Chamber and reinsert the allen screws through the bottom ...

Page 31: ...igure 4 5 The transducer in the transducer housing and close up of transducer pin inside the transducer The arrow indicate the place that the grease needs to be applied to prevent water and buffer from damaging the transducer Figure 4 6 Close up of transducer pin from outside The arrow indicate the place that the grease needs to be applied to prevent water and buffer from damaging the transducer ...

Page 32: ...emove any water or buffer remaining in the chamber or on the tubing using absorbent paper FORCE TRANSDUCER The force transducer is the most delicate and fragile component of the myograph system Extreme care must be used when handling or touching the force transducers As a part of daily maintenance inspect the grease around the transducer pin extending from the transducer housing pinhole before sta...

Page 33: ...ned after each experiment At the end of each experiment use the following procedure to clean the myograph chambers and supports 1 Fill the myograph chamber to the edge with an 8 acetic acid solution and allow it to work for a few minutes to dissolve calcium deposits and other salt build up Use a cotton tipped applicator to mechanically clean all chamber surfaces 2 Remove the acetic acid and wash t...

Page 34: ...UFFER AND WATER FROM ENTERING THE TRANSDUCER HOUSING If red or brown discolorations appear on the chamber sides or on the supports the following cleaning procedure will work in most cases 1 Incubate the myograph chamber and supports for 2 minutes with 2mM T 1210 Tetrakis 2 pyridylmethyl ethylenediamine solution dissolved in double distilled water 2 Use a cotton tip applicator to mechanically clean...

Page 35: ...AMP MAY NEED TO BE REMOVED FROM THE MYOGRAPH CHAMBER AND CLEANED INDIVIDUALLY TO ASSURE PROPER CLEANING OF ALL SUPPORT SURFACES NEVER SOAK THE SUPPORTS IN ANYTHING STRONGER THAN 8 ACETIC ACID FOR EXTENDED PERIODS OF TIME I E SEVERAL HOURS OR OVERNIGHT ...

Page 36: ...ptimally clamp the tissue without slipping during a contraction They are also designed to effectively grab tendon on whole striated muscle preparations without experiencing slippage during a whole striated muscle contraction 1 Starting position 2 Loosen the screws on the mounting clamps 3 Turn the upper wings of the mounting clamps to expose the base of the support 4 Place the muscle strip onto th...

Page 37: ...scle strip is clamped between the wings and base of the mounting clamp 6 Secure the screws IMPORTANT BE VERY CAREFUL WHEN TIGHTENING THE SCREW ON THE TRANSDUCER SIDE EXCESSIVE PRESSING DOWN ON THE SCREW OR EXCESSIVE TORQUE FORCE WILL DAMAGE THE FORCE TRANSDUCER ...

Page 38: ...dually controlled per chamber by needle valved Force range User selectable at 200 400 800 1600mN Force resolution 0 1 mN Weight calibration Semi automatic Heating Built into chamber independent of super fusion Temperature range Ambient temperature 50 C Temperature resolution 0 1 C Temperature probe External Output reading Force mN or Grams g Analogue output Independently filtered or unfiltered dir...

Page 39: ...486 9685 Fax 86 21 5877 0063 DMT Asia Pacific Tel 61 2 8814 1597 Danish Myo Technology A S E mail sales dmt dk Tel 45 87 41 11 00 Fax 45 87 41 11 01 DMT USA Inc E mail sales dmt usa com Tel 1 734 707 0250 Fax 1 678 302 7013 ...

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