diagenode Bioruptor Pico, User Manual

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6 BIORUPTOR
®
PICO
MANUAL
Critical steps for maintenance
and efficient shearing
General warnings
Sonication bath temperature
• Optimal temperature for sonication is 4°C. Sample temperature should not exceed 8°C.
• The Bioruptor Cooler (Cat. No. B02010010, B02010011, B02010012) has to be used to guarantee the
automatic temperature control of the sonication bath during the entire sonication process.
Validated tubes for the Bioruptor
®
Pico
• DNA shearing: 0.2 ml (Cat.No. C30010020) and 0.65 ml (New Cat. No. C30010011; Old Cat. No. WA-005-
0500) Bioruptor
®
Microtubes for DNA shearing.
• Chromatin shearing: 0.2 ml (Cat.No. C30010020) and 1.5 ml (Cat.No. C30010016) Bioruptor
®
Microtubes
and 15 ml (Cat.No. C01020031) Bioruptor
®
Tubes & sonication beads.
Fitting tubes in the tube holder
• Place the tubes in the corresponding tube holder. Never leave empty spaces in the tube holder. Fill the
empty spaces with tubes containing the same volume of water. Screw the lid on the tube holder without
over-tightening it.
• Carefully place the tube holder on the holding plate.
• During sonication, samples must remain at the bottom of the tube. If needed, briefly centrifuge samples
during sonication after pausing the run.
DON’T
Turn on the instrument without water
Exceed 30 min of total sonication
Tilt the sonication unit
DO
Change water at least once every month
Use deionized or distilled water
QC your system with our DNA QC Kit