Standard Curve Methods: Fluorescence
New Standard Curve Assays/Methods
Each new method requires a unique name. In addition, the following parameters
must be defined:
Excitation Source
• Use
LED Radio
buttons to select the excitation source.
• The
LED Wizard
may be used to determine the optimal excitation source if
not known. The software will make a measurement using each of the three
excitation sources and will report the associated RFU in the color-coded
boxes.
• Some fluorophores do not exhibit significant fluorescence unless bound to
a protein or nucleic acid. It may be necessary to set up an assay reaction
to best use the LED Wizard.
• The standard curve assay methods use one excitation source to determine
the concentration of a sample based upon the RFU measured using the
corresponding emission filter set.
Advanced Options
• Use the
Advanced Option
buttons to set both the Trend Line/Blank
selections as well as the standard curve units.
Although all accounts may use any saved method, only the method account
holder or an administrator may modify or delete the new method.
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Summary of Contents for DS-11 FX+
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