DeNovix DS-11 FX+ User Manual Download Page 55

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14. Fluorescence: Standard Curve Method Apps

The five apps listed below are designed using the same basic architecture. Each
app (except the Custom Std Curves app) includes preconfigured assays
designed to accommodate commonly used commercially-available reagent kits.

Fluoro

dsDNA

•

DeNovix dsDNA Broad Range

•

DeNovix dsDNA High Sensitivity

•

DeNovix dsDNA Ultra High Sensitivity

•

Quant-iT

™

PicoGreen

•

Quant-iT

™

dsDNA HS

•

Quant-iT

™

dsDNA BR

•

Qubit

™

dsDNA BR

•

Qubit

™

dsDNA HS

•

Quantifluor dsDNA

•

Quantifluor One

dsDNA

Fluoro

RNA

•

Quant-iT

™

RiboGreen

•

Quant-iT

™

RNA HS

•

Quant-iT

™

RNA BR

•

Qubit

™

RNA HS

•

Qubit

™

RNA BR

•

Quantifluor RNA

•

Quant-iT

™

microRNA

•

Qubit

™

microRNA

Fluoro

ssDNA

•

Quant-iT

™

OliGreen

•

Qubit

™

ssDNA

•

Quantifluor ssDNA

Fluoro

Protein

•

Quant-iT

™

Protein

•

Qubit

™

Protein

Fluoro

Std Assays

•

All entries to be defined by the user

Summary of Contents for DS-11 FX+

Page 1: ...Rev December 2018 ...

Page 2: ...Basic Operation 15 Typical Absorbance Run Screen 17 Best Practices Absorbance Measurements 19 6 Absorbance Nucleic Acid Apps 20 dsDNA RNA and ssDNA 21 Microarray 22 7 Absorbance Purified Protein Peptide Apps 28 Protein A280 29 Labeled Proteins 31 Peptides 33 8 Absorbance Colorimetrics App 36 Generating a Standard Curve 37 BCA 40 Bradford 40 Lowry 40 Pierce 660 40 9 Absorbance UV Vis App 41 10 Abso...

Page 3: ...55 15 Basic Fluorometer App 62 Excitation Selection and Emission Results 63 16 Data Export and Print Options 64 Export Options 64 Export Via Email 65 Export to a Network Folder 66 USB 68 Print Options 70 17 Utility Apps and Functions 72 Accounts App 72 Data App 74 Export vs Archive 75 Settings App 77 Updater App 80 18 Lab Tool Apps 81 Calculator App 81 Timer App 82 19 Maintenance 83 Cleaning 83 So...

Page 4: ...agnostics 85 DS 11 Self Test 85 Microvolume Pathlength Verification 86 Verification Check Troubleshooting 87 FX Diagnostics 89 21 Troubleshooting 90 Quick Help 90 Software Errors 92 22 Customer Support 93 4 ...

Page 5: ...ically adjusts the microvolume mode pathlengths to enable a sample measurements across a broad concentration range Absorbance range specifications can be found at www denovix com FX models include a fluorescence mode while the DS 11 and DS 11 FX instruments also enable cuvette based measurements for low concentration samples and basic absorbance kinetic studies Key Features include Integrated Proc...

Page 6: ...operating system will boot up in less than a minute 4 Use the Shut Down icon accessed from within each app or on the Home screen to do a soft power down Cautions NE PAS OUVRIR L APPAREIL Aucun composant réparable par un utilisateur est inclus Confiez l entretien à du personnel qualifié DO NOT REMOVE COVER No operator serviceable components inside Refer servicing to qualified personnel The DS 11 is...

Page 7: ...near a radio or television receiver in a domestic environment it may cause radio interference Install and use the equipment according to the instruction manual This equipment has been tested for radio frequency emissions and has been verified to meet CISPR 22 2 Class B Instrument Rear Panel The DS 11 comes equipped with one Ethernet and three USB ports The Ethernet feature is provided for internet...

Page 8: ... disappeared before performing additional actions The USB Flash Drive Eject icon appears in the bottom notification bar when a drive is detected The software will automatically safely unmount the flash drive after a screen capture or a report is exported to the drive Tap the icon to remove a flash drive when nothing was exported or saved The Quick Print icon is only displayed on Report or Graph sc...

Page 9: ...andards curve enables one or up to three replicates The fluorescence apps Basic Measure button is used to make single measurements not being read from a standard curve Fields with Drop Down Menus are indicated by black or white triangles located on the center bottom Fields with black underlines are editable by the user Tapping on the field will enable a keyboard a numeric keypad or a dialog box Ap...

Page 10: ...op bar or by swiping the screens left or right Use the Current Account Tab drop down menu on the top action bar in the Run Report and Graph screens to select a specific account within an app Use the Accounts app to create and manage accounts The bottom bar Back button is used to navigate to the main level of the current app The bottom bar Home button is used to navigate to the Home screen without ...

Page 11: ...tly deletes it from the instrument Clear Samples This function saves and then clears all current sample records from the Report and Graph screens Baseline Correction Some apps enable Baseline Correction changes from the Overflow function on the Run screen User Guide The User Guide may be accessed either from the Overflow icon within any app or by launching the User Guide app About The About featur...

Page 12: ...ries significantly between the absorbance and the fluorescence apps Refer to the Basic Operation section for the absorbance mode and the individual fluorescent app sections for more information Report Screen The Report screen list includes application specific data fields for each sample measured in the current measurement session Tap rows to highlight and select data for export The Select All UnS...

Page 13: ...d sample data may be exported or printed using the top action bar Overflow Screen Capture or Export Selected functions Additional information is provided in the chapter entitled Screen Capture and Export Graph Screen Tap rows to highlight and select data to display the spectra The Select All UnSelect All icons are used to quickly select or deselect all rows Up to 24 samples may be overlaid on the ...

Page 14: ...ta app Clear highlighted sample records from the both the Report and Graph screens using the Delete icon The cleared data will be available using the Data app Highlighted sample data may be exported or printed using the top action bar Overflow Screen Capture or Export Selected functions The data will will exported as a csv file and spectra may be re graphed using a spreadsheet chart function Addit...

Page 15: ...re button All microvolume measurements are reported in 10 mm equivalent absorbance values Cuvette Measurements Refer to the light path arrow as a guide when inserting a cuvette Use cuvettes that meet the following specifications Width 12 5 mm Length 12 5 mm Height 45 mm Z height 8 5 mm UV wavelength region Use quartz or UV transparent plastic cuvettes Visible wavelength region Use quartz or plasti...

Page 16: ...volume mode uses 1 0 μL samples pipetted onto the sample surface The microvolume Short Path mode requires 0 5 μL samples pipetted onto the sample surface This mode is for high concentration samples and does not use the 0 5 mm pathlength The microvolume Fast Mode option is primarily for samples in the range measured by the 0 5 mm pathlength although it may be used for higher concentration samples T...

Page 17: ...601 USB scanner to enter sample names Sample Type and Factor Fields Displays current sample type and associated factors used for concentration calculations The fields may be fixed or selectable depending on the application Absorbance Graph The spectral plot displays data for the current sample normalized to a 10 mm pathlength for microvolume measurements Pinch and zoom anywhere in the graph to res...

Page 18: ...in the background prior to turning off the instrument Mechanism Busy Indicator A progress bar will be displayed on the screen when the measurement mechanism is busy Please do not lift the arm push software buttons or turn the instrument off while a progress bar or other busy indicator is displayed Measurement Sessions New measurement sessions begin each time an app is accessed from a closed state ...

Page 19: ...ach sample aliquot Avoid introducing bubbles when pipetting samples onto the measurement surfaces Ensure sample concentrations fall within absorbance limits of the instrument for accurate results Use the same buffer a sample is suspended in for the Blank measurements Avoid using buffers such as RIPA buffers that contain components with strong absorbances at the wavelength of interest Measure a fre...

Page 20: ...ple type if applicable Enter optional user defined ng cm μL factor ssDNA and Microarray Select a dye type Microarray only 5 Measure a fresh aliquot of sample using the Measure button Notes Blank with TE if the sample is dissolved in TE Ensure that the sample isolation procedure is optimized and that samples are purified prior to making absorbance measurements Ensure all solutions are homogenous an...

Page 21: ...μL RNA 40 ng cm μL ssDNA 33 ng cm μL Oligo factors derived from the Calculator app may be copied and pasted directly into this field Concentration Results Concentration results are reported in units of ng μL Units may be changed using the Run screen top action bar Overflow menu The units selected at the time of the measurement is what is saved to the database A260 Reports the baseline corrected 26...

Page 22: ...alculator app may be copied and pasted directly into this field Dye Type Cy3 is default selection for this application Nucleic Acid Concentration Results Results are reported in units of ng μL Units may be changed using the Run screen top action bar Overflow menu The units selected at the time of the measurement is what is saved to the database Dye Fluorophore Concentration Results Results are rep...

Page 23: ... for both the Microarray and Labeled Proteins apps The new dyes may be used by all accounts including the General Account The user added dyes may only be edited or deleted by the account holder or an administrator account holder Dye Ext Coeff A260 Factor A280 Factor Cy3 150000 0 08 0 08 Cy5 250000 0 05 0 05 Cy5 5 250000 0 05 0 18 Alexa Fluor 405 34 000 0 23 0 7 Alexa Fluor 488 71 000 0 3 0 11 Alex...

Page 24: ...for the A260 value and uses a sloping baseline between 400 and 750 nm for dye concentration calculations If adding a new dye it is important to empirically determine if the default 750 nm selection is appropriate to use as the baseline correction wavelength The baseline correction screen is accessed from the Run screen top action bar Overflow icon Tap the Add icon to include a user defined option ...

Page 25: ...the sample spectrum may be offset from the baseline resulting in a change in the reported sample concentration The DS 11 uses a correction at 340 nm as the default selection However the user may elect to define the baseline nm used for specific measurements The baseline correction screen is accessed from the Run screen top action bar Overflow icon Once a baseline correction nm is selected it is ap...

Page 26: ... surfaces measure a new Blank using fresh source of dH20 or sample buffer Use the same solution water or buffer the sample is in for the blank measurement Low 260 280 Dirty measurement surfaces or improper Blank Clean the surfaces measure a new Blank using fresh source of dH20 or sample buffer Use the same solution water or buffer the sample is in for the blank measurement Presence of residual ext...

Page 27: ...ows Users may elect not to have the alerts show or set custom limits using the Run screen Overflow menu The new limits may be used by all accounts including the General Account Saved user added limits may only be edited or deleted by the account holder or an administrator account holder Note The limits are only enforced for samples above a minimum concentration value A blue warning icon will be di...

Page 28: ...be added to the selection list Select a dye type when using the Labeled Proteins app 5 Measure a fresh aliquot of sample using the Measure button Notes If the buffer exhibits significant absorbance at 280 nm use alternative methods such as colorimetric assays to quantitate proteins Ensure all solutions are homogenous and well mixed prior to sampling Use fresh aliquots for each microvolume measurem...

Page 29: ...nits may be changed using the Run screen top action bar Overflow menu The units selected at the time of the measurement is what is saved to the database A280 Reports the baseline corrected 280 nm absorbance value 260 280 Displays the ratio of the 260 and 280 nm absorbance values Protein Type List New Protein types using either user defined E1 or MW and Ext Coeff values may be saved using the List ...

Page 30: ...e the baseline nm used for specific measurements However the user may elect to define the baseline nm used for specific measurements The baseline correction screen is accessed from the Run screen top action bar Overflow icon Once a baseline correction nm is selected it is applied to all subsequent measurements within the same measurement session 30 Sample Type E1 at 280 nm for 10 mm pathlength BSA...

Page 31: ...bar Overflow menu The units selected at the time of the measurement is what is saved to the database Dye Fluorophore Concentration Results Results are reported in units of μM A280 Reports the baseline and dye corrected 280 nm absorbance value This value may be lower than the A280 value observed in the absorbance spectra due to the correction factors applied AXXX The baseline corrected dye specific...

Page 32: ...ors the visual spectrum display to the baseline at 750 nm for all measurements It automatically applies a 340 nm bichromatic normalization for the A280 value and uses a sloping baseline between 400 and 750 nm for dye concentration calculations If adding a new dye it is important to empirically determine if the default 750 nm selection is appropriate to use as the baseline correction wavelength The...

Page 33: ...ength Selector The radio button selector will appear for all sample types except the preconfigured e215 nm and e205 nm options Note Extinction coefficients are wavelength dependent Concentration Results Results are reported in units of mg mL Units may be changed using the Run screen Overflow menu The units selected at the time of the measurement is what is saved to the database AXXX Reports the ba...

Page 34: ...ng equation is used to convert g 100 mL to mg mL concentrations c mg mL A E1 10 The relationship between percent extinction coefficient E1 and molar extinction coefficient Ext Coeff is E1 Ext Coeff 10 molecular weight of protein Daltons Note The Peptide app uses E 0 1 values whereas the Protein A280 app uses E 1 values The DeNovix software reports concentrations in mg mL units for the Protein A280...

Page 35: ... below 0 57 The default software value that will trigger an alert is 0 7 Users may elect not to have the alerts show or set custom limits using the Run screen Overflow menu The new limits may be used by all accounts including the General Account Saved user added limits may only be edited or deleted by the account holder or an administrator account holder Note The limit is only enforced for samples...

Page 36: ...ing at least one replicate for a minimum of 2 standard concentrations Alternatively apply a previously saved curve selected from the drop down menu 6 Navigate to the Run screen Enter a sample name Optional Enter a dilution factor to be applied to the reported concentration 7 Quantitate the sample using the Measure button Notes The Graph Flip button is available on the Run and Graph Screens in the ...

Page 37: ...0 00 concentration may be edited 3 Measure at least one replicate of each standard concentration by highlighting the standard concentration row and then tapping the Measure button Up to 5 replicates may be measured for each concentration Use fresh aliquots for each measurement when using the microvolume mode The software will advance after each measurement until the fifth replicate has been measur...

Page 38: ...concentrations Options include Linear 2nd Order Interpolation This option draws a straight line between each point Note The software will extrapolate concentrations for samples with absorbances 15 above the absorbance values associated with the highest standard for linear fits only Saving a Standard Curve A standard curve or a series of standard concentrations may be saved and reused at a later da...

Page 39: ... name prior to making any changes if you do not want to modify the original curve The only way to recall absorbance data for a standard curve is to save the results as a saved curve before exiting the app Although all accounts may use any previously saved standard curve or saved concentration list only the curve account holder or an administrator may modify or delete a saved curve or concentration...

Page 40: ...dford formulations may form particulates during prolonged incubations It is recommended that the cuvette option be used when using these types of formulations The particulates may interfere with the shorter pathlengths used for the microvolume measurements Lowry The modified Lowry assay is used to quantify proteins based upon the absorbance of tetradentate copper protein complexes The reaction pro...

Page 41: ...n from the Home screen 2 Establish a Blank using the appropriate buffer 3 Enter the analysis nm in the first input field This wavelength is used to determine which pathlength is utilized by the SmartPath technology for microvolume measurements Enter up to five additional wavelengths of interest 4 Enter a sample name 5 Measure a fresh aliquot of sample using the Measure button Wavelength List A lis...

Page 42: ...find the absorbance value of any wavelength after a measurement is made Note The cursor wavelength absorbance value is a temporary value not saved by the software Baseline Correction The baseline correction normalizes the entire spectrum using the absorbance value of the wavelength specified and corrects for baseline offsets due to sample particulates The default selection is set to 750 nm for the...

Page 43: ...g the appropriate buffer 3 Enter a sample name 4 Enter a cell conversion factor optional 5 Measure a fresh aliquot of sample using the Measure button Notes All cell type specific conversion factors are user derived Ensure culture is well mixed prior to sampling Use fresh 1 uL aliquots for each microvolume measurement Although microvolume OD 600 measurements can be made the cuvette 10 mm mode is re...

Page 44: ...ing the absorbance value by the factor Cell numbers are reported in terms of 1 108 cells The factor must be entered into the appropriate field prior to a measurement Note The cuvette mode selection i e the path length must be taken into account when determining target OD 600 values or entering cell conversion factors When making microvolume measurements the OD 600 values reported and used for cell...

Page 45: ...fine additional new methods or edit previously saved methods 3 Navigate to the Run screen and tap the Heater button to preheat the cuvette block to the selected temperature 4 When the Heater temperature field displays the set temperature insert the cuvette and establish a Blank using the appropriate buffer 5 Insert the cuvette with the sample solution and tap the Measure button Notes Use cuvettes ...

Page 46: ...750 nm is the general recommendation but the optimal wavelength should be empirically determined Heater Set Point The heater may be set to temperatures between 37ºC to 45ºC Stage Selections Up to three stages of timed intervals may be included for each method The parameters for each stage include Delay This is the time before the first measurement of each stage Interval This is the time between me...

Page 47: ...ºC of the set point Timer This feature is used to monitor the sample solution warm up time The timer is used in a count up only mode but is not used to track the measurement time Stop Button A Stop button replaces the Measure button on the Run screen during the Kinetics run Tapping this button will end the measurement and save all data accumulated up to that point The Measure button will be availa...

Page 48: ...ort screen list are associated with one assay run Each time a new assay run is initiated the previous results will be saved and then cleared from the Report Graph and Trend screens The cleared data may be accessed using the Data app Note It is normal for the software to sometimes experience minor delays in reporting kinetics data information relative to the actual measurement time point The actual...

Page 49: ... Use the List Add Edit button to the right of the drop down to define additional new methods or edit previously saved methods All methods are available to all accounts However only the account holder or an administrator may edit or delete a method 3 Establish a Blank using the appropriate buffer If the method is a standard curve type establish the Blank and measure the standards from the Std Curve...

Page 50: ...rrection is not used the sample spectrum may be offset from the baseline Although the general recommendation would be to use 340 nm for UV only wavelength ranges and 750 nm for full range methods the optimal baseline correction wavelength should be empirically determined Wavelength Range The software will perform a wavelength optimization function each time the wavelength range is defined or edite...

Page 51: ...custom formula Custom Formulas Tap on the custom formulas input field to display a pop up dialog The following functions are available Advanced When checked this feature allows the user to designate that the method uses a Savitzky Golay filter instead of the default boxcar method to smooth data 51 Function Description Numerical Operations add subtract multiply divide A x Absorbance at wavelength x...

Page 52: ...l sign before the equation This is implied within the software Example A 260 A 280 is correct while A 260 A 280 is incorrect The software automatically applies the baseline correction specified in the method set up Do not include baseline corrections in user defined equations 10 mm pathlength equivalent absorbance values are used and reported for microvolume measurements Cuvette IQOQ mode The cuve...

Page 53: ...Module USB accessory is not detected The table below lists the four LEDs included in all FX models Fluorescence Measurements 1 If using the FX Module accessory connect the module to the DS 11 DS 11 using one of the three back USB ports 2 Insert a micro tube pre filled with the fluorescent solution Close the cover and tap Measure Notes Use clear thin walled 0 5 mL plastic PCR tubes only Measure 200...

Page 54: ...cturers recommendations regarding temperature incubation time and protection from ambient light Follow assay reagent manufacturers recommendations regarding suggested standard curve concentrations and data analysis Ensure all samples and standards are treated identically in terms of incubation times and temperature Minimize assay tube and solution temperature fluctuations as these may impact the a...

Page 55: ... Range DeNovix dsDNA High Sensitivity DeNovix dsDNA Ultra High Sensitivity Quant iT PicoGreen Quant iT dsDNA HS Quant iT dsDNA BR Qubit dsDNA BR Qubit dsDNA HS Quantifluor dsDNA Quantifluor One dsDNA Fluoro RNA Quant iT RiboGreen Quant iT RNA HS Quant iT RNA BR Qubit RNA HS Qubit RNA BR Quantifluor RNA Quant iT microRNA Qubit microRNA Fluoro ssDNA Quant iT OliGreen Qubit ssDNA Quantifluor ssDNA Fl...

Page 56: ...n walled PCR tube into the sample holder and lower the cover Measure at least one replicate for a minimum of 2 standard concentrations 5 Tap the Samples radio button Enter a sample name Optional Enter a dilution factor to be applied to the reported concentration 6 Quantitate a sample using the Measure button Refer to the manufacturer of the assay reagent for assay specific instructions Note DeNovi...

Page 57: ...left panel Preconfigured entries are optimized in regards to excitation source assay units and trend line analysis according to the reagent kit manufacturer s recommendations New assays may be entered using the List Add Edit button User defined assays may be used by all accounts but may be modified or deleted only by the account owner 57 ...

Page 58: ...e Blank is not selected the initial concentration for preconfigured assays will be set to 0 00 This standard is typically the reagent fluorophore solution without any nucleic acid or protein Trend Line If selected the trend line will be forced through the lowest standard concentration When a Blank is used the trend line will be forced through the graph origin 0 0 Use Previous Curve or Std Values C...

Page 59: ...er concentration values or concentration values with the associated RFU values may be saved The New Curve button is used to generate a new curve or load a previously saved curve All sample data generated using the Samples radio button will be automatically saved and accessible using the Data app The Replicates arrow button enables the use of up to three replicate measurements per standard concentr...

Page 60: ... directly from the standard curve trend line Results are reported in terms of the units used to derive the trend line The Optional Dilution Factor or the µl Added value for Qubit assays will be applied as a multiplier to the Concentration from Curve value and the result reported in the Sample Stock Concentration field The Sample Stock Concentration results are reported in terms of the units select...

Page 61: ...the color coded boxes Some fluorophores do not exhibit significant fluorescence unless bound to a protein or nucleic acid It may be necessary to set up an assay reaction to best use the LED Wizard The standard curve assay methods use one excitation source to determine the concentration of a sample based upon the RFU measured using the corresponding emission filter set Advanced Options Use the Adva...

Page 62: ... it allows the user to excite a sample with one LED and get RFU results from multiple emission channels Quick Protocol 1 Launch the app from the Home screen 2 Select the Fluorophore type using the drop down menu on the Run screen Alternatively select an excitation LED using the radio buttons 3 Insert the standard 200 μL in a thin walled PCR tube into the FX mode sample holder 4 Optional Enter a sa...

Page 63: ...r example if the blue LED is selected the the emission results for the blue green and red channels will be reported If the green LED is selected only the green and red channel results will be reported New fluorophores may be entered using the List Add Edit button The Add button will pop up a window that includes a LED Wizard Tapping the Wizard button will result in the sample being excited by each...

Page 64: ... or comma number formats The DS 11 FX number format is determined by the language selected using the Settings app Export Formats Standard Formats The default csv file format for each absorbance measurement app includes all absorbance vs wavelength data for the app specific measurement wavelength range as well as the calculated data displayed on the app Report screen Custom Formats User specific fo...

Page 65: ...e Report screen Graphs are not exported as part of the csv file Use the Screen Capture option to export the current graph via email or to save the jpg image to a network folder or a FAT32 formatted USB flash Export Via Email Screen captures as well as data csv files may be exported via email Use the Account app Overflow Address Book function to save frequently used recipient email addresses It is ...

Page 66: ...cking frequency to Never on the Account Options screen The DS 11 email should be used only to export data and screen captures 5 Input Outgoing Server Information Use settings appropriate for the lab email server 6 Enter a name to display on outgoing messages Refer to your local IT support group if additional assistance is required Export to a Network Folder Screen captures data report csv files as...

Page 67: ...your local IT group to confirm that you have access to a local server or FTP site Ensure that the instrument is connected to WiFi or Ethernet 2 Launch the Accounts app and select a user account 3 Use the top drop down menu in the right pane to select the Add New Network Folder function 4 Use the List Add Edit button to add a network folder to the drop down menu 5 Select either the SMB or FTP optio...

Page 68: ...tures as well as data csv files may be saved to a FAT32 formatted USB flash drive The USB Flash Drive Eject icon appears in the bottom notification bar when a drive is detected The software will automatically safely unmount the flash drive after anything is written to the drive If not removed the software will automatically remount the flash drive when another write operation is performed Please w...

Page 69: ...When selected the auto export will apply to all measurement apps used by the applicable account Note All sample data is saved to the instrument even if automatic exports fail due to server connectivity issues Troubleshooting Tips Confirm that there is WiFi or Ethernet connectivity Use the Browser app on the second page of the Home screen to confirm connectivity or check the connectivity status ico...

Page 70: ... will display a preview of the print out along with many customizable options To select a printer ensure that the instrument has WiFi or Ethernet connection and then tap the first item listed under Printer Options This will bring up a list of available printers If no printers are listed tap the Network Printers icon in the left pane to search for nearby printers The current software supports appro...

Page 71: ...ual labels suitable for standard microfuge tubes o When used with the Colorimterics or Custom Standard Curves app the concentration printed is the dilution factor applied recalculated value A Quick Print button will be displayed on the Run Report and Graph screens when a DYMO printer is detected Label sizes may be selected by tapping the printer icon displayed next to the date and time in the bott...

Page 72: ...save frequently used recipient email addresses for easy reference System Accounts There are two preconfigured system accounts built into the software Primary Administrator This account is used to manage instrument settings It is not used within the measurement applications The primary administrator account is not initially password protected A password may be applied at any time Contact DeNovix if...

Page 73: ...t is deleted all associated sample and dye types wavelength lists and user methods are still available for general use unless deleted by an administrator Contact the holder of an administrator account if a standard user account password is forgotten Contact DeNovix customer support or your local distributor if the Primary Administrator password is forgotten Please have the serial number of the ins...

Page 74: ... may be archived from the Search Screen Search Options Data is automatically filtered based upon the Account selected using the tab on the top action bar The Advanced Search allows multiple filters to be applied in a single search The Quick Search option enables the user to filter by a single criteria in addition to the automatic Account filter Kinetics and Diagnostics results are not accessible u...

Page 75: ...ata app does not include a Graph screen for fluorescence apps Export vs Archive All data is automatically saved to the DS 11 FX onboard computer database at the time of measurement The Auto Export feature is available when a network folder is set up using the Accounts app The Export Selected Samples function available from the Report and Graph screen overflow menus creates a csv that can be emaile...

Page 76: ...s app When disabled only administrative accounts may archive data The storage capability of the DS 11 FX is sufficient for several years of data for a lab with average use Therefore it is recommended that the archiving function be used only as an infrequent method of deleting old data from the DS 11 database Although archiving a large amount of data may take a considerable amount of time the archi...

Page 77: ...n the Backup screen The restore function is used to recover user defined app settings methods dyes sample types email addresses shared folder paths user accounts and all sample results saved at the time of the backup There are two options Recover All Restores user defined app settings user entered methods user added dyes and sample types email addresses shared folder paths and saved user accounts ...

Page 78: ...a access control addresses can be found under this setting Permissions This selection is used to enable the following options Archive Use this selection to enable or disable all standard accounts including the General Account to archive delete data from the instrument Refer to the Export vs Archive section for additional information regarding archiving Automatic Updates Use this selection to enabl...

Page 79: ... Advanced Options checkbox 5 Select Static using the IP settings drop down 6 Enter the appropriate information and tap Save Ethernet To enter static IP addresses 1 Ensure the Ethernet cable is connected Select Ethernet from the Settings list 2 Tap the name of the connection Use the drop down menu to select Static Enter the appropriate information and tap Apply Note WiFi will take precedence over t...

Page 80: ...on ds11latest zip packages The yellow icon app uses only the Latest Software Version ds11latest zip package 2 Insert the USB device into one of the ports located in the back of the DS 11 3 Shut down all open apps 4 Swipe to page 2 of the Home screen and launch the Updater app Blue icon version of the app Press the Check for Updates button Follow the onscreen directions to Install the new yellow ic...

Page 81: ...is result may be copied and pasted into the Factor Input field used for nucleic acid concentration calculations in both the ssDNA and the Microarray apps Melting Temp For sequences up to 13 nucleotides the equation used is Tm wA xT 2 yG zC 4 where w x y z are the number of the bases A T G C in the sequence For sequences longer than 13 nucleotides the equation used is Tm 64 9 41 yG zC 16 4 wA xT yG...

Page 82: ...ts are available However conversions between the two types of units are not enabled Timer App This app is a lab tool that allows a user to set two independent timers At the end of a specified period an alarm will sound for a maximum of 90 seconds Push the Off button to silence the alarm The alarm can be acknowledged via the notification bar or by going back to the Timer app The Timer will work in ...

Page 83: ...e bottom sample surface Lower the upper arm Wait 1 2 minutes 2 Wipe away the water from both the upper and lower sample surfaces with a dry lint free lab wipe Between Measurements Use a dry lint free lab wipe to remove samples from both the lower and upper sample surfaces Additional Cleaning Use the following procedure to remove sticky solutions e g proteins that may have dried down on the sample ...

Page 84: ...t may damage the internal components of the instrument Refer to the cuvette manufacturer for guidance on cleaning cuvettes Solvent Compatibility The DS 11 spectrophotometer microvolume sample surfaces are compatible with most solvents typically used in life science laboratories Exception Do not use Hydrofluoric Acid HF as the fluoride ion will dissolve the quartz fiber optic cable Customer Support...

Page 85: ...1 models remove cuvettes from the holder block and ensure the light path is clear of all obstructions prior to starting the Self Test cuvette mode assessment A spectral plot of the xenon flash lamp intensity and a table that compares measured versus specified values for a panel of specifications will be displayed If any of the parameters fail to meet specifications clean the measurement surfaces a...

Page 86: ...avigate to the Verification tab 3 Enter the lot specific Target Absorbance stated on the ampule in the appropriate field 4 Add 1 μL of dH2O to the pedestal lower the arm and click Blank 5 Wipe the upper and lower pedestals using a dry lint free laboratory wipe 6 Vigorously shake the nicotinic acid ampule to thoroughly mix the solution Ensure all of the solution is in the bottom portion of the ampu...

Page 87: ...e a fresh tip to pipette each aliquot onto the sample surface The sample surfaces are not adequately cleaned between measurements Wipe both the top and bottom surfaces with a dry lab wipe The solution has concentrated due to prolonged exposure Use a fresh ampule of solution High Standard Deviations Multiple measurements made using a single 1 ul aliquot Use a fresh aliquot for each measurement Pipe...

Page 88: ...the verification check No other sources of nicotinic acid are tested and validated for use with the DS 11 verification procedure Results A Pass or Fail message will appear in the results box at the bottom of the screen If the instrument fails please clean the surfaces and repeat the test with a fresh aliquot of LC NA Technical Support Contact DeNovix Customer Support if additional help is required...

Page 89: ...operly Results will be reported as Pass Fail Technical Support Contact DeNovix Customer Support if additional help is required For US customers please email the results directly to info denovix com using the FX Diagnostics app Overflow Tech Support function Include your name phone number institute company and return email address Please note responses will not be sent directly to the instrument em...

Page 90: ...of the instrument In the case of fluorescence assays ensure sample concentrations fall within the reagent limits as described by the assay manufacturer Microvolume Mode Ensure both top and bottom microvolume measurement surfaces are clean prior to making the Blank measurement Always remove sample solutions from both the top and bottom measurement surfaces using dry lint free wipe immediately after...

Page 91: ...cing air bubbles into the sample solution when mixing samples Ensure all sample concentrations in the thin walled assay tubes fall within the limits of the assay or reagent kit for accurate results Follow assay reagent manufacturers recommendations regarding temperature incubation time and protection from ambient light Follow assay reagent manufacturers recommendations regarding suggested standard...

Page 92: ...st cases simply relaunching the app will allow the user to continue to make measurements Update the software using the Updater app if experiencing a reoccurring software error Contact DeNovix Customer Support 302 442 6911 or info denovix com if additional help is required Please include the serial number of your instrument when contacting Customer Support by email The serial number may be found on...

Page 93: ...he instrument Outside of the US please contact your local distributor for assistance Warranty All product s and accessories sold by DeNovix Inc are under warranty against manufacturing defects in parts Disclaimer All information in this document is for reference purposes only DeNovix Inc makes no claims that this document is complete or error free and assumes no responsibility and will not be liab...

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