Purified Protein, Peptides: Absorbance
Protein A280
The Protein A280 application is used to quantify samples based upon
absorbance values at 280 nm.
Screen Features
•
Sample Type: The sample type and associated mass extinction coefficients
options include:
Use the 1A=1mg/ml option when neither the E1% nor Ext Coeff and MW for the
purified protein sample are known.
•
Concentration Results: Results are reported in units of mg/mL.
• Units may be changed using the Run screen top action bar Overflow
menu. The units selected at the time of the measurement is what is saved
to the database.
•
A280: Reports the baseline-corrected 280 nm absorbance value.
•
260/280: Displays the ratio of the 260 and 280 nm absorbance values.
Protein Type List
New Protein types using either user-defined E1% or MW and Ext. Coeff values
may be saved using the List Add/Edit button to the right of the sample type drop-
down menu.
•
The new sample types may be used by any account holder.
•
The new sample types may be edited or deleted only by the account holder or
an administrator account.
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Summary of Contents for DS-11 FX+
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