DAKEWE 6250 User Manual Download Page 1

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Revised Version V4.1-English Version-June 2021
Dakewe(Shenzhen)Medical Equipment Co,. Ltd

V4.1

Cryostat

User’s Manual

Applicable Model:



6250

Summary of Contents for 6250

Page 1: ...Revised Version V4 1 English Version June 2021 Dakewe Shenzhen Medical Equipment Co Ltd 1 V4 1 Cryostat User s Manual Applicable Model 6250 ...

Page 2: ...ed this User Manual to the instrument which includes the instrument s structural features technical characteristics instructions for use simple troubleshooting maintenance etc For your ease of use this instrument Before use be sure to read the User Manual carefully and follow the instructions correctly to the user manual In addition if you have any questions during use contact technical support we...

Page 3: ...ns etc contained in this Manual organizations or personnel are encouraged to give us feedback for updating It is also important to note that we shall not be liable for any direct or indirect economic loss or damage caused by non compliance with statements or other information in this Manual This document is protected by copyright law and all copyright in this document belongs to Dakewe Shenzhen Me...

Page 4: ...ations 13 3 1 Overview 13 3 2 Technical Parameters 14 4 First Installation 16 4 1 Installation Requirements 16 4 2 Unpacking Tools 16 4 3 Unpacking 17 4 4 Accessory Installation 18 4 4 1 Hand Wheel Installation 18 4 4 2 Hand Wheel Block Installation 19 4 4 3 Unlock the Instrument Cover 19 4 4 4 Fixed Card Core 20 4 4 5 Waste Trough Installation 20 4 4 6 Blade Holder Installation 21 4 4 7 Glass Doo...

Page 5: ...ectioning Operation 36 6 1 Starting the Instrument 36 6 2 Preparing Sectioning Tools Specimen Disc and Auxiliary Preparation Tools 36 6 3 Retouching Slice 36 6 4 Sectioning 37 6 5 Sectioning Reference 37 7 Cleaning and Maintenance 38 7 1 Daily finishing work 38 7 2 Long term Suspension of Finishing Work 38 7 3 Cleaning Instrument 38 7 3 1 Before each cleanup perform the following steps first 38 7 ...

Page 6: ...ious injury Note Indicating information that is not related to risk but is very important Follow this User Manual Note that see the User Manual for relevant warning information IVD medical device In accordance with appendix four of directive 2002 96 EC of the European Parliament and Council Power on Power off Biohazard warning black line on yellow background Sign indicator of using flammable solve...

Page 7: ...llowable limit Serial number Manufacturing date Manufacturer Expiration No stacking Limited height of 2 floors accessory cases only Indicating the correct vertical position of the packing container Goods are fragile and must be handled with care The package must be stored in a dry environment Recycle in accordance with local laws and regulations It meets the requirements of EU Directive 98 79 EC f...

Page 8: ...dical colleges by legal medical experts and propagation institutes It is used exclusively by pathology laboratories and is limited to the following tasks Only operate the instrument as described in this Manual Any other ways of use are regarded as inappropriate 1 3 Working principle After the operator quickly freezes the fresh tissue hard the frozen hard tissue is placed in a precision controlled ...

Page 9: ...ll safety precautions and warnings in this User Manual Always observe the safety and precautions described in this Chapter Even if you are already familiar with the operation and use of other Dakewe products be sure to read these instructions Do not remove or modify the protective devices on the instrument and accessories Only Dakewe certified qualified maintenance personnel can repair the instrum...

Page 10: ...Only authorized maintenance personnel are allowed to repair the instrument and dispose of the instrument s internal components The warning triangle is displayed on the instrument to ensure that correct operating instructions defined in this Manual must be observed during operation and replacement Failure to follow these instructions may result in accidents personal injuries and damages to the inst...

Page 11: ...e sample block first then clamp the blade The handlebar must be locked and the blade must be guarded with the blade guard during operation or replacement of the blade and sample and during the rest periods Be sure to wear safety goggles when making fragile sample sections Sample may be fragmented Make sure that liquid does not enter the inside of the instrument during operation Immediately picking...

Page 12: ... power to ensure that it conforms to the specification requirements on the instrument nameplate refer to figure 1 After confirming that the power supply has a grounding wire three hole socket connect the unit and the power socket with the supplied power cord and turn on the power switch on the right of the machine to enter the working state Keep the hand wheel in the locked position if the machine...

Page 13: ...pecifications 3 1 Overview 1 Display 2 Keyboard 3 Waste barrel 4 Foot cup 5 Power switch 6 Glass door 7 Hand wheel 8 Sample disk 9 Hammer 10 Specimen clamp 11 Tool holder 12 Front brush holder 13Brush Figure 2 Front picture of 6250 Cryostat Figure 3 The picture of cooling chamber ...

Page 14: ...it mm Excluding hand wheel Size without packaging width depth height unit mm Including hand wheel 650 700 1280mm 5 780 700 1280 mm 5 Empty weight without packaging 140kg Weight with packaging 170kg Section Parameter Section thickness range 0 100μm Section step amount 0μm 20μm with a step of 1μm 20μm 50μm with a step of 2μm 50μm 100μm with a step of 5μm Section thickness deviation 20 Trimming thick...

Page 15: ...μm s Cooling Parameter Freezing chamber s operating temperature 35 40 adjustable Freezing shelf s operating temperature 40 40 Defrost Manual defrosting automatic defrosting Minimum reaching temperature of semiconductor rapid cooling area 60 5 Number of common freezing points Number of semiconductor fast cooling points Refrigerant 18 2 R449A 400g 5g Cooling capacity 690W Refrigerant and compressor ...

Page 16: ...th the feet so as to avoid instability of the instrument to affect the quality of the sections Avoid vibration direct sunlight and sudden temperature changes The installation site must be well ventilated and no fire source is allowed 4 2 Unpacking Tools 1 Electric screwdriver 2 Adjustable wrench 3 Utility knife 4 Allen wrench Always wear protective gloves for installation and removal to avoid cuts...

Page 17: ...wrench to unscrew the remaining screws of the wooden box remove the four side planks and the surrounding foam and remove the wrapped packaging film to move the carton with the accessories to the ground Place it smoothly Figure 5 3 4 Placing it on the ground then rotate the left and right foot cups counterclockwise and push the device roller rolling to the installation position The wooden box can b...

Page 18: ...ction of the arrow to observe that the universal specimen clamp in the freezing chamber shall move along with it Place the hand wheel handle at the highest position push the hand wheel lock lever into the bottom of the hand wheel groove If it is not locked turn the hand wheel slightly until the locking mechanism engages The hand wheel can only be locked at the highest and lowest position and the r...

Page 19: ... Use the M5 Allen key to loosen the two top cover fixing screws Figure 8 1 After the screw is completely loose flip up the top cover to unlock Upper cover fixing screw position Figure 7 The procedure of hand wheel block installation Figure 8 The picture of upper cover fixing screw position The top cover can be opened by loosening the screw for 5 8 cycles and the screw does not have to be taken out...

Page 20: ...e whole machine is placed in place lock the handwheel at the highest position buckle the bottom of the supporting EVA cotton and take out the EVA cotton The chuck core can move up and down normally 4 4 5 Waste Trough Installation Place the waste trough in the body of the universal specimen clamp in the direction of the illustration Figure 10 Figure 9 The picture of card core fixing Figure 10 The p...

Page 21: ... direction of the blade holder s open dovetail slot Figure 11 2 Slide to the appropriate position then rotate the handle to lock the blade holder 4 4 7 Glass Door Installation Open slot Blade holder front and rear movement locking handle Blade holder mounting plate Figure 11 The procedure of blade holder installation Figure 12 The procedure of glass door installation Conductive sheet Conductive el...

Page 22: ...lose the upper cover and lock it 4 4 8 Power Supply Cable Connection This unit comes with a power cord that connects the unit to a power outlet Precautions The voltage supplied by the power socket must match the voltage indicated on the slicer s nameplate 4 5 Boot Preparation After transporting and moving the machine must be stand for more than 2 hours before starting up for the first time Check t...

Page 23: ... the box 3 Compressor work instruction The and buttons are used to set the temperature adjustment The setting range is 35 C to 40 C When the compressor stops for less than 3 minutes and receives the signal to start the compressor again the compressor will not start immediately it will be delayed until the stop time reaches 3 minutes before starting Figure 13 The picture of working interface Figure...

Page 24: ...ing countdown time 3 It is semiconductor cooling After the touch icon the semiconductor cooling is turned on the turn on time is 300s and the countdown is automatic When the value is 0 the semiconductor cooling is automatically turned off and the icon is touched before turn off and the semiconductor cooling function is closed The semiconductor cooling chip switch must meet two conditions 1 cold st...

Page 25: ...n to end the drying mode and enter the normal cooling state Precautions 1 The drying function can only be turned on when the glass door is open When the glass door is closed when the drying function is running the program will automatically end the drying mode and return to normal cooling mode 2 Before drying is turned on all samples in the freezer must be emptied 3 Drying is only suitable for a l...

Page 26: ... The chuck core advances slowly and the position icon moves slowly to the Front direction Press this key to switch the sectioning retouching mode cyclically Thickness value adjustment The chuck core moves back quickly and the position icon moves quickly in the Back direction The card head core moves forward quickly and the position icon moves quickly to the Front direction 5 3 Function Setting and...

Page 27: ...o display Choosing the hour and minute Press the number on the bottom right to increase and decrease the time Press the Save button to save the settings and press Cancel button to abandon the settings Precautions 1 Each defrost time is 15 minutes by default or the box temperature rises to 5 C during the defrosting process the defrosting will end in advance 2 After finishing defrosting it will auto...

Page 28: ...the bottom right to increase and decrease the time Press the Save button to save the settings and press Cancel button to abandon the settings Precautions 1 When the sleeping time ends the device enters the normal working state the device automatically starts the compressor and cools to the specified box temperature and station temperature After setting the time press the Save button to save otherw...

Page 29: ...d the disinfection will be turned off 3 If the timed automatic disinfection is also performed during the manual opening of the disinfection it will not stopped after 35 minutes but will stopped at the end of the timed automatic disinfection After setting the time press the Save button to save otherwise the setting is invalid 5 3 4 Time reset in the function interface Double click the icon Reset in...

Page 30: ...e and icons to adjust the value the first two 20 of the year cannot be modified 3 Press the Save button to save the set time and the system time on the working interface will be changed synchronously 5 5 2 Slice Retraction Value Setting Press the button and to increase or decrease the retraction stroke The retraction stroke range is 0 100um Each time you press and decrease the value increases or d...

Page 31: ... knob 2 Anti roll plate locking knob 7 Blade pressing plate 3 Blade clamping handle 8 Anti roll plate s flip handle 4 Holder locking handle for 9 Anti freeze hand rubber stopper left and right movement 5 Holder locking handle for 10 Blade slicing angle locking screw back and forth movement Figure 24 The picture of blade holder description ...

Page 32: ...lade holder left and right with hands Lock at the selected position 5 6 3 Blade Installation or Replacement Loosen counterclockwise rotation the blade clamping handle to widen the gap of the blade pressing plate Push the blade withdrawal pusher to take out the blade Then insert a new blade from right to left turn the blade locking handle to allow the blade to be fixed by pressing plate and the bla...

Page 33: ... 2 When you feel that the quality of the cut tissue is not high you should check the blade and the anti roll plate for any tissue fragments grease and other foreign matter 3 Avoid touching the edge of the anti roll plate with your hand or other hot objects otherwise the tissue pieces will stick as the temperature rises 5 6 5 Microtome Blade Angle Adjustment Use a hex wrench to loosen counterclockw...

Page 34: ...ition After the tissue on the carrier head is frozen the shaft of the carrier head is inserted into the hole in the center of the chuck core so that the rear surface of the carrier head is in close contact with the front surface of the chuck core and the rotation of the carrier head set screw will be It is locked to the front of the card core Loosen the head adjustment lock screw on the core of th...

Page 35: ...the freezer is always lower than the temperature in the freezer At normal temperature cut the specimen into the appropriate size and place it on the carrier head and position it with the embedding agent Insert the Sample disk with sample into the hole of the freezer and freeze it at low temperature During specimen freezing a heavy hammer can be placed on the specimen surface to accelerate the free...

Page 36: ...cing pay attention to the following operations Put the pre cooled blade into the tool holder and lock it adjust the anti roll plate Appropriately adjust the slice angle of the tool holder about 4 6 The slicing angle has been adjusted generally no need to adjust Adjust the relative position of the blade holder and the specimen to leave room for the infeed stroke Open the anti roll plate Open the ha...

Page 37: ...anti rolling plate some of which affect each other and need to be carefully debugged they cannot be placed on the blade 2 When living tissue is frozen the water condenses into ice the tissue becomes hard and its hardness changes with temperature The lower the temperature the harder the tissue whichever tissue at whatever temperature whether they can obtain good quality slice need to be explored th...

Page 38: ...g waste Remove the waste tray the front brush holder and blade holder for cleaning and disinfection Precautions Before the instrument is restarted it must be checked whether all parts of the sliced movement in the freezer are completely dry 7 3 Cleaning Instrument Generally alcohol or alcohol containing conventional disinfectants can be used for cleaning It is strictly forbidden to use acetone xyl...

Page 39: ...ning the contact surface between the two After cleaning dry the parts and apply low temperature lubricating oil on the contact surface of the tool holder seat and the rotating tool seat Install and restore in order 7 4 General Maintenance Clean the inside of the machine freezer every day Lubricate the specimen feeding mechanism every week press the fast forward button to move the specimen chuck to...

Page 40: ...t brush holder cold table decoration plate and Sample disk Remove the relevant parts carrier chuck front panel decorative panel temperature sensor hood Remove four screws M8X25 with a hex wrench Loosen the locking screw of the coupling with a 3mm hexagon wrench Lift the core with two hands pull it out from the positioning pin and then move it slightly to the left to disengage the coupling axially ...

Page 41: ...ction Precautions Before replacing the fuse the main switch of the machine must be switched off and the power plug must be unplugged The insurance specification is 4A the size is 5 20mm only the fuse of the same specification can be used Otherwise it may cause serious damage to the equipment 7 6 2 Replacing the UV Lamp There is a light tube in the freezing room and the ultraviolet germicidal lamp ...

Page 42: ...ow is the most common list of questions that may arise when using the instrument The table lists possible causes and solutions to these problems Fault Cause Solution Tissue blocks fall off Inadequate freezing embedding agent Increase the amount of embedding agent The trimming feed is too large Use the trimming mode of the device to trim and set the trimming thickness to 20 40um Section softening m...

Page 43: ... thickness is uneven even the knife skips during the slicing process The sample holder is not fixed Tighten the sample tray locking screw The trimming blade is too large the tissue is loose on the sample and the freezing is not firm Refreeze tissue with embedding agent The blade is loosen Lock the blade lock handle The blade holder is loosen Lock the blade holder seat locking handle Damaged blade ...

Page 44: ...effects Remove static electricity Anti roll plate temperature is too high Lower the temperature The sample holder is frozen and glued on the cold table Before freezing there is moisture at the bottom of the sample holder Remove the heavy hammer and hit the sample holder The cold table temperature is also low and the speed of freezing the tissue becomes slower There is foreign matter between the sa...

Page 45: ...ey 15 25 Lips 15 25 liver 15 25 lung 25 35 Lymph nodes 15 25 muscle 15 25 bladder 15 25 Ovaries 15 25 pancreas 15 25 prostate 15 25 skin 15 25 Skin fat 30 35 spleen 15 25 tongue 15 25 thyroid 15 20 Uterine scraping 10 15 Cartilage 10 15 Caution The temperature given in the above table is a summary of long term experience but these are only approximate values because specific adjustments may be req...

Page 46: ...For special warranty services contact your local sales representative or the dealer who sells the instrument 10 2 Technical Service Information If you need technical services or component replacement contact your local Dakewe s sales representative or dealer who sells the instrument Please provide the following information Model name and serial number of the instrument Instrument location and cont...

Page 47: ...vation Industrial Park No 14 Jinhui Road Kengzi Street Pingshan New District Shenzhen Production Address Room 601 Building 1 Shenzhen Bio pharmaceutical Innovation Industrial Park No 14 Jinhui Road Kengzi Street Pingshan New District Shenzhen Product Recordation Number Y S X B 20180097 Production Recordation Certificate Number Y S S Y J X S C B 20160031 Tel 0755 26413421 Fax 0755 27383156 Customer...

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