Instruction Manual | 11
• The pipettor should be held in a vertical position.
• Change the pipet tip when volume setting is changed or
when a different liquid is to be aspirated.
• Change the pipet tip if a droplet remains on the end of
the tip from the previous pipetting operation.
• Each new pipet tip should be pre-rinsed with the liquid
to be pipetted.
• Liquid should never enter the pipettor shaft. To prevent this:
- Press and release the pushbutton slowly and smoothly.
- Never turn the pipettor upside down.
- Never lay the pipettor on its side when there is liquid
in the tip.
• Never force the volume setting beyond its
recommended limits.
• When pipetting liquids with temperatures different
from the ambient temperature, it is recommended to
pre-rinse the tip several times before use.
• Do not pipette liquids with temperatures above 70°C.
• When pipetting acids or corrosive solutions which emit
vapors, it is recommended to disassemble the shaft
and rinse the piston and seal with distilled water after
finishing the pipetting operation.
8. Recalibration
The accuracy and precision of the pipettors are obtained
gravimetrically using Axygen® pipet tips, performing at
least 10 measurements of distilled water according to
EN ISO 8655 standard.
If during pipettor operation you find that the accuracy
error (the difference between the real aspirated volume
and the preset volume) exceeds the permissible value
given in the table in Section 1, the pipettor recalibration
procedure should be carried out.
Before starting the recalibration it is necessary to check
whether the following requirements have been met
during error determination:
• Ambient temperature and the temperature of the
pipettor, pipet tips, and water were identical.
• Density of the liquid used is close to that of distilled water.
• Balance with appropriate sensitivity has been used.
• Conversion factor mg/μL has been taken into account.
• The requirements shown in Sections 3 and 7 have
been met.
