CORNING Axygen Axypet Instruction Manual Download

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A Corning Brand

Axygen® Axypet®

Pipettors

Single-channel, 8-channel,

and 12-channel Pipettors

Instruction Manual

Summary of Contents for Axygen Axypet

Page 1: ...A Corning Brand Axygen Axypet Pipettors Single channel 8 channel and 12 channel Pipettors Instruction Manual...

Page 2: ...3 Aspiration and Dispensing Instructions 8 4 Pre rinsing 9 5 Dense and Viscous Liquids 10 6 Axygen Pipet Tips 10 7 Recommendations 10 8 Recalibration 11 9 Troubleshooting 15 10 Cleaning and Sterilizat...

Page 3: ...ranges 0 5 to 10 L 5 to 50 L 20 to 200 L 50 to 300 L Model Volume Range L Application 2 10 Measurement and transfer of micro volumes DNA sequencing and enzyme assay applications 20 50 100 200 250 300...

Page 4: ...lity of cross contamination of the liquid samples To protect the user from contaminated pipet tips the pipettor is fitted with a built in tip ejector D Single channel Pipettor Ejector The ejector leng...

Page 5: ...to 5 mm If the ejector adjustment described above is not sufficient or the diameter of the ejector opening is too large to eject the tip it will be necessary to put the ejector cap M onto the ejector...

Page 6: ...volume depends on the quality of pipet tips used The values for accuracy and precision shown in the table below were obtained using Axygen pipet tips Model Volume Range L Cat No Volume L Accuracy Pre...

Page 7: ...0 1 5 200 100 1 5 0 8 Max 200 1 0 0 6 8 300 12 300 AP 8 300 AP 12 300 Min 50 1 6 1 5 300 150 1 2 1 0 Max 300 1 0 0 6 The accuracy and precision are obtained gravimetrically using Axygen pipet tips pe...

Page 8: ...L Counter Readings Set Volume Increment Single channel Pipettors 2 1 2 5 1 25 L 0 002 L 10 0 7 5 7 5 L 0 02 L 20 1 2 5 12 5 L 0 02 L 50 0 3 8 38 0 L 0 2 L 100 0 7 5 75 0 L 0 2 L 200 1 2 5 125 0 L 0 2...

Page 9: ...lue adjust the value down to a one third turn above the required setting If the desired value is higher than the previous value adjust the value to a one third turn above the required value The settin...

Page 10: ...ct approximately 1 5 mm into the manifold The suspension system of the shafts ensures even and tight sealing of the pipet tips The rocking movement does not have to be performed to seal the pipet tips...

Page 11: ...ters A replaceable filter L fitted in a seat in the bottom part of the shaft is used in 5 000 L and 10 000 L pipettors This filter prevents the aspirated liquid from entering into the shaft and thus f...

Page 12: ...ff from the nominal value Correction value 2 x nominal val m weight of the sample density of liquid Check this operation once again and correct if necessary Note the corrected value for further pipett...

Page 13: ...ns which emit vapors it is recommended to disassemble the shaft and rinse the piston and seal with distilled water after finishing the pipetting operation 8 Recalibration The accuracy and precision of...

Page 14: ...5 C and stabilized during weighing within 0 5 C Measurements should be conducted using distilled water Balance sensitivity should be suitable for the volume to be measured Recalibration Procedure Set...

Page 15: ...aspirations weigh each one and calculate the average value of the aspirations Perform 3 aspiration series each series should include the aspirations from all channels weigh each time and calculate th...

Page 16: ...ll separate 2 Holding the volume setting knob to protect it against rotation insert the calibration key into the cuts of the calibration screw 3 Turn the calibration key clockwise to reduce the aspira...

Page 17: ...acement of parts should be required only occasionally and should not occur under normal pipettor use Problem Cause Solution Droplets of liquid remain in the pipet tip The pipet tip is emptied too fast...

Page 18: ...ored Remove the tip ejector Unscrew the shaft nut and inspect the shaft and the piston assembly Replace the damaged parts When reassembling the pipettor the nut should be hand tightened In the single...

Page 19: ...o lubricant on the sealing elements Remove the tip ejector Unscrew the shaft nut and remove the shaft piston assembly seal and O ring Rinse the removed parts in distilled water and dry thoroughly Ligh...

Page 20: ...rilization and re tighten after autoclaving Set the locking ring in lower unlocked position prior to sterilization Sterilize the pipettors in autoclave with an initial vacuum and drying cycle Prior to...

Page 21: ...ters for pipettor models AP 5000 AP 10ML Lubricant for single channel pipettors Color identification rings for single channel pipettors Right hand ejector button Cat No Description AP USD Pipettor sta...

Page 22: ...hase CORNING DISCLAIMS ALL OTHER WARRANTIES WHETHER EXPRESSED OR IMPLIED INCLUDING ANY IMPLIED WARRANTIES OF MERCHANTABILITY OR OF FITNESS FOR A PARTICULAR PURPOSE Corning s sole obligation shall be t...

Page 23: ...xclusion or limitation of incidental or consequential damages This warranty gives you specific legal rights You may have other rights which vary from state to state No individual may accept for or on...

Page 24: ...an t 886 2 2716 0338 EUROPE CSEurope corning com France t 0800 916 882 Germany t 0800 101 1153 The Netherlands t 20 655 79 28 United Kingdom t 0800 376 8660 All Other European Countries t 31 0 206 59...