Cleaver Scientific MSSCREEN-TRIO Instruction Manual Download Page 1 | Manualshive

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Summary of Contents for MSSCREEN-TRIO

Page 1: ...multiSUB Screen Horizontal System...

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Page 3: ...RIO MSSCREEN16 NC MSSCREEN24 NC MSSCREEN32 NC MSSCREEN TRIO NC Record the following for your records Model _____________________ Catalogue No _____________________ Date of Delivery ___________________...

Page 4: ...ons 6 Setting up the Horizontal Gel Tanks 6 Fitting Electrodes 6 Fitting Loading Guides 6 Gel Preparation 7 Gel Pouring 8 Flexicaster 8 Tape 9 Running the Gel 9 Gel Staining and Viewing 10 Solutions 1...

Page 5: ...he complete manual thoroughly The unit must never be used without the safety lid correctly in position The unit should not be used if there is any sign of damage to the external tank or lid These unit...

Page 6: ...x 16cm W x L 6 x MS26 28MC 1 1mm 28 sample MS26 LG Strips MS26 WP Platform CSL CAB None MSSCREEN24 NC MS26 UV24 26 x 24cm W x L 6 x MS26 28MC 1 1mm 28 sample MS26 LG Strips MS26 WP Platform CSL CAB No...

Page 7: ...ions Power supply cables 1500V rated with retractable 4mm connectors Safety lid and viewing pane High impact acrylic construction Height adjustable combs Acrylic UV transparent gel tray UV transparent...

Page 8: ...Fitting Electrodes Note the position of the lid on the unit This shows the correct polarity and the correct orientation of the cables black is negative and red positive Remove the lid from the unit N...

Page 9: ...ose powder to a conical flask 2 Add the appropriate amount of 1x TAE or TBE solution from the table above To prevent evaporation during the dissolving steps below the conical flask should be covered w...

Page 10: ...end of the Flexicaster 3 Position the movable end of the Flexicaster so that the silicone mat is pushed against the other end of the tray 4 Turn the cam so that the silicone mat tightly seals against...

Page 11: ...ip 4 Allow the agarose to set ensuring that the gel remains undisturbed 5 Carefully remove the gel casting gates and comb and transfer the gel including tray to the main tank Running the Gel 1 Mix the...

Page 12: ...sed 3 Rinse the gel twice for a couple of seconds with distilled water 4 Transfer the gel to a UV Transilluminator The samples will often appear as brighter clearer bands when photographed or viewed u...

Page 13: ...lycerol 0 25 bromophenol blue and 0 25 xylene cyanole FF in 1x TAE buffer Only 1 10 ml of the 10x loading dye should be prepared Ethidium Bromide Solution Add 10 mg of Ethidium Bromide to 1 ml distill...

Page 14: ...running surface Curved line or distortion of bands Bubbles in sample wells Remove bubbles prior to electrophoresis Curved bands smiles Sample overload Reduce load Differential relative mobilities Sam...

Page 15: ...gents include dishwashing liquid Hexane and Aliphatic hydrocarbons The units should not be left to in detergents for more than 30 minutes The units should never come into contact with the following cl...

Page 16: ...C 0 75mm thick MS26 56MC 0 75 Comb 56 sample MC 0 75mm thick MS26 28MC 1 Comb 28 sample MC 1mm thick MS26 56MC 1 Comb 56 sample MC 1mm thick MS26 28MC 1 5 Comb 28 sample MC 1 5mm thick MS26 56MC 1 5 C...

Page 17: ...5g tablets CSL RUNSAFE CSL RUNSAFE Package 1 ml vial CSL TBEP Powdered Tris Borate EDTA Running Buffer 10 sachets 1litre pk TBE10X1L Cleaver Buffer Tris Borate EDTA Running Buffer 10 x 1L TBE10X5 Cle...

Page 18: ...n performed by anyone other than CSL or an appointed distributor or representative are no longer under warranty from the time the unit was modified Units which have accessories or repaired parts not s...

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