15/11/2018
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2. Remove the tray.
3. Peel the back off the loading guide and carefully apply the loading
guide directly to the gel platform.
The unit is now ready to be used.
Gel Preparation
The Table below shows the volume of agarose solution required to make the
desired agarose gel for each unit tray size. For a standard 0.7% agarose gel,
add 0.7 grams of agarose to 100 ml of 1x TAE or TBE solution. The same 1X
solution should be used in the tank buffer solution.
Tray
Gel volume for a 5mm thick gel
26 x 16cm
208ml
26 x 24cm
312ml
26 x 32cm
416ml
1. Add the agarose powder to a conical flask.
2. Add the appropriate amount of 1x TAE or TBE solution from the table
above. To prevent evaporation during the dissolving steps below, the
conical flask should be covered with parafilm.
3. Dissolve the agarose powder by heating the agarose either on a
magnetic hot plate with stirring bar or in a microwave oven. If using the
microwave method, the microwave should be set at around a 400 watt
or medium setting and the flask swirled every minute. The solution
should be heated until all crystals are dissolved. This is best viewed
against a light background. Crystals appear as translucent crystals.
These will interfere with sample migration if not completely dissolved.
The gel must be cooled to between 50°C and 60°C degrees before pouring.
