15/11/2018
Page 8
Gel Pouring
The multiSUB
®
range of units allows two different methods of gel casting:
1. Flexicaster
2. Traditional Tape
Flexicaster
1. Level the Flexicaster base by adjusting the feet so that the bubble is
exactly central.
2. Insert the desired length tray into the Flexicaster such that one end of
the tray is pushed up and seals against the silicone mat of the
permanent end of the Flexicaster.
3. Position the movable end of the Flexicaster so that the silicone mat is
pushed against the other end of the tray.
4. Turn the cam so that the silicone mat tightly seals against the side of the
tray. Pour in the agarose carefully so as not to generate bubbles. Any
bubbles that do occur can be smoothed to the edge of the gel and
dispersed using a pipette tip.
5. Allow the agarose to set, ensuring that the gel remains undisturbed.
6. Carefully remove the gel casting gates and comb and transfer the gel
including tray to the main tank.
MS7-FC and MS20-FC Flexicasters
