ChemoMetec 900-0300, User Manual

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2 Introducing the NucleoCounter
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A fluorescent image of the content of the measurement chamber is thus . Example
of such an image is shown in Figure 4. Each spot in the image represents a cell
containing DNA stained with PI.
Figure 4 . Fluorescent image (from NucleoView PC software) of cell DNA contained
in the measurement chamber of a NucleoCassette
2.1.2 Built-in image analysis
The NucleoCounter is equipped with advanced software for image analysis. This
integral software analyzes the recorded image and counts the num ber of nuclei in
the image. As the volume of the cassette measurement chamber and the dilution of
the lyzate is known, the cell density in the initial sample can be calculated. The
concentration of cells in the initial sample is presented in the NucleoCoun ter
display. If the optional software (NucleoView) is used, the results and the recorded
fluorescent image can be viewed.
The entire process takes approximately 30 seconds, from pressing “ Run” to the
presentation of the result in the display of the NucleoC ounter.
Performing a cell count with the NucleoCounter is objective and the results are
independent of the operator.
2.2 The NucleoCassette
The NucleoCassette is a custom made disposable plastic device designed for
optimal sample handling and safe disposal. The cassette is shown in figure 5. The
main features of the NucleoCassette are the piston, the flow system containing the
fluorescent dye, propidium iodide, and the measurement chamber.