ChemoMetec 900-0300, User Manual

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2 Introducing the NucleoCounter
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2 Introducing the NucleoCounter YC -100
The NucleoCounter method provides a fast alternative to the manual techniques,
yielding objective and reliable results. Furthermore the NucleoCounter technology
provides improved safety to the user compared to other com parable methods.
The NucleoCounter system is comprised of the NucleoCassette for sample handling,
the NucleoCounter instrument for particle analysis and Lysis buffer for cell lysis
(please refer to appropriate Application Notes provided by ChemoMetec conce rning
lysing of cells). Basics of the NucleoCounter system is explained on the next few
pages.
The NucleoCounter system identifies and counts individual cell containing stained
DNA. The sample pre-treatment is intended to allow the effective staining of c ellular
DNA but it will also aid the dissolving of cell aggregates. Depending on cell species
and sample properties some additional effort might be needed to dissolve cell
aggregates (please refer to appropriate Application Notes provided by
ChemoMetec).
2.1 The NucleoCounter YC-100 instrument
The NucleoCounter is shown in the figure below.
Figure 2. NucleoCounter instrument
Refer to chapter 5 with respect to the operation of the instrument.
Lid
Control Buttons
Keypad
Display
Weight 3 kg
Height
26 cm
Width 38 cm
Depth 22 cm