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Boeco TC-PRO-3048 Instruction Manual Download Page 17

 

BOECO TC-PRO 

Thermal Cycler                                                                    Chapter Three Preparations 

—

  9 

—

 

 

Notes: 

The fuse specifications are as follows: 250V 8A, 

Φ

5

×

20mm. The fuse should be 

replaced by one that meets those specifications.    If you need certificated fuses, 
please contact the distributor or manufacturer.   

 
 

2. Keyboard Diagram 

 

 
 

3. Keyboard Instructions                                                                               

 

                                                                                       

 
 
 
   

“Digit  &  Letter”,

  select 

the  letter  by  pressing  in 
succession. 

 

Summary of Contents for TC-PRO-3048

Page 1: ...THERMAL CYCLER TC PRO Instruction Manual...

Page 2: ...manual All rights reserved Any part of this manual shall be forbidden to photocopy reproduce or translate into other languages without written permission from the Boeckel Co GmbH Co KG Document Numbe...

Page 3: ...793 1 is a general instrument of class the protection degree is IP20 It is intended for indoor use a Grounding Considerations A C power s grounding should be grounded reliably for fear of an electric...

Page 4: ...device s interior from being too hot When a single device is running the shortest distance between its openings and the nearest object is 50cm when two or more devices are running at the same time th...

Page 5: ...the program execution and in the short period after the program execution the metal part near those signs on the block and hot lid is not allowed to be touched by any part of the body for fear of the...

Page 6: ...acking list If the buyer finds any items to be missing or damaged do not hesitate to contact the distributor After the acceptance check is passed the buyer should fill out the check form and send its...

Page 7: ...tions 6 Chapter Three Preparations 8 1 Structure Description 8 2 Keyboard Diagram 9 3 Keyboard Instructions 9 4 Installation of Block Unit 10 4 1 Installation steps 10 4 2 Dismantlement Steps 11 5 Ins...

Page 8: ...6 1 Software installation 36 2 Online setup 36 3 Software installation 36 4 Configure your equipment 38 4 1 Network Connection 38 4 2 Network Enable Adminastrator 39 4 3 Configure 40 5 Main interface...

Page 9: ...oncology genetics forensics and immunology PCR technology is a milestone in the history of modern molecular biology 2 PCR Applications Research Areas Gene cloning DNA sequencing mutation analysis gen...

Page 10: ...ng components manufactured under Japanese quality controlling mode BOECO TC PRO Thermal Cycler possess the stable and reliable performance With speedy temperature ramping rate at the maximum of 4 S BO...

Page 11: ...CR experiments requirements It holds a leading position in the field of similar products BOECO TC PRO Thermal Cycler Adaptation Carrier BLOCK 96G 96 0 2ml Gradient block BLOCK 3048 30 0 5ml 48 0 2ml B...

Page 12: ...BOECO TC PRO Thermal Cycler Chapter One Introduction 4 BLOCK 4I 4pcs In Situ plate In Situ plate Standard Centrifuge Tube 0 2ml 2...

Page 13: ...orking conditions are satisfied Pay special attention to the power line s reliable grounding 2 Conditions for Transportation and Storage Ambient Temp 20 55 Relative Humidity 80 3 Basic Parameters Mode...

Page 14: ...ure of hot lid showed in the display would be higher than the set value 4 Performances Maximum number of cycles 99 Maximum number of sections 5 Maximum procedures within a cycle 16 Number of programs...

Page 15: ...via RJ45 interface Sound alarm Estimating total program execution time and remaining program running time Date and time year month day hour minute second display and calibration Failure protection and...

Page 16: ...the keyboard and each button s functions and some preparations before power on Before any operation please read this chapter carefully and make sure you are familiar with the steps introduced 1 Struct...

Page 17: ...tions are as follows 250V 8A 5 20mm The fuse should be replaced by one that meets those specifications If you need certificated fuses please contact the distributor or manufacturer 2 Keyboard Diagram...

Page 18: ...screen when setting documents Menu press any button to accept the current items displayed above the button 4 Installation of Block Unit The main unit must link with the block unit so as to work norma...

Page 19: ...damaged 3 When uninstall block please draw the block from the slot gently It s forbidden to lean the block for fear of snap of connection wire 4 After installation the block unit will be recognized by...

Page 20: ...le 6 Power on Turn on the power i e turn the power switch to position the BOECO TC PRO Thermal Cycler will beep twice and the fan start to running which means the instrument is power on At this time h...

Page 21: ...eated lid will stably press on the top of the sample tubes so that both the block s load and the temperature distribution in each tube are even Note Before closing the cover please revolve the knob co...

Page 22: ...te of Hot lid On the central area it displays Boeckel Co GmbH Co KG information software version number block model default file default user control mode and sample volume Press File to enter into fi...

Page 23: ...ontrol Mode is Block mode and press Accept running into the file operation interface If the current control mode is Tube mode press Accept key then pop up tube vol Settings dialog box In this interfac...

Page 24: ...nterface to enter file list interface There are 3 files listed in the form as showed in the picture When the flashing bar is flashing in the User Column select users through pop up key at this time th...

Page 25: ...round Press Digit Letter to modify parameter settings Press Function to change Press Seg to enter into segment edit interface In every segment the user can set setting temperature Temp duration Time R...

Page 26: ...arning 1 For the new edited PCR program please save first and then run it otherwise the file file can t be run 2 When setting Time as the hot lid will be closed automatically in the case that the bloc...

Page 27: ...emind File have been saved Please continue another operation Press Enter key to cancel the reminding bar If the file was used the system will remind you to rename Rename or continue to save Save Passw...

Page 28: ...he reminding information the user can select Start to continue If the user doesn t make a decision within 100 seconds the system will continue running automatically Press View File to view edited PCR...

Page 29: ...e relation between block temperature and reagent temperature Tube mode has considered the phenomenon that the cooling heating rate is a little slower than that of block There is an overshoot after rea...

Page 30: ...f the self testing is successful the screen will display the main interface Dual block means two blocks Block A and B respectively represent actual block on the left and right Press Shift to operate A...

Page 31: ...files listed in the form as showed in the picture When the flashing bar is flashing in the User Column select users through pop up key at this time the File Name Column will display the relative files...

Page 32: ...rameter setting interface In this interface move the cursor by pressing Up Down and Right Left and the position that the cursor reaches will be displayed in white on a black background Press Digit Let...

Page 33: ...the file When the file is normally saved the system will remind File have been saved Please continue another operation Press enter key to cancel the reminding bar If the file was used the system will...

Page 34: ...ng information to select STOP or START Press Pause and it will remind Now pause running According to the reminding information you can select START to continue If you don t make a decision within 100...

Page 35: ...ut 1 2 minutes and please wait patiently If the self testing is successful the screen will display the main interface Main Interface On the left top of the main interface the state of Hot lid is displ...

Page 36: ...en select relative user s file through pop up key Press Edit to edit the file indicated by the cursor Press New File to edit a new file Press Delete and the system will alarm Confirm delete file then...

Page 37: ...set cycle numbers and initial cycle segment The figure shows a cycle from the first segment to the third one and there are totally 10 times cycles Press Cycle repeatedly to add sections max 5 sections...

Page 38: ...to save the file When the file is normally saved the system will remind File have been saved Please continue another operation Press Enter to cancel the reminding bar If the file was used the system w...

Page 39: ...inal one NOTE After set the parameter please press Save to confirm setting otherwise the parameter remains the original one Before the instrument leaves the factory its inside clock has been adjusted...

Page 40: ...Input key to choice input file from usb flash memory the equipment display usb user list Press output key to choice export file to usb flash memory the equipment display the local file user list Gradi...

Page 41: ...ist Press Back key can return back to usb flash memory user list interface Press cursor up down moving key to choice the copay file If the file number bigger than 30 press cursor left right key to pre...

Page 42: ...return back to equipment user list interface Press cursor up down moving key to choice the copay file If the file number bigger than 30 press cursor left right key to previous next page If copy corre...

Page 43: ...folder Every user is one son folder of BOECO folder Forbid to insert and pull out usb flash memory very fast To protect apparatus and usb flash memory if insert and pull out usb flash memory the appa...

Page 44: ...omputer 1 Software installation Click Setup exe to lnstallation TC PRO Program 2 Online setup TC PRO has configurable embedded internal networking modules provide the corresponding tools set 3 Softwar...

Page 45: ...BOECO TC PRO Thermal Cycler Chapter Five Connection Computer 37 The third step choice installtion list click next button The fourth step click next button...

Page 46: ...nnection Computer 38 The fifths click install button The sixth step installation finished clich finish button 4 Configure your equipment 4 1 Network Connection Link the equipment and computer lines th...

Page 47: ...BOECO TC PRO Thermal Cycler Chapter Five Connection Computer 39 Connection 4 2 Network Enable Administrator Configure Mapping Search button Function list RJ45 connection Computer Instrument...

Page 48: ...tion Computer 40 4 3 Configure Click the Search button the device will search tool automatically Double click the searched IP on the column open the attribute page and choice the network option Search...

Page 49: ...pter Five Connection Computer 41 Open the attribute page and choice operrating mode option then click setting button The operating mode choice TCP server mode Single click ok button finish IP address...

Page 50: ...BOECO TC PRO Thermal Cycler Chapter Five Connection Computer 42 Single click function list COM MAPPING Click add object equipment button OK Add object equipment Delete object equipment...

Page 51: ...Cycler Chapter Five Connection Computer 43 Double click on the column searched IP setting the mapping attributes page select Advanced Settings setup as the following fig Choice Serial Parameters setup...

Page 52: ...s Note 1 The IP address is not unanimous when multiple devices interconnection 2 In the first use re equipped the equipment 3 Need to set up the same subnet when multiple equipment networking 4 Device...

Page 53: ...Checking the operating file 1 Document if the equipment running the device will be displayed from the bottom Run File A B Options 2 Double click Run File A B display the operation file content and equ...

Page 54: ...BOECO TC PRO Thermal Cycler Chapter Five Connection Computer 46 Check history information Double click the historical option in the System option to show the historical information...

Page 55: ...Five Connection Computer 47 Checking the equipment storage state Double click the System to view equipment storage condition Setup the system parameter Double click the setting option of the System o...

Page 56: ...How Create new users Change the user name Copy user Paste user Delete user Imput user Export user Create a new user 1 Choose the name of the equipment list or any user name 2 Choice the create user me...

Page 57: ...the ok button Clew User can t change the user name when the file of this user is opening Copy user 1 Choice the user name needed to copy 2 Choice the Copy User menu under User menu Paste user 1 Choose...

Page 58: ...computer 2 Choice the Import User menu under the User menu 3 Choice the imput list click the open button Clew Can t imput user if the existent user is opening Output user 1 Choice the user which want...

Page 59: ...e New File menu under File menu pop up the dialog box 3 Imput the file name and password click ok button Edit file 1 Double click the equipment list user option open the file list and double click edi...

Page 60: ...2 Choice the Copy File menu under File menu Paste File 1 Choose the user option under the equipment list 2 Choice the Paste File menu under File menu Clew Only can paste file between computer and equ...

Page 61: ...e the file which user want to change the name 2 Choice the Change File Name menu under File menu 3 Input file name click OK button Clew Can t change the opened file name Change the file password 1 Cho...

Page 62: ...ter modification Imput file 1 Choice the local computer user option 2 Choice Export File menu under the File menu 3 Choice the file click open button Clew Can t imput the opened file Export File 1 Cho...

Page 63: ...pment ip and name Connect equipment Choice the equipment click Connect menu Clew One equipment can only connect one computer at the same time Disconnection Choice the equipment click the Disconnect me...

Page 64: ...BOECO TC PRO Thermal Cycler Chapter Five Connection Computer 56...

Page 65: ...ng option under tool menu pop up the setting dialog box 2 Click the Add button to add a row 3 Click the Delete button to delete the current row 4 Put the ip address and alias click Save button to save...

Page 66: ...he Software 1 Open apparatus power to start instrument hold down the F1 key at the same time then equipment enter the software upgrade readiness estate the apparatus display Prepare to upgrade 2 Opera...

Page 67: ...er Chapter Six Firmware Upgrade 59 3 Configuration Click the Search button the device will search tool automatically Double click the searched IP address open the property page to choice the network o...

Page 68: ...mal Cycler Chapter Six Firmware Upgrade 60 Open Operrating Modemenu click settings open the property page to choice operrating mode and click setting button Choice Real Com Mode in operation Mode Choi...

Page 69: ...BOECO TC PRO Thermal Cycler Chapter Six Firmware Upgrade 61 Click the function list COM MAPPING option Click to add target equipment button OK Input target equipment Output target equipment...

Page 70: ...BOECO TC PRO Thermal Cycler Chapter Six Firmware Upgrade 62 Double click the searched IP address to set the mapping property page choice Advanced Setting as below Choice Serial Parameters as below...

Page 71: ...key the computer start to upgrade the software of instrument 7 Software under upgrade the apparatus will clue on Configuring Mode 8 After the software upgrade finish the apparatus will clue on Upgrad...

Page 72: ...apter Six Firmware Upgrade 64 Double click the searched IP address open the property page to choice the network option Operating mode choice TCP Server Mode Click Ok button close Network Enable Admina...

Page 73: ...Restart the apparatus after closing enter the system parameter configuration interface to reinstall system parameter Note Upgrade procedures will not be noticed in additional users can land website w...

Page 74: ...n 3 Error Message when running Please check error with any information below Temperature sensor disconnect Temperature sensor1 disconnect Temperature sensor2 disconnect Temperature sensor3 disconnect...

Page 75: ...t lid as OFF in the system parameter setting interface Set the hotlid s state as a certain temperature value The control temperature time is set as in the file edit interface which lead to the hotlid...

Page 76: ...ent with the same or different brand has their own control temperature features including cooling heating rate stability and fluctuation meanwhile the biological experiment has its own features such a...

Page 77: ...J3 3_ON OFF J6 ZJB 34P J5 CPU 34P CPU Board PCB2 BYQ505801400000 J1 POW 34P J7 CPU 34P J9 1_LAN J9 2_LAN J9 3_LAN J9 4_LAN J9 5_LAN J9 6_LAN J8 1_MOT1 J8 2_MOT2 J8 3_MOT3 J8 4_MOT4 J2 1_SW J2 2_SW J2...

Page 78: ...ference 0 30 8 Date Date YY MM DD 00 99 01 12 01 31 year month date 9 Time Time HH MM SS 00 00 00 23 59 59 hour minute second 10 Default File The default file name Max length is 11 bytes 11 Default Us...

Page 79: ...BOECO TC PRO Thermal Cycler Appendix 2 71 Fig 2 Fig 1 Block Reagent Block Reagent...

Page 80: ...NOTE...

Page 81: ...Boeckel Co GmbH Co KG P O Box 11 22 26 Roedingsmarkt 33 20459 Hamburg 20422 Hamburg Germany Tel 49 40 32 56 27 27 Fax 49 40 32 56 27 41 Website www boeco com...

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