Biotek Synergy HT, Operator'S Manual

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Error Codes
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179
Synergy HT Operator's Manual
Code Description and Probable Causes:
0600
Filter gain out of range
Fail if PMT saturation is not met within 3 ms.
This error indicates that during fluorescence autocalibration, the gain was increased to an unsafe
level, and the PMT analog PCB did not reach saturation.
Probable Causes:
•
The autocalibration jig is incorrectly placed in the carrier. The mirrors are not facing in the
correct direction.
•
The EM autocalibration jig is not connected to the PMT analog PCB.
0601 - 0606
Time-Resolved function reference channel gain out of range for the selected
wavelength
Fail if reference signal > 40000 and gain = 1.
This error indicates that the absorbance reference channel gain for a specific wavelength during
Time-resolved readings is out of the range necessary to ensure the lambda performance to
specifications. The second to last number is the lambda table position number.
Probable Causes:
•
The absorbance reference channel PCB is defective.
•
Order-sorting filter wheel is jammed not aligning the correct bandpass filter with the light
path, or the through hole is lined up, allowing white light to pass.
•
Monochromator is defective.
•
Flash lamp, alignment, or Flash lamp power supply is defective.
•
Lamp is too bright.
0610 - 0660 Time-Resolved function reference channel gain out of range for the selected
wavelength
Fail if Reference signal > 40000 and Gain = 1.
Note:
The order of the last two digits is <Filter> <Channel>. This is not consistent with other
error codes.
This error indicates that the absorbance reference channel gain for a specific wavelength during
Time-Resolved readings is out of the range necessary to ensure the lambda performance to
specifications. The second to last number is the lambda table position number.
Probable Causes:
•
The absorbance reference channel PCB is defective.
•
Order-sorting filter wheel is jammed, not aligning the correct bandpass filter with the light
path, or the through hole is allowing white light to pass.
•
Monochromator is defective.
•
Flash lamp, alignment, or Flash lamp power supply is defective.
•
Lamp is too bright.
0611 - 0616 Measurement channel gain out of range for the selected wavelength
Fail if Air reading > 60000 with a Gain = 1.
This error indicates that during a filter calibration or testing the reader prior to a read, one of the
filters saturated the measurement channel. The last number is the lambda table position
number.
Probable Causes:
•
The monochromator mirror/grating is damaged.
•
Order-sorting filter wheel is jammed, not aligning the correct bandpass filter with the light
path; the filter is degraded and not passing enough light energy, or filter is blocking the
light.
•
Absorbance analog PCB has intermittently failed.
•
Missed flashes or an erratic flash lamp.
•
Voltage to the lamp has decreased due to failure of the lamp power supply, or the motor
power supply PCB sent the wrong voltage request to the lamp power supply.