6 |
50™ TS Washer
Materials
•
One
new
microplate per test to be performed:
Microplate Type
Liquid Tests
8, 8s, 12, 2x8 Well Manifold Tests
Flat-bottom 96-well plates,
Corning® Costar #3590 (or
equivalent)
Dispense Precision Test
Evacuation Efficiency Test
Annual Buffer Switching Test (if applicable),
one plate per valve tested
4 Well Manifold Tests
24-well Corning 3524 plate (or
equivalent)
Dispense Precision Test
Evacuation Efficiency Test
8,16 Well Manifold Tests
Flat-bottom 384-well plates,
Corning Costar
(or equivalent)
Dispense Precision Test
Evacuation Efficiency Test
Annual Buffer Switching Test (if applicable),
one plate per valve tested
50 TS/8F and 8MF Models Test
96-well filter plate, Millipore®
MSHVN4550 96 (or equivalent 0.45
micron filter plate)
Vacuum Filtration Evacuation Efficiency
Test
•
Precision balance with capacity of 100 g minimum and readability of 0.001
g resolution
•
Pipettes and graduated beakers
•
Microplate absorbance reader capable of dual wavelength reading at 630
and 450 nm
•
Liquid Test Worksheets at the end of this chapter for recording data and
results
•
Deionized water
Test solutions
Solution #1: 0.1% Tween 20 in deionized water
•
Pipette 1 mL Tween 20 into 1 liter (1000 mL) of deionized water and mix
well, or
•
Pipette 10 mL of BioTek Solution #1 100X Concentrate Wetting Agent (PN
7773002) into 1 liter of deionized water and mix well.