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BioTek Instruments, Inc.
Residual Volume (Evacuation Efficiency)
Manifold
Type
Plate
Type
Performance
8 and 8s
Well
96-well Average residual of ≤2.0 µL per well after a 3-cycle wash when
dispensing 300 µL-per-well using a bottom touching well
aspiration and a solution of deionized water with 0.1%
Tween® 20 or equivalent buffer solution. (The aspirate height
should be optimized for the plate prior to testing.)
12 Well
96-well
Average residual of ≤2.0 µL per well after a 3-cycle wash when
dispensing 300 µL-per-well using a bottom touching well
aspiration and a solution of deionized water with 0.1%
Tween® 20 or equivalent buffer solution. (The aspirate height
should be optimized for the plate prior to testing.)
8,16 Well 384-well
Average residual of ≤4.0 µL per well after a 1-cycle wash when
dispensing 100 µL per well using a bottom touching well
aspiration and a solution of deionized water with 0.1% Tween
20 or equivalent buffer solution. (The aspirate height should be
optimized for the plate prior to testing.)
2x8 Well 96-well Average residual of ≤4.0 µL per well after a 3-cycle wash when
dispensing 300 µL per well using a bottom touching well
aspiration and a solution of deionized water with 0.1% Tween
20 or equivalent buffer solution. (The aspirate height should be
optimized for the plate prior to testing.)
4 Well
24-well
Average residual volume in the microwells shall be <50 µL per
well after 1120 µL is dispensed per well , using a near bottom
aspiration with a 2000 msec delay, using flat bottomed wells,
and deionized water with 0.1% Tween 20 wash solution.
Vacuum
Filtration
(for "F"
models)
96-well
Average increased weight of the plate is less than 1.2 grams
after dispensing 300 µL of deionized water per well into a
Millipore® MSHVN4450 96-well 0.45µm plates (PN 98258) and
vacuum aspirated for 30 seconds at vent diameter 0.047” (no
plug) and blotted on a paper towel.
