Biotage ELSD-1080, Operation Manual

Page: 36 / 59

Biotage ELSD-1080
temperature is reduced to ambient and sub-ambient
temperatures, the gas flow needs to be increased to
compensate (e.g. 1.6-1.8 SLM).
The evaporation gas range for both models is: 0.9-3.25 SLM
(0.05 increments). The default value is 1.6 SLM.
3.4.5 Detector Gain (PMT)
This parameter sets the factor by which the detector output
signal is amplified. The gain setting does not change the
sensitivity of the detector, but merely amplifies or divides the
captured signal by the inputted factor. The gain can be
adjusted from 1 to 10 in increments of 0.1.
When setting the PMT (or Gain), both the signal and noise are
simply amplified by the value set, so S/N values are unaffected.
The raw signal output displayed on the parameter screen will
reflect this increase or decrease in signal amplification.
Please note that the detector output displayed on the main
operating screen does not alter following a PMT change, thus
the recorded baseline position will remain unchanged.
Confirmation of a PMT change will be obvious by the change in
baseline noise.
3.4.6 Response Time (Smoothing)
The data outputted from the detector can be averaged to
produce a smoother response. The smoothing width is set to
the number of data points over which the data is averaged and
can be regarded as a digital time constant. The smoothing
range is settable from 1-50, (in increments of 1) which
translates to 0.1-5.0 seconds.
For flash applications a value of 50 (5 seconds) is satisfactory.
3.4.7 Light Source Intensity (LED)
The detector’s LED intensity can be adjusted in order to bring
the peak response back on-scale. The intensity range can be
set between 0-100%, with the default factory setting being
100%, for maximum sensitivity. The LED setting is stored in
memory and is retained even after a power on/off cycle.
This feature is extremely useful for preparative chromatography
where samples of high concentration can be analyzed which
would otherwise exceed the dynamic range of the detector.
Please note that the detector performs an automatic auto-zero
(i.e. 10mV) following an LED change in order to keep the signal
on-scale.
3.4.8 Power Mode
The detector can be configured from the front panel sub-menu
(see Section 3.3.4, page 33), to start in either RUN or
STANDBY mode when the unit is switched on via the rear
power button.
To configure the
Power Mode , select the required Status Mode
36 Publication date: July 2010