Biotage ELSD-1080, Operation Manual

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Biotage ELSD-1080
The liquid inlet port is connected directly to the nebulizer by a
short length (130 mm) of capillary tube giving a delay volume
from port to nebulizer tip of ~4 µL.
2.6.2 Outlet
The waste eluent is expelled from the detector via the front
outlet tube. The outlet tube should be connected to the 500-mL
waste bottle using the supplied Tygon
®
tubing of 40 mm ID.
For long periods of use, it is recommended that a larger solvent
container be used to prevent the solvent overflowing.
Warning
Do not allow the solvent waste tube to be submersed
below the solvent level inside the container. This will
cause positive pressure to be exerted on the nebulizer
chamber causing excessive baseline noise.
2.7 Connecting the Detector to Your LC System
To connect the detector:
1. Connect the power cord to the IEC inlet at the rear of
the unit.
2. Check the operating voltage of your detector, 110V 2A
or 230V 2A, on the IEC inlet fuse holder on rear of unit.
3. Attach the 4 mm OD gas tube into the gas inlet port at
the rear of the detector (see Figure 2). The gas
connection is a push-in fitting.
4. The gas should be dry, filtered and have a minimum
inlet pressure of 60 psi, 60-100 psi is required to
achieve the maximum operating detector gas flow rate
of 3.25 SLM.
5. Connect the solvent waste tube to the waste outlet at
the front of the detector and position the other end into
a waste collection bottle.
6. The solvent waste container supplied with the detector
can be sited on the bench in front of the detector.
Alternative waste collection vessels can be used if
required but the bottom of the waste tube must be
below the height of the waste outlet from the detector.
Waste tube must NOT be submerged in the liquid.
7. Connect the exhaust hose between the exhaust outlet
and a fume hood.
8. For analog data collection, connect the ELSD-1080
detector to the Isolera system using the analog cable
provided.
9. Connect the flash cartridge outlet to the flow splitter and
the splitter outlet capillary tube to the front of the unit
using the tubing provided in the flow splitter kit.
26 Publication date: July 2010