60
7.2. Dispensing without Blow-out
The pipette will not perform the blow-out function when using the reverse
pipetting and dispensing modes. Therefore, it is recommended that
dispensing is always performed with the tip set against the wall or the
bottom of the container. The use of the reverse pipetting mode is especially
useful when pipetting small volumes or solutions that have a tendency to
foam or have a high viscosity.
7.3. Other Recommendations
• Hold the pipette vertically when aspirating the liquid and place the tip
only a few millimetres into the liquid.
• Pre-rinse the tip before aspirating the liquid by
fi
lling and emptying the
tip for three to
fi
ve times. This is important especially when dispensing
liquids with a viscosity and density greater than water or liquids with
high vapor pressure (e.g. ethanol).
• When pipetting liquids with temperatures different to the ambient
temperature do not pre-rinse the tip and change the tip after each
pipetting.
• Check the pipette, tip and liquid are at room temperature.
• Avoid contaminating the tip cone, do not rest the pipette on its side.
• Always place the pipette in the charging stand or carousel when it is
not in use.
• Never strike the tip cone against the tip tray when mounting tips as this
can damage several internal components.
• Do not drop the pipette, the charging stand, carousel or AC-adaptor as
this may cause excessive shock.
• Avoid exposing the unit to extreme temperature changes, humidity and
dust (operating temperature from 15°C to 40°C).
• Avoid rough handling, moderate pressure is all that is required when
using the keyboard.
• Avoid leaving the pipette on its side with liquid in the tip which might
seep back into the mechanism.
• Always pipette against the inside wall of the receiving vessel. Remove
the tip by drawing it up against the inside wall.
• Change
the
fi
lter on the tip cone regularly (after 50-250 pipettings).
8. STORAGE
When not in use it is recommended that the pipettor is stored in the ON
position in the Biohit Proline charging stand or carousel.
