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LIGHTWAVE 3 & 3+ SPECTROPHOTOMETERS USER’S MANUAL

TABLE OF CONTENTS

5061-058-REV-11

WARRANTY AND REPAIR

Warranty Policy

SAFETY INFORMATION

Hazards and Warnings

INTRODUCTION

The Biochrom WPA Spectrophotometers

INSTALLATION

Unpacking 9

Positioning 9

Installing 9

INSTRUMENT OVERVIEW

Scope 10

Spectrophotometer Principle and Intended Use

Hardware 10

Technical Specifications

Display and Keypad

Instrument Connections

PVC PC Software

Biochrom Resolution PC Software

Instrument Data Output

Performing a Measurement

USER INTERFACE

Keypad 13

Instrument Firmware

Home Screen

Applications 14

Single Wavelength

Concentration

Wavescan

Summary of Contents for WPA Lightwave 3

Page 1: ...Lightwave 3 Lightwave 3 Spectrophotometers Users Manual 5061 084 REV 1...

Page 2: ...ing 9 Positioning 9 Installing 9 INSTRUMENT OVERVIEW 10 Scope 10 Spectrophotometer Principle and Intended Use 10 Hardware 10 Technical Specifications 11 Display and Keypad 11 Instrument Connections 11...

Page 3: ...d USB Memory Stick 28 Settings 30 Date and Time 30 Regional 31 Printer 31 Preferences 31 Contrast 32 About the Instrument 32 Additional Options 33 Status Bar Icons 35 USEFUL CALCULATIONS 36 Beer Lambe...

Page 4: ...pport Email support hbiosci com Telephone 44 0 1223 423723 Address 1020 Cambourne Business Park Cambourne Cambridge UK CB23 6DW Goods will not be accepted for return unless the RMA Return Materials Au...

Page 5: ...or the biohazard in question Rinse with distilled water Allow to dry thoroughly before reuse To further reduce the possibility of biohazards Include an appropriate decontamination certificate for equi...

Page 6: ...ical environments they are equally as relevant in agriculture animal husbandry and fishery geological exploration food safety environmental monitoring and many manufacturing industries to name a few T...

Page 7: ...ument for air to circulate freely The instrument should be positioned so that the power supply cable may be readily removed in the event of a hazard or malfunction Locate the instrument in an atmosphe...

Page 8: ...smission of light through a sample Samples absorb light based on their unique molecular composition The amount of absorbance is directly proportional to the sample concentration and the pathlength whi...

Page 9: ...340 nm Stability 0 01 A h at 340 nm Noise 0 005 peak to peak 0 002 RMS Digital Output USB Flash Drive PC via PVC software Optional Bluetooth Data Export USB Flash Drive tsv native PVC format PC via P...

Page 10: ...5 mm The light path is directed from RIGHT to LEFT through the cell chamber The cell holder supplied with the instrument accepts standard quartz glass or plastic cuvettes with pathlengths up to 40 mm...

Page 11: ...he snap dome switch keys of the membrane keypad offering tactile feedback during operation Instrument Firmware The instrument firmware uses an intuitive menu arrangement that is navigated using the me...

Page 12: ...ngs can be accessed from here Applications The Applications screen is accessed from the home screen using the 1 key It contains basic applications with definable parameters to meet the needs of typica...

Page 13: ...rrow select the mode Absorbance or Transmission using the left and right arrow keys Step 3 Press the down arrow set the sample prompt Yes or No to bring up the sample number option before each measure...

Page 14: ...e options key see the Additional Options section Concentration The Concentration application is accessed from the Applications screen using the 2 key It can be used to apply a known factor or one dete...

Page 15: ...ber of decimal places to display the concentration in Auto 0 1 or 2 using the left and right arrow keys Implement the changes and return to the parameters screen by selecting OK using the confirm key...

Page 16: ...mple then take a standard measurement using the confirm key Confirm the concentration of the standard sample by selecting OK using the confirm key OR Change the concentration of the standard sample by...

Page 17: ...ons section Wavescan The Wavescan application is accessed from the Applications screen using the 3 key It can be used to perform absorbance A or transmission T measurements across a range of wavelengt...

Page 18: ...equent sample measurements Step 7 Replace the previous sample with a test sample then take a sample measurement using the confirm key Repeat for all samples WARNING Previous measurements cannot be rec...

Page 19: ...keys Step 4 Press the down arrow set the interval between individual measurements 5 to 60 seconds using the left and right arrow or alphanumeric keys Step 5 Proceed to the second parameters screen by...

Page 20: ...t and right arrow keys Implement the changes and return to the parameters screen by selecting OK using the confirm key OR Reject the changes and return to the parameters screen by selecting Cancel usi...

Page 21: ...USB flash drive before any subsequent samples are measured Results The results are displayed on the screen Use the left and right arrow keys to move the cursor and display the absorbance at each inte...

Page 22: ...and right arrow keys Step 3 Press the down arrow enter up to 8 digits to define the units that the primary result will be reported in OR Open the units options using the options key Select one of the...

Page 23: ...standard samples are being used to create the standard curve only Zero Regression Regression and Interpolation fits can be selected Step 7 Press the down arrow select the source of the calibration St...

Page 24: ...le then take a reference measurement using the reference key The acquired reference sample baseline will be applied to all subsequent standard and test sample measurements Step 12 Replace the previous...

Page 25: ...he screen Return to the Applications screen using the escape key OR display the options menu using the options key see the Additional Options section Multi Wavelength The Multi Wavelength application...

Page 26: ...ompt Yes or No to bring up the sample number option before each measurement using the left and right arrow keys Repeat for all wavelengths Step 5 Proceed to the measurement screen by selecting OK usin...

Page 27: ...mpt Yes or No to bring up the sample number option before each measurement using the left and right arrow keys Step 3 Press the down arrow set the background On or Off using the left and right arrow k...

Page 28: ...ric keys Step 5 Enter any dilution factor to be applied to the absorbance measurement of a value of up to four significant figures using the alphanumeric keys OR Open the dilution factor options using...

Page 29: ...Repeat for all samples WARNING Previous measurements cannot be recalled so should be printed or output to a USB flash drive be fore any subsequent samples are measured Step 9 Insert the reference samp...

Page 30: ...the saved custom method application is accessed there are several options available OR return to the home screen using the escape key 1 To open a saved custom method application use the appropriate a...

Page 31: ...re All Folders using the left and right arrow keys For Backup Folder and Restore Folder operations press the down arrow and select the folder to perform the operation on Favourites Methods 1 Methods 2...

Page 32: ...s application Settings screen with the Games application hidden Step 1 Set the day using the left and right arrow or alphanumeric keys Step 2 Press the down arrow set the month using the left and righ...

Page 33: ...the Settings screen by selecting OK using the confirm key OR Reject the changes and return to the Settings screen by selecting Cancel using the escape key Step 1 Set auto print On of Off using the lef...

Page 34: ...arrow set the application menus layout theme using the left and right arrow keys Step 3 Press the down arrow set the parameter history to store application settings for future use or not using the lef...

Page 35: ...oggle between Absorbance and T mode Graph Displays the graph showing the measurements local spectrum Print Data Prints tabulated time point data About The About pop up window is opened from the Settin...

Page 36: ...ow and set the minimum peak height 0 00 to 9999 using the alphanumeric keys Then press the down arrow and set the minimum peak width 1 to 190 nm using the alphanumeric keys Then press the down arrow a...

Page 37: ...ght arrow keys Step 2 Press the down arrow apply x axis limits or not using the left and right arrow keys For On to x axis limits press the down key and set the x axis start value x1 using the alphanu...

Page 38: ...screen without saving the method by selecting Cancel using the escape key 8 Auto print to computer via Bluetooth is active Auto print to built in printer is active Auto print to computer via USB cable...

Page 39: ...n coefficients can be applied to calculate the concentration in alternative units c Molar extinction coefficient M 1cm 1 Molar or moles per litre concentration M mol L 1 Mass extinction coefficient g...

Page 40: ...erence sample Possible incorrect optical alignment Contact technical support Absorbance lower than expected Incorrect sample reference Check sample and reference for contamination Check sample and ref...

Page 41: ...eturn the instrument to its upright position and lift the accessory cover vertically upwards Step 3 Remove any tie wraps from the printer cable and insert its connector to the socket on the underside...

Page 42: ...ument and replace the accessory cover screws at A and B Step 1 Lift off the printer cover using the tabs and discard the spent roll Step 2 Open the platen lock feed in the paper so that the loose end...

Page 43: ...nformation Part Number Description 80 3007 42 WPA Lightwave 3 80 3007 43 WPA Lightwave 3 with Printer 80 3007 44 WPA Lightwave 3 with Bluetooth 80 3007 45 WPA Lightwave 3 with Printer and Bluetooth 80...

Page 44: ...spectros com Biochrom U S 84 October Hill Road Holliston Massachusetts 01746 USA phone 1 508 893 8999 toll free 1 877 246 2476 fax 1 508 429 5732 e mail support hbiosci com web www biochrom co uk www...

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