BIO RAD S3e, User Manual

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Contents
Brief Description ............................................................................................................................ 3
Reference ...................................................................................................................................... 3
Location ......................................................................................................................................... 3
Suggestion for “Materials and Methods” ..................................................................................... 4
Suggestion for “Acknowledgements” ........................................................................................... 4
People and Contacts...................................................................................................................... 4
Features and Specifications .......................................................................................................... 5
Lasers/Filter-Sets ........................................................................................................................... 6
Additional Equipment ................................................................................................................... 7
System Overview ........................................................................................................................... 7
WARNINGS and Advice ................................................................................................................. 8
Available Techniques ..................................................................................................................... 9
Set Up ............................................................................................................................................ 9
Sample Preparation ................................................................................................................... 9
Initial Set Up: Start- up and Fluidics Procedure (≈ 20 minutes) ............................................... 10
Initial Set Up: Quality Control (≈ 10 minute s) ......................................................................... 12
Final Set Up: Low-Pressure Cleaning ....................................................................................... 14
Final Set Up: Shut Down .......................................................................................................... 15
Acquisition ................................................................................................................................... 18
Sorting ......................................................................................................................................... 21
Other Guidelines ......................................................................................................................... 27
Swapping Fluidics During Sorting: “Hot Swap” ....................................................................... 27
Auto Compensation Wizard .................................................................................................... 29
Protocols and Workspace ....................................................................................................... 32
Further Protocols, Tutorials and Guidelines ............................................................................... 33