21
15.
Run a single stained compensation control for each parameter
16.
Set the event limit high enough to visualize a positive population, then collect
and save the file
17.
Use the
Auto Compensation
wizard to set compensation for multi-coloured
samples (see Auto Compensation Wizard)
Sorting
1.
Go to
File
->
New Protocol
or
Open Protocol
(see
2.
Create at least one histogram and dot plot by clicking in the
Histogram
and
Density
buttons of the
Home
tab
3.
If desired, adjust temperature control for sample input and collection area in the
Temperature Control
area of the
Instrument Control Panel
